Showing papers in "Analytical Biochemistry in 2016"

TL;DR: By incorporating the sequence-coupling effects into the general pseudo amino acid composition and using KNNC (K-nearest neighbors cleaning) treatment and IHTS (inserting hypothetical training samples) treatment to optimize the training dataset, a predictor called iSuc-PseOpt has been developed andorous cross-validations indicated that it remarkably outperformed the existing method.

TL;DR: A new predictor called pRNAm-PC is proposed, in which RNA sequence samples are expressed by a novel mode of pseudo dinucleotide composition (PseDNC) whose components were derived from a physical-chemical matrix via a series of auto-covariance and cross covariance transformations.

TL;DR: This electrode can effectively analyze glucose concentration in human serum samples, avoiding interference, and is a promising non-enzymatic glucose sensor due to its low overpotential, high sensitivity, good selectivity and stability, fast response, and low cost.

TL;DR: Simulations demonstrate the limits of KD that can be measured in current instrumentation and show that binding kinetics is a major concern in planning and executing MST experiments, and indicate a set of best practices for performing and analyzing M ST experiments.

TL;DR: The applicability of sensor for the analysis of DA, and UA in dopamine injection solution, human serum and human urine samples was successfully demonstrated.

TL;DR: Using the secreted Transforming Growth Factor-β (TGF-β) family ligand BMP-4 and its receptors as example, surface plasmon resonance can be used to accurately determine IC50 values of individual ligand-receptor pairings.

TL;DR: It is found that Ponceau staining optimally balanced accuracy and precision, and it is suggested that this approach be considered as an alternative to normalizing with a high-abundance protein.

TL;DR: The results support the need for a "fit-for-purpose" approach in instrument selection for biosensor studies and find that despite certain inherent systematic limitations in instrumentation, the rank orders of both the association and dissociation rate constants were highly correlated between these instruments.

TL;DR: Comparison between 49-mer and other shorter aptamers showed that shortening aptamer sequences through removing excess flanking nucleotides outsides of binding region improved colorimetric sensitivity for acetamiprid by 3.3 fold.

TL;DR: Basic strategies and recent developments for the enhancement of the sensory performance of nanostructures in the electroanalysis of pharmaceuticals are reviewed and their application as modified electrodes for drug assays is presented.

TL;DR: The results indicated that GO-SELEX technology was appropriate for the screening of aptamers against small-molecule toxins, offering a promising application for aptamer-based biosensors.

TL;DR: This colorimetric method was exploited to detect bisphenol A based on BPA-specific aptamer and cationic polymer-induced aggregation of gold nanoparticles (AuNPs) and was successfully used to determine spiked BPA in tap water and river water samples.

TL;DR: Electroanalytical methods are attracting much attention because of their advantage over conventional methods, as they are fast, simple, sensitive, and cost effective.

TL;DR: The use of an immobilized bacteriophage is reported for the development of a highly sensitive electrochemical sensor for Staphylococcus arlettae, bacteria from the pathogenic family of coagulase-negative staphylitis (CNS).

TL;DR: A new sensitive electrochemical sensor, a glassy carbon electrode modified with chemically cross-linked copper-complexed chitosan/multiwalled carbon nanotubes (Cu-CS/MWCNT/GCE) showed good selectivity, stability, and reproducibility and was used to determine the presence of rutin in fruits with satisfactory results.

TL;DR: It is found that sucrose not only could increase the rate of protein recovery after 1 h acetone precipitation, but also did not interfere with Bradford assay, so a method for rapid protein quantitation in protein drugs even if they contained interfering substances is developed.

TL;DR: This paper reports a label-free electrochemical immunosensor for the determination of aflatoxin B1 (AFB1), which is based on a gold electrode modified by a biocompatible film of carbon nanotubes/poly(diallyldimethylammoniumchloride)/Pd-Au nanoparticles (CNTs/PDDA/Pd

TL;DR: A particular advantage of the new approach described here is that the short run times (5-10 min) reduce opportunities for dissociation of bound complexes, an important concern in non-equilibrium nucleic acid binding experiments.

TL;DR: The proposed immunosensor was used to determine CEA in clinical serum samples with satisfactory results and may provide promising potential application in clinical immunoassays with the properties of facile procedure, stability, high sensitivity, and selectivity.

TL;DR: The immobilized uricase onto graphene oxide (GO) and its function for electrochemical detection of uric acid and its exhibited a high selectivity towards UA in the presence of other interference as well as good reproducibility.

TL;DR: A reliable and reproducible method for embedding spheroids using the hanging-drop/agarose method within collagen is described herein and can be used to guide experimental manipulation of 3D cell cultures and to evaluate anticancer drug efficacy.

TL;DR: EB was found to be an effective dye for accurate counting of cells with different viabilities across three different automated cell counters and was less toxic to cultured HL-60 cells during the cell counting process.

TL;DR: RNA-MethylPred, a new bioinformatics model developed by incorporating bi-profile Bayes, dinucleotide composition, and k nearest neighbor (KNN) scores for three feature extractions, demonstrated that it might be a powerful and complementary tool for further experimental investigation of N6-methyladenosine modification.

TL;DR: This study successfully developed an analytical method capable to simultaneously quantify urinary 8-OHdG,8-NO2Gua, 8-IsoPGF2α, and HNE-MA, which can be applied towards conducting epidemiological studies to reveal the mechanisms related to disease development, and thus evaluate the associated risks of diseases.

TL;DR: Palladium nanoparticles were potentiostatically electrodeposited on a gold surface at a highly negative potential to immobilize a Brucella-specific probe and the process of immobilization and hybridization was detected by electrochemical methods.

TL;DR: Evidence is presented that Ponceau S is more reliable than housekeeping protein levels for specific protein quantifications in Western blot analysis.

TL;DR: The experimental results show that TOCNCs are suitable material for chiral sensor and the contrast of serum sample of healthy people and patients with type 2 diabetes also was proposed, and significant difference was exhibited on the modified electrode.

TL;DR: A single-step assay for measuring LacZ activity is described using a 96-well microplate reader with a proven alternative cell permeabilization method that reduces handling time by 90%.

TL;DR: The present study demonstrates that the fabricated FITC-FA-BSA-SPIO NPs hold promising perspectives by providing a dual-modal imaging as non-toxic and target-specific vehicles in human brain tumor treatment in future.