Institution
Washington State University
Education•Pullman, Washington, United States•
About: Washington State University is a education organization based out in Pullman, Washington, United States. It is known for research contribution in the topics: Population & Gene. The organization has 26947 authors who have published 57736 publications receiving 2341509 citations. The organization is also known as: WSU & Wazzu.
Topics: Population, Gene, Catalysis, Context (language use), Poison control
Papers published on a yearly basis
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TL;DR: A better understanding of the function of these calmodulin target proteins should help in deciphering the Ca(2+)/calmodulin-mediated signal network and its role in plant growth, development and response to environmental stimuli.
531 citations
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TL;DR: Results indicate RNA silencing can be triggered by a variety of inducers and analyzed entirely using transient gene delivery systems, and indicate thatRNA silencing may be a significant limitation to expression of genes in the Agrobacterium-mediated transient assay but that this limitation can be overcome by using RNAsilencing suppressors.
Abstract: The Agrobacterium-mediated transient expression assay in intact tissues has emerged as a rapid and useful method to analyze genes and gene products in plants. In many cases, high levels of active protein can be produced without the need to produce transgenic plants. In this study, a series of tools were developed to enable strong or weak induction of RNA silencing and to suppress RNA silencing in the absence of stable transgenes. Transient delivery of a gene directing production of a double-stranded green fluorescent protein (GFP) transcript rapidly induced RNA silencing of a codelivered GFP reporter gene, effectively preventing accumulation of GFP protein and mRNA. RNA silencing triggered by the strong dsGFP inducer was partially inhibited by the tobacco etch virus silencing suppressor, P1/HC-Pro. In the absence of the strong double-stranded GFP inducer, the functional GFP gene served as a weak RNA silencing inducer in the transient assay, severely limiting accumulation of the GFP mRNA over time. The weak silencing induced by the GFP gene was suppressed by P1/HC-Pro. These results indicate RNA silencing can be triggered by a variety of inducers and analyzed entirely using transient gene delivery systems. They also indicate that RNA silencing may be a significant limitation to expression of genes in the Agrobacterium-mediated transient assay but that this limitation can be overcome by using RNA silencing suppressors.
531 citations
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TL;DR: The data suggest strongly that past glaciation profoundly influenced the genetic architecture of the flora and fauna of the Pacific Northwest, with populations surviving in several well-isolated northern and southern refugia and Pleistocence glaciation molded the intraspecific genetic Architecture of both plants and animals in a geographically similar manner.
Abstract: Molecular studies of plants from the Pacific Northwest of North America suggest a recurrent pattern of genetic differentiation and geographic structuring. In each of five angiosperms and one fern species representing diverse life histories, cpDNA data indicate two clades of populations that are geographically structured. A northern group comprises populations from Alaska to central or southern Oregon, whereas populations from central Oregon southward to northern California form a southern group. In several of these species, a few populations having southern genotypes may have survived in glacial refugia further north in the Olympic Peninsula, Queen Charlotte Islands, and Prince of Wales Island. Allozyme data reveal a similar pattern of differentiation in several other plants from the Pacific Northwest. North-south partitioning of genotypes has also been reported for several animal species from this region. On a broader geographic scale, northsouth partitioning of genotypes has also been observed in other plants from western North America having a variety of geographic distributions. Some species also display a reduction of genetic variability in the northern portion of their range compared to the south. The data suggest strongly that past glaciation profoundly influenced the genetic architecture of the flora and fauna of the Pacific Northwest. Two alternative hypotheses are advanced to explain the geographic structuring of genotypes. First, past glaciation may have created discontinuities in the geographic distributions of plant species, with populations surviving in several well-isolated northern and southern refugia. Following glaciation, migration of genetically differentiated, once-isolated populations resulted in the formation of a continuous geographic distribution with a major genetic discontinuity. Alternatively, plants survived and subsequently migrated northward from a southern refugium, and a genotype became fixed in one or a few populations at the leading edge of recolonization. Subsequent long-distance dispersal from this leading edge resulted in a relatively uniform northern genotype that differs from the southern genotype(s). Whatever the underlying mechanism, Pleistocence glaciation may have molded the intraspecific genetic architecture of both plants and animals from the Pacific Northwest in a geographically similar manner. Future studies should seek to obtain a comprehensive phylogeography for regions that includes a diversity of both plants and animals.
531 citations
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TL;DR: This article examined the construct validation results of 70 published data sets and found that traits account for less than 50% of the variance in construct measures, while on average, traits accounted for more than 80% of variance.
Abstract: The authors examine the construct validation results of 70 published data sets. The analysis shows that, on average, traits account for less than 50% of the variance in construct measures. These fi...
531 citations
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TL;DR: A bifacial model for the functional interaction of PsbS with photosystem II is proposed, based on the extent of qE inhibition in the mutants, photochemical and nonphotochemical quenching processes of Photosystem II were associated with distinct chlorophyll fluorescence life-time distribution components.
530 citations
Authors
Showing all 27183 results
Name | H-index | Papers | Citations |
---|---|---|---|
Anil K. Jain | 183 | 1016 | 192151 |
Martin Karplus | 163 | 831 | 138492 |
Herbert A. Simon | 157 | 745 | 194597 |
Suvadeep Bose | 154 | 960 | 129071 |
Rajesh Kumar | 149 | 4439 | 140830 |
Kevin Murphy | 146 | 728 | 120475 |
Jonathan D. G. Jones | 129 | 417 | 80908 |
Douglas E. Soltis | 127 | 612 | 67161 |
Peter W. Kalivas | 123 | 428 | 52445 |
Chris Somerville | 122 | 284 | 45742 |
Pamela S. Soltis | 120 | 543 | 61080 |
Yuehe Lin | 118 | 641 | 55399 |
Howard I. Maibach | 116 | 1821 | 60765 |
Jizhong Zhou | 115 | 766 | 48708 |
Farshid Guilak | 110 | 480 | 41327 |