Showing papers in "Analytical Biochemistry in 1974"

TL;DR: The high sensitivity of this method permits detection of the small amounts of cyclic AMP formed at low enzyme concentrations or at early time points in kinetic studies.

TL;DR: Agarose can be activated by adding cyanogen bromide, dissolved in acetonitrile, to beads suspended in a solution of sodium carbonate, and the necessity for manual titration and the use of a pH meter are eliminated.

TL;DR: The two enzymic procedures yielded equivalent results with all tissues examined (brain, liver, muscle and polymorphonuclear leucocytes).

TL;DR: It is proposed that the shape of the autocorrelation function of the weighted residuals may serve as a convenient criterion for the quality of fit between the experimental data and the decay function obtained by analysis.

TL;DR: The automated continuous-flow system for the extraction and fluorometric analysis of histamine is accurate, specific, precise, rapid, and very sensitive.

TL;DR: The estimated ϵ205 is about three times better than using an average ϵ2051 mg/ml of 31 and approaches the range of experimental error inherent in any method of protein estimation.

TL;DR: A modification of the diphenylamine procedure of Giles and Myers (2) is described which has 30% higher sensitivity and a lower reagent blank than the parent procedure, and is more conveniently carried out.

TL;DR: The method of Matsubara and Sasaki (using 6N HCl and thioglycolic acid) gives comparatively low recoveries for tryptophan, while Liu and Chang's method, using p-toluenesulfonic acid and tryptamine, is more suitable.

TL;DR: 2-site IRMA is more economical in antigen, is unlikely to be subject to deleterious allosteric reactions, and has a lower zero dose-response than usual IRMA, and may also have advantages in reagent stability, specificity, antigen protection, and suitability for automation.

TL;DR: Intrinsic optical density, Folin, and Biuret color development have been carefully studied as methods of determining actin concentration in solution and it appears that the Lowry (Folin) method is the most sensitive and reliable method.

TL;DR: A two-dimensional electrophoresis system for analysis of ribosomal proteins with several advantages over previous systems is described, and is relatively simple and inexpensive to construct and use.

TL;DR: The method described in this communication is sensitive and allows direct determination of the specific radioactivities of proline and hydroxyproline in collagen and in noncollagenous proteins, when proline-14C is used as a precursor.

TL;DR: The proposed amyloglucosidase, glucose oxidase: peroxidase method has the advantage of rapidity, whereas the traditional method consisting of extraction, precipitation, and acid hydrolysis is not only time consuming, but may also be subject to losses of glycogen in each step.

TL;DR: Distribution of xanthine oxidase in various rat tissues was measured by this method, and it was shown that the activity was high in the liver, the small intestine, and the spleen and that the formation of uric acid was not influenced by uricase.

TL;DR: A tlc-technique for two-dimensional separation of 24 most common PTH 1 -amino acids obtained after manual or automated Edman degradations is developed and in addition to direct Edman degradation allows correct placing of Gln and Asn within sequences.

TL;DR: Reactivity of peptides with fluorescamine appears to depend mainly on the nature of amino acids occupying the amino termini; ϵ-amino groups of lysine residues in the peptides tested have been found not to contribute significantly in yielding fluorescence in the reaction.

TL;DR: The elution patterns of both cyclic nucleotides through the entire purification procedure are highly reproducible with excellent recovery.

TL;DR: Butyl acetate exhibited the most satisfactory properties for measurements at 310 nm, which is the absorption peak for unreduced PMA, and is among the least toxic of organic solvents.

TL;DR: Different procedures for the extraction of tissue phosphatidic acid, lecithin,osphatidylethanolamine, and phosph atidylserine, as well as their corresponding lyso derivatives, have been compared.

TL;DR: Using refinements of established procedures and a new two buffer gel filtration method, it is possible to prepare human platelet myosin which by gel electrophoresis is free from actin and over 95% pure.

TL;DR: A simple microtechnique has been developed for the accurate determination of DNA in less than 1 × 10 6 cultured human fibroblasts or lymphoblasts by rapid separation of DNA from the cell extract supernatant and from the extraction buffer to avoid interference with the colorimetric diphenylamine assay.

TL;DR: The possibility that the catalases of eukaryotes and prokaryotes may also have been independently and simultaneously evolved in response to the selection pressures imposed by atmospheric oxygen is considered.

TL;DR: The method was easily modified to determine sphingosine in gangliosides by extracting the hydrophobic base from the hydrolysis mixture with ether, and should have broad application in the field of sphingolipid chemistry and biochemistry.

TL;DR: The incorporation of 6 m urea into polyacrylamide-agarose gels reduces the width of the bands formed upon electrophoresis by single-stranded RNA molecules in the 10 5 - to 10 6.5 -dalton size range, which increases the resolving power of gels and molecules that differ in size by as little as 5% can be separated.

TL;DR: Two procedures are described for the isolation of purified DNA from mammalian tissues by using hydroxyapatite chromatography, a modification of the MUP method of Britten et al. in which some technical difficulties are overcome and the yield and the purity of the DNA are improved.

TL;DR: The stoichiometry after hydrolysis of the oxidation of the stable nonfluorescent compound 2′,7′-dichlorofluorescin diacetate (LDADCF) was determined and found to be 2 moles of DCF produced per mole of hydrogen peroxide used.

TL;DR: Values for the amount of tubulin in various rat tissues have been determined by both the charcoal method and by gel filtration on Sephadex G-100, and in all cases the values determined by the two methods correspond closely.