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Showing papers in "Applied Optics in 2006"


Journal ArticleDOI
TL;DR: The foundations of passive imaging polarimetry, the phenomenological reasons for designing a polarimetric sensor, and the primary architectures that have been exploited for developing imaging polarimeters are discussed.
Abstract: Imaging polarimetry has emerged over the past three decades as a powerful tool to enhance the information available in a variety of remote sensing applications. We discuss the foundations of passive imaging polarimetry, the phenomenological reasons for designing a polarimetric sensor, and the primary architectures that have been exploited for developing imaging polarimeters. Considerations on imaging polarimeters such as calibration, optimization, and error performance are also discussed. We review many important sources and examples from the scientific literature.

1,374 citations


Journal Article
TL;DR: This is the first volume of a set of three within the Springer Series in Optical Sciences, and is devoted to photorefractive effects, photoreFractive materials, and their applications.
Abstract: This is the first volume of a set of three within the Springer Series in Optical Sciences, and is devoted to photorefractive effects, photorefractive materials, and their applications. Since the publication of our first two Springer books on Photorefractive Materials and Their Applications (Topics in Applied Physics, Vols. 61 and 62) almost 20 years ago, a lot of research has been done in this area. New and often expected effects have been discovered, theoretical models developed, known effects finally explained, and novel applications proposed. We believe that the field has now reached a high level of maturity, even if research continues in all areas mentioned above and with new discoveries arriving quite regularly. We therefore have decided to invite some of the top experts in the field to put together the state of the art in their respective fields. This after we had been encouraged to do so for more than ten years by the publisher, due to the fact that the former volumes were long out of print.

1,154 citations


Journal ArticleDOI
TL;DR: The state of the art of digital in-line holographic microscopy with numerical reconstruction is reviewed and some technical issues, such as lateral and depth resolution, depth of field, twin image, four-dimensional tracking, and reconstruction algorithm are discussed.
Abstract: We first briefly review the state of the art of digital in-line holographic microscopy (DIHM) with numerical reconstruction and then discuss some technical issues, such as lateral and depth resolution, depth of field, twin image, four-dimensional tracking, and reconstruction algorithm. We then present a host of examples from microfluidics and biology of tracking the motion of spheres, algae, and bacteria. Finally, we introduce an underwater version of DIHM that is suitable for in situ studies in an ocean environment that show the motion of various plankton species.

534 citations


Journal ArticleDOI
TL;DR: A vector version of the 6S (Second Simulation of a Satellite Signal in the Solar Spectrum) radiative transfer code (6SV1), which enables accounting for radiation polarization, has been developed and validated against a Monte Carlo code, Coulson's tabulated values, and MOBY water-leaving reflectance measurements.
Abstract: A vector version of the 6S (Second Simulation of a Satellite Signal in the Solar Spectrum) radiative transfer code (6SV1), which enables accounting for radiation polarization, has been developed and validated against a Monte Carlo code, Coulson's tabulated values, and MOBY (Marine Optical Buoy System) water-leaving reflectance measurements. The developed code was also tested against the scalar codes SHARM, DISORT, and MODTRAN to evaluate its performance in scalar mode and the influence of polarization. The obtained results have shown a good agreement of 0.7% in comparison with the Monte Carlo code, 0.2% for Coulson's tabulated values, and 0.001-0.002 for the 400-550 nm region for the MOBY reflectances. Ignoring the effects of polarization led to large errors in calculated top-of-atmosphere reflectances: more than 10% for a molecular atmosphere and up to 5% for an aerosol atmosphere. This new version of 6S is intended to replace the previous scalar version used for calculation of lookup tables in the MODIS (Moderate Resolution Imaging Spectroradiometer) atmospheric correction algorithm.

501 citations


Journal ArticleDOI
TL;DR: It is demonstrated that digital holographic microscopy can resolve the locations of several thousand particles and can measure their motions and trajectories using cinematographic holography, and a sample trajectory and detailed morphological information of a free-swimming copepod nauplius are presented.
Abstract: Better understanding of particle-particle and particle-fluid interactions requires accurate 3D measurements of particle distributions and motions. We introduce the application of in-line digital holographic microscopy as a viable tool for measuring distributions of dense micrometer (3.2 microm) and submicrometer (0.75 microm) particles in a liquid solution with large depths of 1-10 mm. By recording a magnified hologram, we obtain a depth of field of approximately 1000 times the object diameter and a reduced depth of focus of approximately 10 particle diameters, both representing substantial improvements compared to a conventional microscope and in-line holography. Quantitative information on depth of field, depth of focus, and axial resolution is provided. We demonstrate that digital holographic microscopy can resolve the locations of several thousand particles and can measure their motions and trajectories using cinematographic holography. A sample trajectory and detailed morphological information of a free-swimming copepod nauplius are presented.

