Xuzhou Institute of Technology

About: Xuzhou Institute of Technology is a education organization based out in Xuzhou, China. It is known for research contribution in the topics: Catalysis & Adsorption. The organization has 1696 authors who have published 1521 publications receiving 13541 citations.

milR4 and milR16 Mediated Fruiting Body Development in the Medicinal Fungus Cordyceps militaris.

Abstract: Cordyceps militaris readily performs sexual reproduction, thus providing a remarkably rich model for understanding the processes involved in sexual development. It could regulate expression of human genes by diet-derived miRNA-like RNAs (milRNAs). However, the study of miRNAs in C. militaris has been limited. In the present study, genes encoding Dicers, Argonautes, and RNA-dependent RNA polymerases were identified. Illumina deep sequencing was performed to characterize the milRNAs in C. militaris at asexual and sexual development stages. Total 38 milRNAs were identified and five milRNAs were validated by northern blot and qRT-PCR, out of which, 19 were specific for sexual development. Importantly, the fungi could not form fruiting bodies after disruption of milR4, while the perithecium was formed in advance after over-expression of milR4. Abnormal pale yellow fruiting body primordium, covered with abnormal primordium, was formed in the strain with miR16 disruption. Although no milR4 or milR16 target genes were identified, differential expression of many different genes involved in mycelium growth and sexual development (mating process, mating signaling, and fruiting body development) among these mutants were found. Overall, milRNAs play vital roles in sexual development in C. militaris.

A Simplified PWM Strategy for Three-Level Converters on Three-Phase Four-Wire Active Power Filter

Abstract: Presently, the neutral-point potential offset is an inherent problem for three-level converters on three-phase four-wire active power filters. The conventional three-dimensional space vector pulse width modulation (3-D-SVPWM) strategy is adopted as a normal solution to the problem, but the selection of space vector sectors for four-wire converter is both abstract and complicated in practice. This paper proposes a new PWM strategy accordingly to simplify the selection process and the calculation of duration. Compared to the conventional 3D-SVPWM, the proposed method locates the sector of optimal vector directly with the polarity of three-phase reference voltage. Then, by applying voltage–second balance rule, it can perform a fast calculation of the duration and generate PWM signals to control the switching devices’ on/off. As an approach to correct the imbalance dc-link capacitor voltage, this strategy reconstructs the switching sequence. A portion of state O duration is disassembled into states P and N to suppress the neutral-point potential offset. The proposed strategy is validated with a range of experiments. The results indicate that this simplified PWM strategy can effectively reduce the computational burden, and also the dc-link capacitor voltage is well controlled.