Showing papers in "Analytical Biochemistry in 1991"

TL;DR: The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally described by Merril et al. was modified to reduce unspecific background staining and increase sensitivity and this improved staining procedure is applied for the routine analysis of complex DNA profiles generated by DNA amplification fingerprinting (DAF).

TL;DR: The percentage recovery of functional activity depended on the respective protein complex studied and was zero for some complexes, but almost quantitative for others, and the recovery of all respiratory chain complexes was almost quantitative.

TL;DR: The introduction of the glycine kinker into fusion proteins allows for the cleavage of the fusion proteins while they are attached to the affinity resin resulting in a single step purification of the recombinant protein.

TL;DR: The biospecific interaction of the immobilized ligand with its biological counterpart is illustrated by the effects on the interaction of immunoglobulins with immobilized Staphylococcus aureus protein A for various amounts of protein A.

TL;DR: It is found that a small amount of sulfamate suppresses color production by a 20-fold excess of some neutral sugars without substantial sacrifice of the sensitive detection of uronic acids by the diphenyl reagent.

TL;DR: The theory of light transport in strongly scattering media as monitored in the time and frequency domains is reviewed, and algorithms for quantitation of hemoglobin saturation from the photon decay rate (delta log R/delta t), and from the phase-shift (theta) obtained from frequency-resolved, phase-modulated spectroscopy, are developed.

TL;DR: A series of fluorescent, long-wavelength, benzo[c]-xanthene dyes has been characterized for pH measurement in both excitation and emission ratio applications and should be suitable for simultaneous determination of pH and Ca2+ transients.

TL;DR: Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and D-glucose by glucose oxidase) have been miniaturized using a microsample plate reader to measure the hydrolysis of starch by alpha-amylase and lactose by lactase.

TL;DR: The comprehensive parameter set determined here greatly extends the ability to analyze homology, membrane directedness, and folding potential of proteins.

TL;DR: The above procedure was applied to the determination of phosphorylase phosphat enzyme activity in bovine brain extracts and the results are comparable to those obtained with the radioisotopic phosphatase assay.

TL;DR: A procedure for the nonradioactive labeling of oligonucleotides with the hapten digoxigenin (DIG) has been developed and the efficiency of the labeling reactions with respect to variation of the different parameters was analyzed.

TL;DR: Successful isolation of nucleic acids from strawberry (Fragaria X ananassa) receptacle, a particularly recalcitrant tissue, and from a range of tissues of other plant species demonstrates the general applicability of the method.

TL;DR: Excellent correlation was observed for the electrophoretic mobilities measured by capillary zone electrophoresis versus q/MW2/3, which suggests that the frictional drag is proportional to the surface area of a sphere that has a volume proportional toThe MW.

TL;DR: The practicability of DNA molecules bound to microwells for hybridization is investigated and Carbodiimide-mediated condensation of DNA onto microwells is investigated.

TL;DR: An optimized chemiluminescent assay for beta-galactosidase using a chemilUMinescent substrate AMPGD using a lacZ-expressing vectors and its use for the quantitation of beta-GalactOSidase in cells transfected with LacZ- expressing vectors is described.

TL;DR: The use of acridine orange, and probably all metachromatic dyes, as probes for monitoring pH gradients is limited, since these probes neither reflect quantitatively the amount of H+ pumped nor the effect of anions and temperature on transmembrane H+ transport.

TL;DR: This method will permit the on-line detection of NO formation from endogenous and pharmacological sources in oxygen-containing aqueous media by means of electron paramagnetic resonance spectroscopy.

TL;DR: Preparative steps led to the detection of a single peak that on spectral analysis was identified as pure MDA-TBA, and this procedure offers several advantages in terms of specificity, recovery, and reproducibility.

TL;DR: The spectroscopic properties of the fatty acid probes and their phospholipid derivatives are particularly suited to applications in fluorescence imaging of cellular lipid distribution and membrane level studies of lateral lipid segregation.

TL;DR: A method for preparative in vitro mRNA synthesis is presented, which makes it possible to generate several hundred RNA copies per molecule of linearized DNA template.

TL;DR: By using the PCR-mediated single-strand conformation polymorphism method, isolating each polymorphic DNA strand, and amplifying it by a second-stage PCR for its sequence determination, the sequence of a minor constituent can be determined accurately.

TL;DR: The vaccinia/T7 transient expression system, which results in rapid, high-level expression of proteins encoded by plasmids bearing T7 promoters, provides a powerful strategy for the expression cloning of membrane transporters.

TL;DR: A radioactive assay for the determination of pyruvate dehydrogenase complex activity in muscle tissue has been developed and is specific and sensitive to the picomole range of acetyl-CoA formed.

TL;DR: The relatively good ir analysis for concanavalin A and alpha-chymotrypsin indicate that the ir method is less affected by the presence of aromatic groups, and the inverse matrix necessary to analyze unknown proteins are presented.

TL;DR: The preparations of N alpha-Fmoc-3-nitro-L-tyrosine and N-Boc-anthranilic acid Dhbt ester and their application to parallel multiple column solid-phase peptide synthesis is described and the importance of long substrates for optimal enzymatic activity was demonstrated.

TL;DR: The structure and purity of each of the eight new commercial heparin/heparan sulfate disaccharide standards were confirmed using fast-atom-bombardment mass spectrometry and high-field 1H-NMR spectroscopy.

TL;DR: Using the single cell eukaryote Tetrahymena thermophila, a simple method was developed for studying protein-DNA associations by cross-linking proteins to DNA with formaldehyde and immunoprecipitating the solubilized chromatin fragments with a specific antiserum.

TL;DR: Four new fluorogenic heptapeptide substrates have been synthesized with sequences that are optimized for five human matrix metalloproteinases (MMP) and characterized with respect to their composition, solubility, optical and fluorescence spectra, and hydrolysis by their target MMP.

TL;DR: A protein kinase C-selective peptide substrate was used to develop a method for measuring PKC activity directly and quantitatively in isolated cell membranes without prior detergent extraction and reconstitution of the enzyme with phosphatidylserine and TPA in the presence of excess Ca2+.