440 citations


Journal ArticleDOI
TL;DR: A procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram, which enables one to reconstruct correct and accurate phase distributions, even in the presence of strong and high-order aberration.
Abstract: We present a procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram. The phase-mask parameters are computed automatically without knowledge of physical values such as wave vectors, focal lengths, or distances. This method enables one to reconstruct correct and accurate phase distributions, even in the presence of strong and high-order aberrations. Examples of applications are shown for microlens imaging and for compensating for the deformations associated with a tilted thick plate. Finally we show that this method allows compensation for the curvature of the specimen, revealing its surface defects and roughness. Examples of applications are shown for microlenses and metallic sphere imaging.

404 citations


Journal ArticleDOI
TL;DR: A flexible and fast Monte Carlo-based model of diffuse reflectance has been developed for the extraction of the absorption and scattering properties of turbid media, such as human tissues, and it was found that optical properties could be extracted from the experimentally measured diffuse reflectances spectra with an average error of 3% or less.
Abstract: A flexible and fast Monte Carlo-based model of diffuse reflectance has been developed for the extraction of the absorption and scattering properties of turbid media, such as human tissues. This method is valid for a wide range of optical properties and is easily adaptable to existing probe geometries, provided a single phantom calibration measurement is made. A condensed Monte Carlo method was used to speed up the forward simulations. This model was validated by use of two sets of liquid-tissue phantoms containing Nigrosin or hemoglobin as absorbers and polystyrene spheres as scatterers. The phantoms had a wide range of absorption (0-20 cm(-1)) and reduced scattering coefficients (7-33 cm(-1)). Mie theory and a spectrophotometer were used to determine the absorption and reduced scattering coefficients of the phantoms. The diffuse reflectance spectra of the phantoms were measured over a wavelength range of 350-850 nm. It was found that optical properties could be extracted from the experimentally measured diffuse reflectance spectra with an average error of 3% or less for phantoms containing hemoglobin and 12% or less for phantoms containing Nigrosin.

322 citations


Journal ArticleDOI
TL;DR: The effects of the nonideal performance parameters of commercial diode lasers are especially important away from the line center of discrete spectra, and these contributions become more pronounced for 2f signals with the large modulation depths needed for WMS at elevated pressures.
Abstract: Tunable diode laser absorption measurements at high pressures by use of wavelength-modulation spectroscopy (WMS) require large modulation depths for optimum detection of molecular absorption spectra blended by collisional broadening or dense spacing of the rovibrational transitions. Diode lasers have a large and nonlinear intensity modulation when the wavelength is modulated over a large range by injection-current tuning. In addition to this intensity modulation, other laser performance parameters are measured, including the phase shift between the frequency modulation and the intensity modulation. Following published theory, these parameters are incorporated into an improved model of the WMS signal. The influence of these nonideal laser effects is investigated by means of wavelength-scanned WMS measurements as a function of bath gas pressure on rovibrational transitions of water vapor near 1388 nm. Lock-in detection of the magnitude of the 2f signal is performed to remove the dependence on detection phase. We find good agreement between measurements and the improved model developed for the 2f component of the WMS signal. The effects of the nonideal performance parameters of commercial diode lasers are especially important away from the line center of discrete spectra, and these contributions become more pronounced for 2f signals with the large modulation depths needed for WMS at elevated pressures.

298 citations


Journal ArticleDOI
TL;DR: A simple iterative algorithm is described to recover the distribution of optical absorption coefficients from the image of the absorbed optical energy, which incorporates a diffusion-based finite-element model of light transport.
Abstract: Photoacoustic imaging is a noninvasive biomedical imaging modality for visualizing the internal structure and function of soft tissues. Conventionally, an image proportional to the absorbed optical energy is reconstructed from measurements of light-induced acoustic emissions. We describe a simple iterative algorithm to recover the distribution of optical absorption coefficients from the image of the absorbed optical energy. The algorithm, which incorporates a diffusion-based finite-element model of light transport, converges quickly onto an accurate estimate of the distribution of absolute absorption coefficients. Two-dimensional examples with physiologically realistic optical properties are shown. The ability to recover optical properties (which directly reflect tissue physiology) could enhance photoacoustic imaging techniques, particularly methods based on spectroscopic analysis of chromophores.

