scispace - formally typeset
Search or ask a question

Showing papers in "Analytical Biochemistry in 1980"


Journal ArticleDOI
TL;DR: This approach provides two major advantages compared with other available methods: it uses an exact mathematical model of the ligand-binding system, thereby avoiding the possible biases introduced by several commonly used approximations and it uses a statistically valid, appropriately weighted least-squares curve-fitting algorithm with objective measurement of goodness of fit.

8,717 citations


Journal ArticleDOI
TL;DR: The dissociation of chromatin is critical to accurate determinations of DNA in biological materials using this method, which can detect as little as 10 ng of DNA with rather unsophisticated instrumentation.

4,902 citations


Journal ArticleDOI
TL;DR: It is reported here that 2-vinylpyridine is a much better reagent for the derivitization of glutathione, and it is demonstrated that the total glutATHione concentration in mouse plasma is substantially higher than generally reported and that glutathion disulfide constitutes less than 30% of the totalglutathione present.

4,279 citations


Journal ArticleDOI
TL;DR: The number of steps in the procedure is reduced from 10 to 6, the reagents in each step are simplified, and the amount of silver required is reduced by a factor of 10, thus greatly reducing the expense of the procedure.

3,223 citations


Journal ArticleDOI
TL;DR: A new technique is described for the electrophoretic analysis of plasminogen activators in sodium dodecyl sulfate-polyacrylamide gels containing copolymerized pl asminogen and gelatin, which can be used to detect as little as 1 mU of urokinase and effectively distinguishes between melanoma- and u rokinase-type plasmineg activators.

1,988 citations


Journal ArticleDOI
TL;DR: Phospholipids may be measured colorimetrically (as dipalmitoyl lecithin) without conventional acid digestion and color development procedures by forming a complex with ammonium ferrothiocyanate.

1,673 citations


Journal ArticleDOI
TL;DR: A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathiona disulfide, cysteine glutathion-mixed disulfides and 20 related sulfur-containing amino acids or their derivatives has been described.

1,169 citations


Journal ArticleDOI
TL;DR: It is shown how the method can be used to determine the approximate molecular weight of the DNA topoisomerase polypeptide by sectioning a gel on which a partially pure sample has been fractionated by electrophoresis.

1,142 citations



Journal ArticleDOI
TL;DR: Low activity and linear plots of microsomal protein concentration versus activity are obtained by including bovine serum albumin or ethylenediamine-tetraacetate in the incubation mixture and conditions are described for minimizing the effects of the instability of resorufin and of inhibition by the product.

825 citations


Journal ArticleDOI
TL;DR: A reverse-phese high-performance liquid chromatography procedure was developed for the separation of biopterin from other unconjugated pteridines and a possible role for tetrahydroneopterin as a cofactor in the enzymatic hydroxylation of aromatic amino acids in man is discussed.

Journal ArticleDOI
TL;DR: DNA fragments from 5% polyacrylamide gels were efficiently blotted after 36 h onto nitrocellulose filters using bidirectional transfer and were shown to be efficient substrates for homologous [32P]DNA probes.

Journal ArticleDOI
TL;DR: Five new zwitterionic hydrogen ion buffers are described for the first time, all N-substituted 3-amino-2-hydroxypropanesulfonic acids, which appear to be equivalent to or better than any other buffers heretofore available.

Journal ArticleDOI
TL;DR: Enzyme-linked immunoadsorbant assays have been developed for types I, II, III, and IV collagen and for laminin and fibronectin and offer a specific, sensitive, and convenient method for the measurement of various connective tissue components either separately or simultaneously.

Journal ArticleDOI
TL;DR: The use of difference in absorbance between 235 and 280 nm for determination of protein concentration is reported.

Journal ArticleDOI
TL;DR: Gangliosides that bind cholera toxin can be detected by the direct binding of 125 I-labeled toxin to thin-layer chromatograms followed by autoradiography, which may be useful in defining the receptors of other toxins or proteins such as some lectins, antibodies, and hormones that bind carbohydrates.

Journal ArticleDOI
TL;DR: A new electrophoretic technique for transferring proteins and nucleic acids from either polyacrylamide or agarose slab gels has been developed as an alternative to blotting, and efficient transfer of DNA to both nitrocellulose membrane and diazobenzyloxymethyl cellulose is demonstrated.

Journal ArticleDOI
TL;DR: Comparing this assay to others shows it to have many more advantages; for example, in addition to its increased sensitivity and versatility, it employs compounds not shown to be carcinogenic and that are very soluble in water.

Book ChapterDOI
TL;DR: A review of recent studies on high-performance liquid chromatography (hplc) of proteins in gel permeation, ion exchange, reversed phase, normal phase, and affinity modes is presented in this article.

Journal ArticleDOI
TL;DR: A rapid, simple and versatile method for the extraction from agarose gels of small plasmid molecules and DNA fragments generated by restriction endonucleases, based on the partitioning of nucleic acid molecules into 1-butanol as their quaternary ammonium salts, leaving the neutral agarOSE in the aqueous phase.

Journal ArticleDOI
TL;DR: The results indicate that the adsorptive characteristics of proteins for polystyrene tubes routinely used in solid-phase immunoassays should be taken into consideration when designing and interpreting data obtained with such assays.

Journal ArticleDOI
TL;DR: A method is described for colorimetric determination of citrulline following deproteinization, depending upon the reaction with diacetyl monoxime in the presence of sulfuric and phosphoric acids, with diminished heating time compared with existing procedures.

Journal ArticleDOI
TL;DR: The properties of the metachromatic dye toluidine blue have been utilized to determine colorimetrically the amount of heparin covalently coupled to Sepharose, and the procedure represents a simple assay technique which allows the direct quantitation of heParin in immobilized heparIn preparations.

Journal ArticleDOI
TL;DR: A method for the highly efficient desalting of 50–200 μl volumes of solution containing from 10 ng to 5 mg of protein has been developed and should be especially useful for the removal of unbound ligand from a rapidly dissociating ligand-binding protein complex.

Journal ArticleDOI
TL;DR: A manually operated microtome for the rapid preparation of live tissue slices that operates submerged in an isotonic solution and incorporates an automatic mechanism for the removal of the cut slices which are gently carried by a stream of fluid to a strainer reservoir.

Journal ArticleDOI
TL;DR: The method is quick and sensitive and the color yield of hydroxyproline is not affected by chromatography solvents, so it can be used for the analysis of collagen and collagen peptides in extracts of small tissue samples or biopsies.

Journal ArticleDOI
TL;DR: Results on human sera correlate well with those obtained by the previously described enzymatic method and the chemical colorimetric method.

Journal ArticleDOI
TL;DR: A very rapid and sensitive method for the fluorometric assays of hydrogen peroxide and the peroxidase was established by using 3-( p -hydroxyphenyl)propionic acid as the best of these substrates.

Journal ArticleDOI
TL;DR: The hyaluronate recovered in the assay could be recovered in a 80–110% yield, indicating that the assay gives the correct concentration of this polysaccharide in biological material.