Yonsei University

About: Yonsei University is a education organization based out in Seoul, South Korea. It is known for research contribution in the topics: Population & Cancer. The organization has 50162 authors who have published 106172 publications receiving 2279044 citations. The organization is also known as: Yonsei.

Precise manipulation of chromosomes in vivo enables genome-wide codon replacement.

Abstract: We present genome engineering technologies that are capable of fundamentally reengineering genomes from the nucleotide to the megabase scale We used multiplex automated genome engineering (MAGE) to site-specifically replace all 314 TAG stop codons with synonymous TAA codons in parallel across 32 Escherichia coli strains This approach allowed us to measure individual recombination frequencies, confirm viability for each modification, and identify associated phenotypes We developed hierarchical conjugative assembly genome engineering (CAGE) to merge these sets of codon modifications into genomes with 80 precise changes, which demonstrate that these synonymous codon substitutions can be combined into higher-order strains without synthetic lethal effects Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes

Cyanine-based fluorescent probe for highly selective detection of glutathione in cell cultures and live mouse tissues.

Abstract: Glutathione (GSH) plays a crucial role in human pathologies. Near-infrared fluorescence-based sensors capable of detecting intracellular GSH in vivo would be useful tools to understand the mechanisms of diseases. In this work, two cyanine-based fluorescent probes, 1 and 2, containing sulfonamide groups were prepared. Evaluation of the fluorescence changes displayed by probe 1, which contains a 2,4-dinitrobenzenesulfonamide group, shows that it is cell-membrane-permeable and can selectively detect thiols such as GSH, cysteine (Cys), and homocysteine (Hcy) in living cells. The response of 1 to thiols can be reversed by treatment with N-methylmaleimide (NMM). Probe 2, which possesses a 5-(dimethylamino)naphthalenesulfonamide group, displays high selectivity for GSH over Cys and Hcy, and its response can be reversed using NMM. The potential biological utility of 2 was shown by its use in fluorescence imaging of GSH in living cells. Furthermore, probe 2 can determine changes in the intracellular levels of GSH ...

A New Approach for Health Monitoring of Structures: Terrestrial Laser Scanning

Abstract: This paper presents a new approach for the health monitoring of structures using terrestrial laser scanning (TLS). 3-D coordinates of a target structure acquired using TLS can have maximum errors of about 10 mm, insufficient for health monitoring of structures. A displacement measurement model to improve the accuracy of the measurement is offered. The model is tested experimentally on a simply supported steel beam. Measurements were made using 3 different techniques: 1) linear variable displacement transducers (LVDTs), 2) electric strain gages, and 3) a long gage fiber optic sensor. The maximum deflections estimated by the TLS model are less than 1 mm and within 1.6% of those measured directly by LVDT. Although GPS methods allow measurement of displacements only at the GPS receiver antenna location, the proposed TLS method allows measurement of the entire bridge's deformed shape, and thus a realistic solution for monitoring structures at both structure and member level. Furthermore, it can be used to create a 3-D finite element model of a structural member or the entire structure at any point in time automatically. Through periodic measurement of deformations of a structure/structural member and performing inverse structural analyses with measured 3-D displacements, a structure's health can be monitored continuously.