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Institution

Wrocław University of Technology

EducationWrocław, Poland
About: Wrocław University of Technology is a education organization based out in Wrocław, Poland. It is known for research contribution in the topics: Laser & Fuzzy logic. The organization has 13115 authors who have published 31279 publications receiving 338694 citations.


Papers
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Journal ArticleDOI
TL;DR: Quantitative EPR method was applied to characterise four types of humic acids derived from composts, soil, peat and soft brown coal, and it was recognised that the radical centres, which are enhanced by 'ammonia effect' are quenched in the formed HA-Cu(II) complexes.

89 citations

Journal ArticleDOI
TL;DR: In this article, the role of water in the mechanism of solute transport between aqueous phases, separated by a supported liquid membrane with ionic liquids, was evaluated using case-study solutes.

89 citations

Journal ArticleDOI
TL;DR: In this paper, the authors studied the behavior of the general nonparametric estimate for the unknown regression function g, where the weight function wni is of the form wni(x) = wni (x; xi(n), …, xn(n)).

89 citations

Posted ContentDOI
15 May 2020-bioRxiv
TL;DR: This work has revealed the molecular rules governing PLpro substrate specificity and provides a framework for development of inhibitors with potential therapeutic value or drug repositioning.
Abstract: In December 2019, the first cases of a novel coronavirus infection causing COVID-19 were diagnosed in Wuhan, China. Viral Papain-Like cysteine protease (PLpro, NSP3) is essential for SARS-CoV-2 replication and represents a promising target for the development of antiviral drugs. Here, we used a combinatorial substrate library containing natural and a wide variety of nonproteinogenic amino acids and performed comprehensive activity profiling of SARS-CoV-2-PLpro. On the scaffold of best hits from positional scanning we designed optimal fluorogenic substrates and irreversible inhibitors with a high degree of selectivity for SARS PLpro variants versus other proteases. We determined crystal structures of two of these inhibitors (VIR250 and VIR251) in complex with SARS-CoV-2-PLpro which reveals their inhibitory mechanisms and provides a structural basis for the observed substrate specificity profiles. Lastly, we demonstrate that SARS-CoV-2-PLpro harbors deISGylating activities similar to SARS-CoV-1-PLpro but its ability to hydrolyze K48-linked Ub chains is diminished, which our sequence and structure analysis provides a basis for. Altogether this work has revealed the molecular rules governing PLpro substrate specificity and provides a framework for development of inhibitors with potential therapeutic value or drug repositioning.

88 citations

Journal ArticleDOI
TL;DR: Laccase isolated from Trametes versicolor was immobilized in polyacrylamide gel and used for the conversion of 4-methyl-3-hydroxyanthranilic acid to 2-amino-4,6-dimethyl-3

88 citations


Authors

Showing all 13239 results

NameH-indexPapersCitations
Krzysztof Palczewski11463146909
Claude B. Sirlin9847533456
Marek Czosnyka8874729117
Alfred Forchel85135834771
Jerzy Leszczynski7899327231
Kim R. Dunbar7447020262
Massimo Olivucci6729214880
Nitesh V. Chawla6138841365
Edward R. T. Tiekink60196721052
Bobby G. Sumpter6061923583
Wieslaw Krolikowski5950412836
Pappannan Thiyagarajan5924510650
Marek Samoc5840111171
Lutz Mädler5823227800
Rafał Weron5828512058
Network Information
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202372
2022231
20211,579
20201,769
20191,753
20181,963