Washington State University

About: Washington State University is a education organization based out in Pullman, Washington, United States. It is known for research contribution in the topics: Population & Gene. The organization has 26947 authors who have published 57736 publications receiving 2341509 citations. The organization is also known as: WSU & Wazzu.

Generalized Feedback Shift Register Pseudorandom Number Algorithm

Abstract: The generalized feedback shift register pseudorandom number algorithm has several advantages over all other pseudorandom number generators. These advantages are: (1) it produces multidimensional pseudorandom numbers; (2) it has an arbitrarily long period independent of the word size of the computer on which it is implemented; (3) it is faster than other pseudorandom number generators; (4) the “same” floating-point pseudorandom number sequence is obtained on any machine, that is, the high order mantissa bits of each pseudorandom number agree on all machines— examples are given for IBM 360, Sperry-Rand-Univac 1108, Control Data 6000, and Hewlett-Packard 2100 series computers; (5) it can be coded in compiler languages (it is portable); (6) the algorithm is easily implemented in microcode and has been programmed for an Interdata computer.

The effects of crossing trajectories on the dispersion of particles in a turbulent flow

Abstract: The effects of ‘crossing trajectories’ and inertia on the dispersion of particles suspended in a field of grid-generated turbulence were investigated experimentally. The effect of particle trajectories crossing the trajectories of fluid elements, under the influence of a potential field (usually gravity), is to force the particles from one region of highly correlated flow to another. In this manner, particles lose velocity correlation more rapidly than the corresponding fluid points and as a result disperse less.A homogeneous decaying turbulent field was created behind a square biplanar grid in a wind tunnel. Particles were charged by a corona discharge then passed into the test section through a small plastic tube. A uniform electric field within the test section was used to simulate the effect of gravity, forcing the charged particles out of regions of correlated fluid at a higher than normal rate, therefore inducing the effects of crossing trajectories. Two sizes of glass beads (5 μm and 57 μm diameter) were employed in order to observe inertial effects. Laser-Doppler anemometry was used to measure particle mean-square displacement, autocorrelation coefficient, and mean-square velocity, from which dispersion coefficients were calculated.For the two particle sizes used in the tests, it was found that the particle diffusion coefficient, after a suitably long time from their release, was influenced primarily by the effect of crossing trajectories. Only in the particle mean-square velocity was the particle inertia seen to have any effect. The ratio of the particle relaxation time to the Kolmogoroff timescale was found to be a good indicator for the effects of particle inertia.

Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions.

Abstract: Vibrio parahaemolyticus is an emerging bacterial pathogen capable of causing inflammatory gastroenteritis, wound infections and septicemia. As a food-borne illness, infection is most frequently associated with the consumption of raw or undercooked seafood, particularly shellfish. It is the primary cause of Vibrio-associated food-borne illness in the United States and the leading cause of food-borne illness in Japan. The larger of its two chromosomes harbors a set of genes encoding type III section system 1 (T3SS1), a virulence factor present in all V. parahaemolyticus strains that is similar to the Yersinia ysc T3SS. T3SS1 translocates effector proteins into eukaryotic cells where they induce changes to cellular physiology and modulate host-pathogen interactions. T3SS1 is also responsible for cytotoxicity towards several different cultured cell lines as well as mortality in a mouse model. Herein we used RNA-seq to obtain global transcriptome patterns of V. parahaemolyticus under conditions that either induce (growth in DMEM media, in trans expression of transcriptional regulator exsA) or repress T3SS1 expression (growth in LB-S media, in trans exsD expression) and during infection of HeLa cells over time. Comparative transcriptomic analysis demonstrated notable differences in the expression patterns under inducing conditions and was also used to generate an expression profile of V. parahaemolyticus during infection of HeLa cells. In addition, we identified several new genes that are associated with T3SS1 expression and may warrant further study.

Review: Ammonia emissions from dairy farms and beef feedlots1

Abstract: Hristov, A. N., Hanigan, M., Cole, A., Todd, R., McAllister T. A., Ndegwa, P. and Rotz, A. 2011. Review: Ammonia emissions from dairy farms and beef feedlots. Can. J. Anim. Sci. 91: 1–35. Ammonia emitted from animal feeding operations is an environmental and human health hazard, contributing to eutrophication of surface waters and nitrate contamination of ground waters, soil acidity, and fine particulate matter formation. It may also contribute to global warming through nitrous oxide formation. Along with these societal concerns, ammonia emission is a net loss of manure fertilizer value to the producer. A significant portion of cattle manure nitrogen, primarily from urinary urea, is converted to ammonium and eventually lost to the atmosphere as ammonia. Determining ammonia emissions from cattle operations is complicated by the multifaceted nature of the factors regulating ammonia volatilization, such as manure management, ambient temperature, wind speed, and manure composition and pH. Approaches to quanti...