Showing papers in "Analytical Biochemistry in 2009"

TL;DR: A new dynamic kinetic simulation program that allows multiple data sets to be fit simultaneously to a single model based on numerical integration of the rate equations describing the reaction mechanism is described.

TL;DR: The miRNA-based body fluid identification assays were highly specific because the miRNA expression profile for each body fluid was different from that obtained from 21 human tissues.

TL;DR: No gene was identified that exhibited such a constant level of expression as to outperform the other candidates under all experimental conditions, highlighting the need for normalizing gene expression in tomato using the geometric average of multiple carefully selected reference genes.

TL;DR: Confidence contour analysis obtained by measuring the dependence of the sum square error on each pair of parameters while allowing all remaining parameters to be adjusted in seeking the best fit reveals complex relationships between parameters and clearly outline the space over which parameters can vary.

TL;DR: A silver-staining method that can efficiently stain up to 14 gels at one time and with a detection limit of approximately 10 pg of DNA/mm(2), which is comparable to other methods.

TL;DR: In thiol–disulfide exchange reactions, it is important to consider reaction rate and the equilibrium constants between various thiol and disulfide species, because the thiolate anion is the reactive species, these properties are particularly sensitive to thiol pKa values.

TL;DR: Asparagine deamidation in light chain complementarity determining region 1 of a humanized IgG1 monoclonal antibody is identified and both Asp and isoAsp are assigned, which were assigned by Edman degradation and byisoAsp detection using protein isoaspartate methyltransferase.

TL;DR: A kind of nanocomposite with good dispersion in water was prepared through covalent adsorption of ferrocenecarboxaldehyde on multiwalled carbon nanotubes (MWNTs) for electrical communication between glucose oxidase (GOD) and electrode.

TL;DR: Using this 30-min in-solutiontrypsin digestion method, little protocol-induced deamidation or N-terminal glutamine cyclization product was observed and cleaner tryptic maps were obtained due to less trypsin self-digestion and fewer nonspecific cleavages.

TL;DR: A differential method was developed and applied to obtain a reliable set of metabolome data for Escherichia coli K12 MG1655 grown in steady-state, aerobic, glucose-limited chemostat cultures and it was found that direct filtration without cooling of the sample was the most appropriate.

TL;DR: The new predictor, called Hum-mPLoc 2.0, is obtained, where the accession number for input is no longer needed whatsoever and both the functional domain information and the sequential evolution information have been fused into the predictor by an ensemble classifier, so that the prediction power has been significantly enhanced.

TL;DR: Two gold-based lateral-flow strips were investigated for simultaneous detection of carbofuran and triazophos and the portable one-step strip assay showed strength in the simultaneous screening for two pesticides within a short time without any equipment.

TL;DR: The proposed immunosensor, by using GNP/CNT/Ch as the immobilization matrix of AFP, offers an excellent amperometric response of ALP-anti-AFP to 1-NP, and provided a new alternative to the application of other antigens or other bioactive molecules.

TL;DR: A novel method based on the concept of Chou's pseudo amino acid composition has been developed for predicting and recognizing GPCRs, and the prediction accuracies were 99.72%, 97.64%, and 99.20%, showing 9.4% to 18.0% improvement over other existing methods.

TL;DR: 2-aminopyridine was the label with the lowest retention on reversed-phase and graphitic carbon columns and appears to be most suitable for glycan fractionation by multidimensional HPLC, and most glycan derivatives performed better than native sugars in matrix-assisted laser desorption/ionization-mass spectrometry and electrospray ionization-MS.

TL;DR: (monolyso)cardiolipin analysis by HPLC-MS not only is a powerful tool to diagnose patients with clinical signs and symptoms of BTHS but also should be used in patients suffering from mitochondrial myopathies with unknown etiology.

TL;DR: Data indicate that IRDye 800CW 2-DG is a broadly applicable optical imaging agent for in vivo imaging of neoplasms in mice with good signal-to-noise characteristics.

TL;DR: A highly sensitive time-resolved Förster resonance energy transfer (FRET) assay is described for quantification of soluble mutant huntingtin in brain, plasma, and cerebrospinal fluid and in mice, soluble huntingtin levels decrease during disease progression, inversely correlating with brain aggregate load.

TL;DR: The analytical method developed here provides a qualitative and quantitative gas chromatography/mass spectrometry (GC/MS) analysis, with high sensitivity and efficiency, coupled with derivatization of ethyl chloroformate in aqueous medium, showing a promising application in metabolome analysis based on human fecal water samples.

TL;DR: A complete set of phospho-antibodies against the three known phosphorylation sites on the E1 alpha subunit of pyruvate dehydrogenase (PDHE1alpha) are developed, demonstrating that these reagents should be useful for monitoringosphorylation of PDHE1 alpha and, therefore, overall metabolism in the disease state as well as in response to a myriad of physiological and pharmacological stimuli.

TL;DR: Good correlation of test results for 68 patient serum samples with a commercially available reference method (LiBASys assay for AFP-L3%) was obtained, with r(2)=0.981 and slope=1.03.

TL;DR: The quality, enrichment, and purity of mitochondria isolated with this protocol are comparable to mitochondria obtained using the ultracentrifuge method, and a typical separation procedure takes only approximately 1 to 2h from initial cell homogenization.

TL;DR: This assay is applicable to any nicotinamide-forming enzyme and will be an important tool to address many outstanding questions surrounding their regulation and is readily adaptable to high-throughput screening.

TL;DR: It is suggested that OMC is a promising material in the detection of thiols in biologically relevant experimental conditions (in terms of pH) and in the physiological levels of GSH and CySH.

TL;DR: An amperometric lactate biosensor developed based on a conducting polymer, poly-5,2'-5',2''-terthiophene-3'-carboxylic acid, and multiwall carbon nanotube (MWNT) composite on a gold electrode demonstrated successfully for the analysis of l-lactate concentration in commercial milk and human serum samples.

TL;DR: Differences in diurnal hepcidin patterns were observed between urine and serum, and high serum ferritin levels were associated with higher serum hePCidin levels but not with urine levels.

TL;DR: Glycoproteins remodeled with glycosyltransferases and glycosidases are used for testing specificities of lectins from Aleuria aurantia, Erythrina cristagalli, Griffonia simplicifolia, and wheat germ as well as reactivities of anti-carbohydrate antibodies.

TL;DR: Critically evaluating popular error-prone polymerase chain reaction (PCR) protocols as well as hydroxylamine and a mutator Escherichia coli strain mutagenesis methods yielded the highest mutation rates and the widest operational ranges, whereas the chemical and biological methods generated a low level of mutations and exhibited a narrow range of operation.

TL;DR: This technique allows linear relative quantitation of glycans over a 10-fold concentration range and can accurately quantify sub-picomole levels of released glycans, providing a needed advancement in the field of glycomics.

TL;DR: The E-AuNPs modified electrode catalyzes the oxidation of AA andUA, separates their voltammetric signals by 200mV, and has excellent sensitivity towards AA and UA with a detection limit of 50nM.