Showing papers in "Analytical Biochemistry in 1984"

TL;DR: A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed.

TL;DR: A rapid, sensitive method has been developed to detect antibody-antigen complexes on "Western blots" by using Tween 20 to separate and blot the antigens onto nitrocellulose.

TL;DR: All amino acids, including proline, are converted quantitatively to phenylthiocarbamyl compounds and these are stable enough to eliminate any need for in-line derivatization, providing results comparable in sensitivity and precision to those obtained by state-of-the-art ion-exchange analyzers.

TL;DR: Methods for the estimation of hydrogen peroxide in acetone extracts using titanium(IV) are likely to overestimate hydrogenperoxide when applied to plant leaves because pigments appear to co-precipitate with the titanium complex and cannot be removed by washing with solvents.

TL;DR: A nonisotopic immunoassay has been developed based on a sensitive detection of europium (III) in water solution using time-resolved fluorometry, using rabbit IgG as a model system.

TL;DR: The nitrite method was found to be the best for this SOD assay kit and modified to give better sensitivity and minimize interference by coexisting protein, a factor which has been previously ignored.

TL;DR: Tissues in which hydroxypyridinium crosslinks were plentiful included all forms of cartilage, bone, dentin, ligament, tendon, fascia, intervertebral disc, lung, gut, cervix, aorta, and vitreous humor.

TL;DR: A simple inexpensive sensitive protease assay was developed using soluble fluorescein isothiocyanate (FITC)-labeled casein, which resembles natural substrates of most proteases.

TL;DR: The labeled products retain their immunoreactivity, as illustrated by experiments in vivo with chelate-conjugated antibody to mouse I-AK antigen.

TL;DR: This fluorescence assay is both sensitive and rapid, and should provide a simple and general method for determination of critical micelle concentration of any detergent.

TL;DR: A fluorimetric assay for the indirect determination of superoxide production during the respiratory burst of stimulated polymorphonuclear leukocytes was described, and was sufficiently sensitive that first-derivative kinetic analysis of the respiratory Burst could be quickly analyzed.

TL;DR: It was essential to prewash commercial azocoll and agitate reaction tubes vigorously to obtain reproducible, linear assays, found that assays of both crude extracts of Bacillus subtilis and commercial preparations of subtilisin BPN' gave linear rates of hydrolysis as a function of protease concentration.

TL;DR: The application of this enzyme assay in a visible spectrophotometer, along with the considerable evidence that a single aromatic hydrocarbon-inducible cytochrome P-450 isozyme is responsible for the catalysis, enhances the utility of this substrate in microsomal monooxygenase assays.

TL;DR: The interfering effect ofbeta-glucosidase, which acts on both agluconic and holosidic bonds, is overcome by the addition of D-glucono-1,5-delta-lactone, a specific inhibitor of beta-glUCosidases.

TL;DR: It was found that individual specific polar groups are not in general crucial to binding or desorption, and variations in structure, other than major loosening, do not influence strongly the pattern of protein-hydroxyapatite interaction.

TL;DR: A sensitive quantitative method has been developed to determine the number of disulfide bonds in peptides and proteins, and is as sensitive and quantitative as the sulfhydryl group assay employing 5,5'-dithiobis(2-nitrobenzoic acid) (Ellman's reagent).

TL;DR: A sensitive and convenient method for the simultaneous determination of D- and L-aspartic acid in amino acid mixtures is described, which can be accurately detected in the presence of a 100-fold excess of L- aspartate.

TL;DR: Low levels of Endo H are sensitive to inactivation by sodium dodecyl sulfate, with considerable activity being lost over 4 h when the unbound detergent concentration exceeds protein by 0.02% (0.2 mg/ml).

TL;DR: It is shown that the O-acetyl esters on free sialic acids are relatively more stable under acid conditions but more labile under basic conditions than similar ester on bound sIALic acids.

TL;DR: O2 inhibits the reduction of the tetrazolium by O2-.

TL;DR: A procedure for the determination of free and total cholesterol in lipid extracts using cholesterol oxidase and peroxidase to catalyze the reaction of H2O2 with o-dianisidine to yield a colored product.

TL;DR: These derivatives are particularly useful for gas chromatographic/mass spectrometric analysis involving the use of stable isotopes and selected ion monitoring, since a peak of sufficient abundance at 57 mass/charge units below the molecular ion was always present.

TL;DR: Application of the procedure to detection of virus-specific RNA in cells persistently infected with mouse hepatitis virus demonstrates high reproducibility of recovered RNA and lack of "masking" with up to a 10-fold excess of starting cell material.

TL;DR: A very simple method to obtain peroxidase, which is both about five times cheaper than the rather expensive commercial preparations and has a significant higher activity, is reported.

TL;DR: This stain proved to be useful for the wide-range detection of simple and complex lipids on thin-layer plates and can stain several classes of lipids, including cholesterol, cholesterol esters, glycerides, phospholipid, ceramides, and neutral and acidic glycosphingolipids.

TL;DR: It was observed that the hemoprotein bands stained more intensely than duplicate sample bands that had been stained only with the Coomassie blue R-250, which allows the indication of hemoproteins in gels even after the peroxidase yield has been significantly depleted by reducing agents.

TL;DR: A method is described by which the growth inhibitory effects of cytotoxic compounds on exponentially growing adherent cell lines can be quantitated, offering the advantage that any adherent cell line can be utilized without the need for tedious cell-counting procedures.

TL;DR: In this paper, a reverse-phase high-performance liquid chromatography was used for the simultaneous determination of retinol, α-tocopherol, lycopene and β-carotene in human plasma, which required 100 to 200 μl of plasma and 16 min of elution time per sample.

TL;DR: A new technique for the detection of antibodies bound to proteins blotted onto nitrocellulose paper was developed, which allows detection of bands containing less than 20 ng of protein.