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Wilmington University

EducationNew Castle, Delaware, United States
About: Wilmington University is a education organization based out in New Castle, Delaware, United States. It is known for research contribution in the topics: Electrical connector & Signal. The organization has 32921 authors who have published 31419 publications receiving 758664 citations. The organization is also known as: Wilmington College & WilmU.

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Journal ArticleDOI
01 Dec 2010
TL;DR: The Wide-field Infrared Survey Explorer (WISE) is mapping the whole sky following its launch on 14 December 2009 and completed its first full coverage of the sky on July 17 as discussed by the authors.
Abstract: The all sky surveys done by the Palomar Observatory Schmidt, the European Southern Observatory Schmidt, and the United Kingdom Schmidt, the InfraRed Astronomical Satellite and the 2 Micron All Sky Survey have proven to be extremely useful tools for astronomy with value that lasts for decades. The Wide-field Infrared Survey Explorer is mapping the whole sky following its launch on 14 December 2009. WISE began surveying the sky on 14 Jan 2010 and completed its first full coverage of the sky on July 17. The survey will continue to cover the sky a second time until the cryogen is exhausted (anticipated in November 2010). WISE is achieving 5 sigma point source sensitivities better than 0.08, 0.11, 1 and 6 mJy in unconfused regions on the ecliptic in bands centered at wavelengths of 3.4, 4.6, 12 and 22 micrometers. Sensitivity improves toward the ecliptic poles due to denser coverage and lower zodiacal background. The angular resolution is 6.1", 6.4", 6.5" and 12.0" at 3.4, 4.6, 12 and 22 micrometers, and the astrometric precision for high SNR sources is better than 0.15".

7,182 citations

Journal ArticleDOI
TL;DR: A screening window coefficient, called "Z- factor," is defined, which is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment.
Abstract: The ability to identify active compounds (³hits²) from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the ³suitability² of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the quality of assays directly. In this report, a screening window coefficient, called ³Z-factor,² is defined. This coefficient is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment. The Z-factor is a dimensionless, simple statistical characteristic for each HTS assay. The Z-factor provides a useful tool for comparison and evaluation of the quality of assays, and can be utilized in assay optimization and validation.

6,474 citations

Journal ArticleDOI
TL;DR: The MEK affinity of U0126, its selectivity for MEK over other kinases, and its cellular efficacy suggest that this compound will serve as a powerful tool for in vitro and cellular investigations of mitogen-activated protein kinase-mediated signal transduction.

