Worcester Polytechnic Institute

About: Worcester Polytechnic Institute is a education organization based out in Worcester, Massachusetts, United States. It is known for research contribution in the topics: Population & Data envelopment analysis. The organization has 6270 authors who have published 12704 publications receiving 332081 citations. The organization is also known as: WPI.

Identification of a New Class of Antifungals Targeting the Synthesis of Fungal Sphingolipids

Abstract: Recent estimates suggest that >300 million people are afflicted by serious fungal infections worldwide. Current antifungal drugs are static and toxic and/or have a narrow spectrum of activity. Thus, there is an urgent need for the development of new antifungal drugs. The fungal sphingolipid glucosylceramide (GlcCer) is critical in promoting virulence of a variety of human-pathogenic fungi. In this study, we screened a synthetic drug library for compounds that target the synthesis of fungal, but not mammalian, GlcCer and found two compounds [ N ′-(3-bromo-4-hydroxybenzylidene)-2-methylbenzohydrazide (BHBM) and its derivative, 3-bromo- N ′-(3-bromo-4-hydroxybenzylidene) benzohydrazide (D0)] that were highly effective in vitro and in vivo against several pathogenic fungi. BHBM and D0 were well tolerated in animals and are highly synergistic or additive to current antifungals. BHBM and D0 significantly affected fungal cell morphology and resulted in the accumulation of intracellular vesicles. Deep-sequencing analysis of drug-resistant mutants revealed that four protein products, encoded by genes APL5 , COS111 , MKK1 , and STE2 , which are involved in vesicular transport and cell cycle progression, are targeted by BHBM. IMPORTANCE Fungal infections are a significant cause of morbidity and mortality worldwide. Current antifungal drugs suffer from various drawbacks, including toxicity, drug resistance, and narrow spectrum of activity. In this study, we have demonstrated that pharmaceutical inhibition of fungal glucosylceramide presents a new opportunity to treat cryptococcosis and various other fungal infections. In addition to being effective against pathogenic fungi, the compounds discovered in this study were well tolerated by animals and additive to current antifungals. These findings suggest that these drugs might pave the way for the development of a new class of antifungals.

Wearable vibrotactile systems for virtual contact and information display

Abstract: This paper presents a development history of a wearable, scalable vibrotactile stimulus delivery system. This history has followed a path from desktop-based, fully wired systems, through hybrid approaches consisting of a wireless connection from the host computer to a body-worn control box and wires to each tactor, to a completely wireless system employing Bluetooth technology to connect directly from the host to each individual tactor unit. Applications for such a system include delivering vibrotactile contact cues to users of virtual environments, providing directional cues in order to increase situational awareness in both real and virtual environments, and for general information display in wearable contexts. Through empirical study, we show that even a simple configuration, such as eight tactors arrayed around the torso, can be effective in increasing situational awareness in a building-clearing task, compared to users who perform the same task without the added cues.

Toward Accurate Human Tracking: Modeling Time-of-Arrival for Wireless Wearable Sensors in Multipath Environment

Abstract: For time-of-arrival-(TOA)-based indoor human tracking system, the wireless channel between human body surface and external reference node can be regarded as the source of inaccuracy. Since only the arrival time of direct path provides accurate range estimate, the nonline of sight caused by human body leads to undetectable direct path condition and thus results in a significant distance measurement error. In this paper, we measured TOA ranging error for indoor human tracking applications inside a typical office environment. A large number of TOA ranging samples was obtained and statistically analyzed. The TOA ranging error was modeled as a Gaussian random variable with the parameters, including position of target sensor, angle between human facing direction, and direction of transmitter–receiver, signal-to-noise ratio, and bandwidth of the system. As a validation of proposed model, excellent agreement has been found between empirical measurement and model-based software simulation.

Dynamics of homogeneous bubbly flows Part 2. Velocity fluctuations

Abstract: Direct numerical simulations of the motion of up to 216 three-dimensional buoyant bubbles in periodic domains are presented. The bubbles are nearly spherical and have a rise Reynolds number of about 20. The void fraction ranges from 2% to 24%. Part 1 analysed the rise velocity and the microstructure of the bubbles. This paper examines the fluctuation velocities and the dispersion of the bubbles and the ‘pseudo-turbulence’ of the liquid phase induced by the motion of the bubbles. It is found that the turbulent kinetic energy increases with void fraction and scales with the void fraction multiplied by the square of the average rise velocity of the bubbles. The vertical Reynolds stress is greater than the horizontal Reynolds stress, but the anisotropy decreases when the void fraction increases. The kinetic energy spectrum follows a power law with a slope of approximately 3:6 at high wavenumbers.