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Showing papers in "Analytical Biochemistry in 2017"


Journal ArticleDOI
TL;DR: Additional potential mechanisms for which ΔΨm is essential for maintenance of cellular health and viability are proposed and recommendations how to accurately measure ΔΩm in a cell are provided and potential sources of artifacts are discussed.

1,024 citations


Journal ArticleDOI
TL;DR: In this paper, the authors reviewed reported analytical methods and their principles for the quantitative measurement of MDA, HNE and 15(S)-8-iso-PGF2α in biological samples including plasma and urine, and critically discusses their biological and biomedical outcome which is rarely crystal clear and free of artefacts.

978 citations


Journal ArticleDOI
TL;DR: Healthy human brain glutathione concentration is ∼1-2 mM, but it varies by brain region, with evidence of gender differences and age effects, and improved methods to measure glutATHione in vivo will provide better precision in glutathion determination and help resolve the complex biochemistry of this molecule in health and disease.

116 citations


Journal ArticleDOI
TL;DR: Performances, drawbacks and advantages of different methods of SCFAs analysis in feces are discussed, especially in the light of choosing a proper pretreatment, as feces is a complex biological material.

100 citations


Journal ArticleDOI
TL;DR: How 1H MRS allows the measurement of Cr and PCr, and how 31P MRS makes it possible to estimate the creatine kinase (CK) rate constant and so detect dynamic changes in the Cr/PCr/CK system are described.

87 citations


Journal ArticleDOI
TL;DR: The results demonstrated that the GOx/PVA-Fe3O4/Sn bioelectrode is promising in biosensor construction and has good anti-interfering ability during the glucose detection, fast response, good reproducibility and satisfactory stability.

85 citations


Journal ArticleDOI
TL;DR: Moving forward, the combination of advanced separation and ion activation techniques is likely to provide mass spectrometry-based lipidomics with its best opportunity to achieve complete molecular-level lipid characterization and measurement from complex mixtures.

79 citations


Journal ArticleDOI
TL;DR: Such an aptasensor opens a rapid, selective and sensitive route for HCV core antigen detection and provides a promising strategy for potential applications in clinical diagnostics.

78 citations


Journal ArticleDOI
TL;DR: The use of more accurate redox-specific extinction coefficients and method calculations will be an invaluable tool for both in vitro and in-vivo experiments aimed at determining the role of Hb-mediated vascular pathology in hemolytic anemias and when Hb is used as oxygen therapeutics.

76 citations


Journal ArticleDOI
TL;DR: According to the analysis of potential interferences, the proposed sensor was specifically detecting parathion even in the presence of its metabolite, paraoxon, which supported its potential practical applicability.

76 citations


Journal ArticleDOI
TL;DR: A methodological overview of the 31P-MR techniques that can be used for basic, physiological, or clinical research of human skeletal muscle and liver in vivo is presented.

Journal ArticleDOI
TL;DR: The relevance of B0 homogeneity for in vivo MRS is described and common concepts and specific solutions for its experimental optimization are summarized.

Journal ArticleDOI
TL;DR: A novel nanocomposite based on the graphene quantum dots and CoNiAl-layered double-hydroxide was successfully synthesized by co-precipitation method and used as a modifier to fabricate a modified carbon paste electrode as a non-enzymatic sensor for glucose determination.

Journal ArticleDOI
TL;DR: This biosensor measured creatinine level in sera of apparently healthy subjects and persons suffering from renal and muscular dysfunction, and lost 10% of its initial activity within 240 days of its regular uses, when stored at 4 °C.

Journal ArticleDOI
Vishal Kamat1, Ashique Rafique1
TL;DR: The data presented in this study suggest that under optimal experimental conditions, Octet biosensor is capable of generating kinetic values comparable to SPR/SPRi biosensors.

Journal ArticleDOI
TL;DR: Aptamer-based fluorescence sensors from a truncated progesterone (P4) aptamer, highly selective for P4 against similar compounds such as 17-β estradiol, bisphenol-A and vitamin D are designed and applied in food, pharmaceutical, and clinical industries.

Journal ArticleDOI
TL;DR: An approach that utilizes structural features of amino acids to improve lysine succinylation prediction, referred to as SucStruct (Succinylation using Structural features), proved to significantly improve performance when compared to previous predictors, with sensitivity, accuracy and Mathew's correlation coefficient significantly improved.

Journal ArticleDOI
TL;DR: The combination of metabolite profiling and physiological parameters assisted to understand precisely the mechanisms of plant response at volatile metabolome level in drought sensitive and tolerant thyme plant populations.

Journal ArticleDOI
TL;DR: Fundamental concepts and methods for pooled CRISPR screens are presented and biological results from recent genome-scale loss-of-function and gain- of-function screens are reviewed.

Journal ArticleDOI
TL;DR: The Fe3O4@Au-based pseudo-homogeneous electrochemical immunosensor exhibited higher anti-interference ability, acceptable reproducibility and long-term stability for AFP detection.

Journal ArticleDOI
TL;DR: It is shown that low micromolar levels of copper compounds such as Cu(II)Urea2, Cu( II)Ser2 and CuCl2 can interfere with the MTT assay making improper the detection of formazan product of MTT reduction.

Journal ArticleDOI
TL;DR: It was shown that the new DNA aptamer sequence has the ability to use for detection and capture of E. coli O157 and could be used for detection of the related food borne diseases.

Journal ArticleDOI
TL;DR: Carbon quantum dots have been proposed as an effective platform for miRNA detection and the quenching of the dots fluorescence due to fluorescence resonance energy transfer (FRET) was used for mir 9-1 detection.

Journal ArticleDOI
TL;DR: The main objective of this paper is to serve as a narrow footbridge by comparing the literary works on the β-galactosidase biosensors, critically analyze their use in the construction of best biosensor by showing the pros and cons of the predicted methods for the practical use of bios Sensors.

Journal ArticleDOI
TL;DR: In this review, recent advances and applications of aptasensors for the detection and quantification of S. typhimurium in details have been summarized.

Journal ArticleDOI
TL;DR: A formulation for synthesizing silica-PAMAM dendrimer hybrid nanoparticles with sol-gel technique is developed to develop a formulation in terms of inhibiting the proliferative effects of neuroblastoma and the inhibitory effect was associated with the anthocyanins.

Journal ArticleDOI
TL;DR: Overall, data suggested that the developed biosensor holds great promise as a useful alternative diagnostic tool for the detection of different cancer biomarkers, in particular PSA present in biological samples.

Journal ArticleDOI
TL;DR: Compared with free Fe3O4 MNPs and BSA-Au nanoclusters, the composites have better catalytic activity at higher temperature and lower pH, indicating that Fe 3O4@SiO2@Au MNPs can work in more severe environment.

Journal ArticleDOI
TL;DR: Results showed that the proposed materials exhibited enhanced characteristics, e.g., low limits of detection, wide linear ranges, low adsorption, and low background current, which make them promising candidates for electrochemical sensing applications.

Journal ArticleDOI
TL;DR: The methodological aspects of detecting low-abundant J-coupled metabolites via 1D spectral editing techniques and 2D nuclear magnetic resonance (NMR) methods applied in vivo, in humans, with a focus on the brain are reviewed.