Showing papers in "Analytical Biochemistry in 1972"

TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.

TL;DR: A simple colorimetric assay for catalase activity has been described using K2Cr2O7/acetic acid reagent to determine values of different enzyme sources and compared with the values obtained by titrimetric methods.

TL;DR: The reaction with p-hydroxybenzoic acid hydrazide (PAHBAH), can be used in a simple colorimetric method to detect less than 1 μg glucose or similar sugar.

TL;DR: The enthalpy of decomposition of hydrogen peroxide by catalase has been determined calorimetrically in isotonic saline solutions at 25°C Extinction coefficients are also reported for hydrogen perox solutions in the ultraviolet as discussed by the authors.

TL;DR: The equilibrium composition of a multicomponent crossreactive ligand-binding system is characterized by solving the mass action equations and several results in simulation and parameter fitting for radioimmunoassay, ion-protein reactions, etc., are derived from these equations.

TL;DR: A modification of the Lowry procedure for the rapid and reliable analysis of proteolipid protein involves the addition of sodium dodecyl sulfate to dissolve the lipid-containing protein samples or to maintain the water-soluble apoprotein in solution.

TL;DR: A methodology is described for the analysis of tissue SH components of a single tissue sample in terms of soluble and insoluble, reactive and unreactive, SH, using the SH reagent DTNB.

TL;DR: This assay employs a filter paper technique in which newly formed glycogen-14C is precipitated on paper squares using 66% ethanol while other radioactivity is removed by washing to find utility for assay of extremely small tissue samples.

TL;DR: A new assay procedure for cathepsin B1 is described, which is about 35 times more sensitive than that previously used, in which the substrate was the 4-nitroanilide.

TL;DR: The experimental conditions for a rapid and sensitive spectrophotometric assay for carnitine palMIToyltransferase (CPT) are described and the amount of reduced CoA liberated from palmitoyl CoA by CPT is quantitated using 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB).

TL;DR: The method has been applied to the complete separation of RNA fractions obtained after a preliminary gel electrophoresis of partial enzymic digests of 32P-labeled bacteriophage RNA.

TL;DR: Optimal conditions for nucleotide determination are described as well as the beneficial use of the DuPont luminescence biometer.

TL;DR: A filter paper assay was developed for measuring the activity of transamidating enzymes by the incorporation of radioactive amine substrates into proteins, analogous to those generally employed for monitoring the enzymic coupling of isotopic groups to proteins, such as in the ATP-dependent phosphorylation of casein or histone.

TL;DR: Electrophoretic separation of seven acidic glycosaminoglycans was accomplished by two-dimensional electrophoresis on a cellulose acetate strip and high resolving power and sensitivity make it possible to identify AGG in tissues in as small amounts as biopsy specimens.

TL;DR: A new blend of silicone stationary phases (CFC), which provides superior resolving power than hitherto obtained with single phases or earlier blends and use of a carrier gas helium, which is a superior carrier gas to the more commonly used nitrogen and argon.

TL;DR: Interference with the Lowry protein determination by sulfhydryl compounds cysteine, DTT, 2-mercaptoethanol, and reduced glutathione has been largely removed by adding H 2 O 2 to the alkaline copper solution containing the protein followed by heating for 10 min at 50° before adding phenol reagent.

TL;DR: A rapid quantitative method has been developed for determining specifically the amount of microtubule protein in cell extracts using a DEAE-cellulose filter paper assay and 3 H-colchicine and is linear up to 300 μg protein.

TL;DR: A modification of the ethidium bromide method of Le Pecq and Paoletti (1966) is described that allows single and concurrent quantitative determinations of DNA and RNA directly in homogenates of milligram and submilligram samples of cells and tissues.

TL;DR: This pattern suggests that progressive compaction of the protein bands at shorter migration distances is the responsible factor, preventing the stoichiometric uptake of dye molecules once a critical protein concentration has been exceeded.

TL;DR: The specificity of the test for phospholipase A can be further improved by incorporation of human erythrocytes into the egg yolk plates, and the diameter of this parameter can be used for the quantitation of the enzyme.

TL;DR: A new rapid stain fixative has been devised for gel electrofocusing that is based on acid treatment of Coomassie Brilliant Blue R250 and gives highly reproducible results comparable to presently accepted procedures.

TL;DR: A mathematical basis for radioimmunoassays and radio-ligand assays in multicomponent cross-reactive systems at equilibrium has been defined, formulated, and solved for the case of several antigens and several antibodies with different pairwise affinity constants.

TL;DR: Variations in response to different polyglutamates must be considered in evaluating the values of folate activity in natural products as assayed by L. casei.

TL;DR: The method is extremely sensitive: tritium-labeled digest derived from less than 1 μg of polynucleotide is required to evaluate the base composition, including most of the so-called minor or modified bases which are natural constituents of RNA.

TL;DR: It was found that equimolar quantities of the maltodextrins gave absorbance values with alkaline 3,5-dintrosalicylate that increased with the number of glucose units in the dextrin, and the effect of calcium on dinitrosalICylate reducing values has misled some workers into concluding that this ion affects α-amylase action patterns.

TL;DR: A standardized method has been developed for the rapid isolation of rat liver microsomes using Ca 2+ and its advantages over other available methods have been outlined and the important enzymes in the electron transport system were determined.

TL;DR: There is an excellent correlation between total nitrogen, determined by the micro-Kjeldahl method, and the optical density developed during the ninhydrin estimation, which shows that the weight of protein giving on hydrolysis an OD of 1.0 is constant for a number of proteins.