American Journal of Clinical Pathology
Oxford University Press
About: American Journal of Clinical Pathology is an academic journal published by Oxford University Press. The journal publishes majorly in the area(s): Medicine & Lymphoma. It has an ISSN identifier of 0002-9173. Over the lifetime, 19337 publications have been published receiving 551570 citations. The journal is also known as: AJCP.
Papers published on a yearly basis
TL;DR: Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the “Kirby-Bauer” method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously.
Abstract: In the words of the authors, the paper by A. W. Bauer et al., from the University of Washington in Seattle, on a standardized single-disk method for antibiotic susceptibility testing “. . . consolidate(s) and update(s) previous descriptions of the method and provide(s) a concise outline for its performance and interpretation.” Clinical microbiologists were relieved that finally a disk diffusion method had been standardized, could be used with ease, and provided reliable results as compared with minimum inhibitory concentration tests. The pivotal role of Hans Ericsson’s theoretical and practical studies (H. Ericsson and G. Svartz-Malmberg, Antibiot. Chemother. 6:41–74, 1959), as well as earlier reports by some of the authors of the publications cited, must be mentioned as a matter of fairness. Most of the recommendations given are still valid today even though some of the antimicrobial agents are obsolete, new ones have been added, some zone sizes had to be modified, and new media were designed for Haemophilus influenzae and Neisseria gonorrhoeae. Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the “Kirby-Bauer” method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously. ALEXANDER VON GRAEVENITZ
TL;DR: Cytochemical methods for chloroacetate esterase, nonspecific esterases, peroxidase, and metachromasia were used to identify monocytes and neutrophilic, basophilic, or eosinophilic granulocytes.
Abstract: Cytochemical methods for chloroacetate esterase, nonspecific esterase, peroxidase, and metachromasia were used to identify monocytes and neutrophilic, basophilic, or eosinophilic granulocytes. These methods are very simple, sensitive, and easily reproducible. Their reaction products are highly chromogenic and insoluble in most organic solvents. Any of these methods may be used independently or in combination with another cytochemical method to demonstrate a particular type of cell as desired. The specific aims and possible areas for clinical application of these cytochemical methods are discussed.
TL;DR: The pathologic basis of disease is determined by X-ray diffraction analysis of the granuloma with an Higgs–Cotran–Bouchut–Seiden ratio of 3:1.
Abstract: Robbins and Cotran pathologic basis of disease / , Robbins and Cotran pathologic basis of disease / , کتابخانه دیجیتال جندی شاپور اهواز
TL;DR: The method utilizing an avidin-biotin-peroxidase complex was found to be more sensitive than the unlabeled antibody (PAP) method and the applications of this technic are discussed.
Abstract: Avidin has an extraordinary affinity for the small-molecule vitamin biotin. Covalently coupling biotin or avidin to peroxidase molecules does not interfere with their normal biochemical functions. The avidin or biotin molecules, either peroxidase conjugated or unconjugated, can be brought to the antigen sites by means of an antiavidin antibody. Several immunohistochemical staining technics based on this principal have been described. The method utilizing an avidin-biotin-peroxidase complex was found to be more sensitive than the unlabeled antibody (PAP) method. This method involved four sequential staining procedures: (1) primary antibody (goat anti-human antigen); (2) secondary antibody (rabbit antigoat IgG) added in relative excess; (3) goat antiavidin antibody; (4) avidin-biotin-peroxidase complex. The applications of this technic are discussed.