3,064 citations

01 Jan 1979
TL;DR: A short history of HPLC can be found in this paper, where the authors present a detailed overview of the current state of the art in HPLC and its application in the literature.
Abstract: PREFACE. GLOSSARY OF SYMBOLS AND ABBREVIATIONS. 1 INTRODUCTION. 1.1 Background Information. 1.2 A Short History of HPLC. 1.3 Some Alternatives to HPLC. 1.4 Other Sources of HPLC Information. References. 2 BASIC CONCEPTS AND THE CONTROL OF SEPARATION. 2.1 Introduction. 2.2 The Chromatographic Process. 2.3 Retention. 2.4 Peak Width and the Column Plate Number N. 2.5 Resolution and Method Development. 2.6 Sample Size Effects. 2.7 RELATED TOPICS. References. 3 EQUIPMENT. 3.1 Introduction. 3.2 Reservoirs and Solvent Filtration. 3.3 Mobile-Phase Degassing. 3.4 Tubing and Fittings. 3.5 Pumping Systems. 3.6 Autosamplers. 3.7 Column Ovens. 3.8 Data Systems. 3.9 Extra-Column Effects. 3.10 Maintenance. References. 4 DETECTION. 4.1 Introduction. 4.2 Detector Characteristics. 4.3 Introduction to Individual Detectors. 4.4 UV-Visible Detectors. 4.5 Fluorescence Detectors. 4.6 Electrochemical (Amperometric) Detectors. 4.7 Radioactivity Detectors. 4.8 Conductivity Detectors. 4.9 Chemiluminescent Nitrogen Detector. 4.10 Chiral Detectors. 4.11 Refractive Index Detectors. 4.12 Light-Scattering Detectors. 4.13 Corona-Discharge Detector (CAD). 4.14 Mass Spectral Detectors (MS). 4.15 Other Hyphenated Detectors. 4.16 Sample Derivatization and Reaction Detectors. References. 5 THE COLUMN. 5.1 Introduction. 5.2 Column Supports. 5.3 Stationary Phases. 5.4 Column Selectivity. 5.5 Column Hardware. 5.6 Column-Packing Methods. 5.7 Column Specifications. 5.8 Column Handling. References. 6 REVERSED-PHASE CHROMATOGRAPHY FOR NEUTRAL SAMPLES. 6.1 Introduction. 6.2 Retention. 6.3 Selectivity. 6.4 Method Development and Strategies for Optimizing Selectivity. 6.5 Nonaqueous Reversed-Phase Chromatography (NARP). 6.6 Special Problems. References. 7 IONIC SAMPLES: REVERSED-PHASE, ION-PAIR, AND IONEXCHANGE CHROMATOGRAPHY. 7.1 Introduction. 7.2 Acid-Base Equilibria and Reversed-Phase Retention. 7.3 Separation of Ionic Samples by Reversed-Phase Chromatography (RPC). 7.4 Ion-Pair Chromatography (IPC). 7.5 Ion-Exchange Chromatography (IEC). References. 8 NORMAL-PHASE CHROMATOGRAPHY. 8.1 Introduction. 8.2 Retention. 8.3 Selectivity. 8.4 Method-Development Summary. 8.5 Problems in the Use of NPC. 8.6 Hydrophilic Interaction Chromatography (HILIC). References. 9 GRADIENT ELUTION. 9.1 Introduction. 9.2 Experimental Conditions and Their Effects on Separation. 9.3 Method Development. 9.4 Large-Molecule Separations. 9.5 Other Separation Modes. 9.6 Problems. References. 10 COMPUTER-ASSISTED METHOD DEVELOPMENT. 10.1 Introduction. 10.2 Computer-Simulation Software. 10.3 Other Method-Development Software. 10.4 Computer Simulation and Method Development. References. 11 QUALITATIVE AND QUANTITATIVE ANALYSIS. 11.1 Introduction. 11.2 Signal Measurement. 11.3 Qualitative Analysis. 11.4 Quantitative Analysis. 11.5 Summary. References. 12 METHOD VALIDATION. 12.1 Introduction. 12.2 Terms and Definitions. 12.3 System Suitability. 12.4 Documentation. 12.5 Validation for Different Pharmaceutical-Method Types. 12.6 Bioanalytical Methods. 12.7 Analytical Method Transfer (AMT). 12.8 Method Adjustment or Method Modification. 12.9 Quality Control and Quality Assurance. 12.10 Summary. References. 13 BIOCHEMICAL AND SYNTHETIC POLYMER SEPARATIONS. 13.1 Biomacromolecules. 13.2 Molecular Structure and Conformation. 13.3 Special Considerations for Biomolecule HPLC. 13.4 Separation of Peptides and Proteins. 13.5 Separation of Nucleic Acids. 13.6 Separation of Carbohydrates. 13.7 Separation of Viruses. 13.8 Size-Exclusion Chromatography (SEC). 13.9 Large-Scale Purification of Large Biomolecules. 13.10 Synthetic Polymers. References. 14 ENANTIOMER SEPARATIONS. 14.1 Introduction. 14.2 Background and Definitions. 14.3 Indirect Method. 14.4 Direct Method. 14.5 Peak Dispersion and Tailing. 14.6 Chiral Stationary Phases and Their Characteristics. 14.7 Thermodynamic Considerations. References. 15 PREPARATIVE SEPARATIONS. 15.1 Introduction. 15.2 Equipment for Prep-LC Separation. 15.3 Isocratic Elution. 15.4 Severely Overloaded Separation. 15.5 Gradient Elution. 15.6 Production-Scale Separation. References. 16 SAMPLE PREPARATION. 16.1 Introduction. 16.2 Types of Samples. 16.3 Preliminary Processing of Solid and Semi-Solid Samples. 16.4 Sample Preparation for Liquid Samples. 16.5 Liquid-Liquid Extraction. 16.6 Solid-Phase Extraction (SPE). 16.7 Membrane Techniques in Sample Preparation. 16.8 Sample Preparation Methods for Solid Samples. 16.9 Column-Switching. 16.10 Sample Preparation for Biochromatography. 16.11 Sample Preparation for LC-MS. 16.12 Derivatization in HPLC. References. 17 TROUBLESHOOTING. Quick Fix. 17.1 Introduction. 17.2 Prevention of Problems. 17.3 Problem-Isolation Strategies. 17.4 Common Symptoms of HPLC Problems. 17.5 Troubleshooting Tables. References. APPENDIX I. PROPERTIES OF HPLC SOLVENTS. I.1 Solvent-Detector Compatibility. I.1.1 UV Detection. I.1.2 RI Detection. I.1.3 MS Detection. I.2 Solvent Polarity and Selectivity. I.3 Solvent Safety. References. APPENDIX II. PREPARING BUFFERED MOBILE PHASES. II.1 Sequence of Operations. II.2 Recipes for Some Commonly Used Buffers. Reference. Index.

2,509 citations

Journal ArticleDOI
29 Nov 1996-Cell
TL;DR: Results provide direct genetic evidence that COX-2 plays a key role in tumorigenesis and indicate that COx-2-selective inhibitors can be a novel class of therapeutic agents for colorectal polyposis and cancer.

2,446 citations


Showing all 32930 results

David W. Johnson1602714140778
David Eisenberg156697112460
Joseph Schlessinger15049298862
Bruce R. Rosen14868497507
David A. Jackson136109568352
Donald B. Rubin132515262632
Michael A. Caligiuri12756173419
Alexander Rich11553950171
Rury R. Holman112502117528
Daniel C. Javitt11240639413
William F. DeGrado11059943508
Benjamin S. Hsiao10860241071
Xiao-Ming Chen10859642229
Onur Mutlu10354334806
Junwei Cao10347768110
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