Institution
Wageningen University and Research Centre
Education•Wageningen, Netherlands•
About: Wageningen University and Research Centre is a education organization based out in Wageningen, Netherlands. It is known for research contribution in the topics: Population & Sustainability. The organization has 23474 authors who have published 54833 publications receiving 2608897 citations.
Topics: Population, Sustainability, Agriculture, Climate change, Gene
Papers published on a yearly basis
Papers
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TL;DR: The sources of plastic particles in agroecosystems, the mechanisms, constraints and dynamic behaviour of plastic during aging on land, and the responses of soil organisms and plants at different levels of biological organisation to plastic particles of micro and nano-scale are discussed.
754 citations
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TL;DR: The human monoclonal CR6261 epitope could be developed for use as a broad spectrum agent for prophylaxis or treatment of human or avian influenza infections without prior strain characterization and could be applied in targeted vaccine strategies or in the design of novel antivirals.
Abstract: Background
The hemagglutinin (HA) glycoprotein is the principal target of protective humoral immune responses to influenza virus infections but such antibody responses only provide efficient protection against a narrow spectrum of HA antigenic variants within a given virus subtype. Avian influenza viruses such as H5N1 are currently panzootic and pose a pandemic threat. These viruses are antigenically diverse and protective strategies need to cross protect against diverse viral clades. Furthermore, there are 16 different HA subtypes and no certainty the next pandemic will be caused by an H5 subtype, thus it is important to develop prophylactic and therapeutic interventions that provide heterosubtypic protection.
Methods and Findings
Here we describe a panel of 13 monoclonal antibodies (mAbs) recovered from combinatorial display libraries that were constructed from human IgM+ memory B cells of recent (seasonal) influenza vaccinees. The mAbs have broad heterosubtypic neutralizing activity against antigenically diverse H1, H2, H5, H6, H8 and H9 influenza subtypes. Restriction to variable heavy chain gene IGHV1-69 in the high affinity mAb panel was associated with binding to a conserved hydrophobic pocket in the stem domain of HA. The most potent antibody (CR6261) was protective in mice when given before and after lethal H5N1 or H1N1 challenge.
Conclusions
The human monoclonal CR6261 described in this study could be developed for use as a broad spectrum agent for prophylaxis or treatment of human or avian influenza infections without prior strain characterization. Moreover, the CR6261 epitope could be applied in targeted vaccine strategies or in the design of novel antivirals. Finally our approach of screening the IgM+ memory repertoire could be applied to identify conserved and functionally relevant targets on other rapidly evolving pathogens.
753 citations
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Wageningen University and Research Centre1, University of Cambridge2, University of Edinburgh3, University of Illinois at Urbana–Champaign4, Aarhus University5, Wellcome Trust Sanger Institute6, Institut national de la recherche agronomique7, Illumina8, Iowa State University9, Agricultural Research Service10, University of Missouri11, United States Department of Agriculture12
TL;DR: The results of this study indicate the utility of using next generation sequencing technologies to identify large numbers of reliable SNPs and demonstrate that the PorcineSNP60 Beadchip is an excellent tool that will likely be used in a variety of future studies in pigs.
Abstract: Background: The dissection of complex traits of economic importance to the pig industry requires the availability of a significant number of genetic markers, such as single nucleotide polymorphisms (SNPs). This study was conducted to discover several hundreds of thousands of porcine SNPs using next generation sequencing technologies and use these SNPs, as well as others from different public sources, to design a high-density SNP genotyping assay. Methodology/Principal Findings: A total of 19 reduced representation libraries derived from four swine breeds (Duroc, Landrace, Large White, Pietrain) and a Wild Boar population and three restriction enzymes (AluI, HaeIII and MspI) were sequenced using Illumina’s Genome Analyzer (GA). The SNP discovery effort resulted in the de novo identification of over 372K SNPs. More than 549K SNPs were used to design the Illumina Porcine 60K+SNP iSelect Beadchip, now commercially available as the PorcineSNP60. A total of 64,232 SNPs were included on the Beadchip. Results from genotyping the 158 individuals used for sequencing showed a high overall SNP call rate (97.5%). Of the 62,621 loci that could be reliably scored, 58,994 were polymorphic yielding a SNP conversion success rate of 94%. The average minor allele frequency (MAF) for all scorable SNPs was 0.274. Conclusions/Significance: Overall, the results of this study indicate the utility of using next generation sequencing technologies to identify large numbers of reliable SNPs. In addition, the validation of the PorcineSNP60 Beadchip demonstrated that the assay is an excellent tool that will likely be used in a variety of future studies in pigs.
751 citations
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TL;DR: In this paper, it was shown that past behaviour, habit and hedonic appreciation are usually better predictors of actual food choice behavior than psychological constructs like attitudes and intentions, which should lead to a rethinking of the methods used in sensory and consumer research.
750 citations
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Harvard University1, University of British Columbia2, Centre national de la recherche scientifique3, Boston University4, United States Forest Service5, Institut national de la recherche agronomique6, University of Antwerp7, Max Planck Society8, Free University of Bozen-Bolzano9, Wageningen University and Research Centre10, Peking University11, Helmholtz Centre for Environmental Research - UFZ12, National Institute for Environmental Studies13, University of California, Berkeley14, Russian Academy of Sciences15
TL;DR: Investigation of relationships between phenology and productivity in temperate and boreal forests finds the productivity of evergreen needleleaf forests is less sensitive to phenology than is productivity of deciduous broadleaf forests, which has implications for how climate change may drive shifts in competition within mixed-species stands.
Abstract: We use eddy covariance measurements of net ecosystem productivity (NEP) from 21 FLUXNET sites (153 site-years of data) to investigate relationships between phenology and productivity (in terms of both NEP and gross ecosystem photosynthesis, GEP) in temperate and boreal forests. Results are used to evaluate the plausibility of four different conceptual models. Phenological indicators were derived from the eddy covariance time series, and from remote sensing and models. We examine spatial patterns (across sites) and temporal patterns (across years); an important conclusion is that it is likely that neither of these accurately represents how productivity will respond to future phenological shifts resulting from ongoing climate change. In spring and autumn, increased GEP resulting from an 'extra' day tends to be offset by concurrent, but smaller, increases in ecosystem respiration, and thus the effect on NEP is still positive. Spring productivity anomalies appear to have carry-over effects that translate to productivity anomalies in the following autumn, but it is not clear that these result directly from phenological anomalies. Finally, the productivity of evergreen needleleaf forests is less sensitive to phenology than is productivity of deciduous broadleaf forests. This has implications for how climate change may drive shifts in competition within mixed-species stands.
750 citations
Authors
Showing all 23851 results
Name | H-index | Papers | Citations |
---|---|---|---|
Walter C. Willett | 334 | 2399 | 413322 |
Albert Hofman | 267 | 2530 | 321405 |
Frank B. Hu | 250 | 1675 | 253464 |
Willem M. de Vos | 148 | 670 | 88146 |
Willy Verstraete | 139 | 920 | 76659 |
Jonathan D. G. Jones | 129 | 417 | 80908 |
Bert Brunekreef | 124 | 806 | 81938 |
Pedro W. Crous | 115 | 809 | 51925 |
Marten Scheffer | 111 | 350 | 73789 |
Wim E. Hennink | 110 | 600 | 49940 |
Daan Kromhout | 108 | 453 | 55551 |
Peter H. Verburg | 107 | 464 | 34254 |
Marcel Dicke | 107 | 613 | 42959 |
Vincent W. V. Jaddoe | 106 | 1008 | 44269 |
Hao Wu | 105 | 669 | 42607 |