Institution
Wuhan University
Education•Wuhan, China•
About: Wuhan University is a education organization based out in Wuhan, China. It is known for research contribution in the topics: Computer science & Population. The organization has 92849 authors who have published 92882 publications receiving 1691049 citations. The organization is also known as: WHU & Wuhan College.
Topics: Computer science, Population, Catalysis, Feature extraction, Apoptosis
Papers published on a yearly basis
Papers
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TL;DR: In this paper, a new concept of energy-harvesting, the flutter-mill, is proposed in which these flutter motions are utilized to generate electrical power, based on the energy analysis of the fluid-structure interaction system.
256 citations
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TL;DR: Compared to the single photodynamic therapy (PDT) or photothermal therapy (PTT), the RB-GNRs with combined PDT-PTT capabilities provide better therapeutic effects against oral cancer and have large potential in cancer treatment.
256 citations
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TL;DR: It is indicated that miR-215, through the suppression of DTL expression, induces a decreased cell proliferation by causing G2-arrest, thereby leading to an increase in chemoresistance to MTX and TDX.
Abstract: Translational control mediated by non-coding microRNAs (miRNAs) plays a key role in the mechanism of cellular resistance to anti-cancer drug treatment. Dihydrofolate reductase (DHFR) and thymidylate synthase (TYMS, TS) are two of the most important targets for antifolate- and fluoropyrimidine-based chemotherapies in the past 50 years. In this study, we investigated the roles of miR-215 in the chemoresistance to DHFR inhibitor methotrexate (MTX) and TS inhibitor Tomudex (TDX). The protein levels of both DHFR and TS were suppressed by miR-215 without the alteration of the target mRNA transcript levels. Interestingly, despite the down-regulation of DHFR and TS proteins, ectopic expression of miR-215 resulted in a decreased sensitivity to MTX and TDX. Paradoxically, gene-specific small-interfering RNAs (siRNAs) against DHFR or TS had the opposite effect, increasing sensitivity to MTX and TDX. Further studies revealed that over-expression of miR-215 inhibited cell proliferation and triggered cell cycle arrest at G2 phase, and that this effect was accompanied by a p53-dependent up-regulation of p21. The inhibitory effect on cell proliferation was more pronounced in cell lines containing wild-type p53, but was not seen in cells transfected with siRNAs against DHFR or TS. Moreover, denticleless protein homolog (DTL), a cell cycle-regulated nuclear and centrosome protein, was confirmed to be one of the critical targets of miR-215, and knock-down of DTL by siRNA resulted in enhanced G2-arrest, p53 and p21 induction, and reduced cell proliferation. Additionally, cells subjected to siRNA against DTL exhibited increased chemoresistance to MTX and TDX. Endogenous miR-215 was elevated about 3-fold in CD133+HI/CD44+HI colon cancer stem cells that exhibit slow proliferating rate and chemoresistance compared to control bulk CD133+/CD44+ colon cancer cells. Taken together, our results indicate that miR-215, through the suppression of DTL expression, induces a decreased cell proliferation by causing G2-arrest, thereby leading to an increase in chemoresistance to MTX and TDX. The findings of this study suggest that miR-215 may play a significant role in the mechanism of tumor chemoresistance and it may have a unique potential as a novel biomarker candidate.
256 citations
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TL;DR: It is proposed that abnormal mitochondrial dynamics represents a key common pathway that mediates or amplifies mitochondrial dysfunction and neuronal dysfunction during the course of neurodegeneration.
256 citations
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TL;DR: In this paper, the amine functional groups in the COF-300-AR backbone facilitated the electrochemical reduction of CO2 on silver electrodes in a concerted manner and led to selective generation of CO.
256 citations
Authors
Showing all 93441 results
Name | H-index | Papers | Citations |
---|---|---|---|
Jing Wang | 184 | 4046 | 202769 |
Jiaguo Yu | 178 | 730 | 113300 |
Lei Jiang | 170 | 2244 | 135205 |
Gang Chen | 167 | 3372 | 149819 |
Omar M. Yaghi | 165 | 459 | 163918 |
Xiang Zhang | 154 | 1733 | 117576 |
Yi Yang | 143 | 2456 | 92268 |
Thomas P. Russell | 141 | 1012 | 80055 |
Jun Chen | 136 | 1856 | 77368 |
Lei Zhang | 135 | 2240 | 99365 |
Chuan He | 130 | 584 | 66438 |
Han Zhang | 130 | 970 | 58863 |
Lei Zhang | 130 | 2312 | 86950 |
Zhen Li | 127 | 1712 | 71351 |
Chao Zhang | 127 | 3119 | 84711 |