273 citations


Journal ArticleDOI
Fabin Shen1, Anbo Wang1
TL;DR: The implementation of a fast-Fourier-transform based direct integration method is presented, and Simpson's rule is used to improve the calculation accuracy, and the Rayleigh-Sommerfeld diffraction integral integral is investigated.
Abstract: The numerical calculation of the Rayleigh-Sommerfeld diffraction integral is investigated. The implementation of a fast-Fourier-transform (FFT) based direct integration (FFT-DI) method is presented, and Simpson's rule is used to improve the calculation accuracy. The sampling interval, the size of the computation window, and their influence on numerical accuracy and on computational complexity are discussed for the FFT-DI and the FFT-based angular spectrum (FFT-AS) methods. The performance of the FFT-DI method is verified by numerical simulation and compared with that of the FFT-AS method.

263 citations


Journal ArticleDOI
TL;DR: A new three-step phase-shifting algorithm, which is much faster than the traditional three- step algorithm, is proposed, by using a simple intensity ratio function to replace the arctangent function in the traditional algorithm.
Abstract: We propose a new three-step phase-shifting algorithm, which is much faster than the traditional three-step algorithm. We achieve the speed advantage by using a simple intensity ratio function to replace the arctangent function in the traditional algorithm. The phase error caused by this new algorithm is compensated for by use of a look-up-table (LUT). Our experimental results show that both the new algorithm and the traditional algorithm generate similar results, but the new algorithm is 3.4 times faster. By implementing this new algorithm in a high-resolution, real-time 3D shape measurement system, we were able to achieve a measurement speed of 40 frames per second (fps) at a resolution of 532 × 500 pixels, all with an ordinary personal computer.

Journal ArticleDOI
TL;DR: Offering nanometer-scale spatial resolution and real-time reconfigurability, holographic optical traps provide unsurpassed access to the microscopic world and have found applications in fundamental research, manufacturing, and materials processing.
Abstract: Holographic optical tweezers use computer-generated holograms to create arbitrary three-dimensional configurations of single-beam optical traps that are useful for capturing, moving, and transforming mesoscopic objects. Through a combination of beam-splitting, mode-forming, and adaptive wavefront correction, holographic traps can exert precisely specified and characterized forces and torques on objects ranging in size from a few nanometers to hundreds of micrometers. Offering nanometer-scale spatial resolution and real-time reconfigurability, holographic optical traps provide unsurpassed access to the microscopic world and have found applications in fundamental research, manufacturing, and materials processing.

Journal ArticleDOI
TL;DR: The capability of imaging nonspherical lenses without any modification of the optomechanical setup is a key advantage of DHM compared with conventional measurement tools and is demonstrated on a cylindrical microlens and a square lens array.
Abstract: We demonstrate the use of digital holographic microscopy (DHM) as a metrological tool in micro-optics testing Measurement principles are compared with those performed with Twyman-Green, Mach-Zehnder, and white-light interferometers Measurements performed on refractive microlenses with reflection DHM are compared with measurements performed with standard interferometers Key features of DHM such as digital focusing, measurement of shape differences with respect to a perfect model, surface roughness measurements, and optical performance evaluation are discussed The capability of imaging nonspherical lenses without any modification of the optomechanical setup is a key advantage of DHM compared with conventional measurement tools and is demonstrated on a cylindrical microlens and a square lens array

Journal ArticleDOI
TL;DR: A simple, practical method has been developed to correct a spectroradiometer's response for measurement errors arising from the instrument's spectral stray light, fast enough to be integrated into an instrument's software to perform real-time corrections with minimal effect on acquisition speed.
Abstract: A simple, practical method has been developed to correct a spectroradiometer's response for measurement errors arising from the instrument's spectral stray light. By characterizing the instrument's response to a set of monochromatic laser sources that cover the instrument's spectral range, one obtains a spectral stray light signal distribution matrix that quantifies the magnitude of the spectral stray light signal within the instrument. By use of these data, a spectral stray light correction matrix is derived and the instrument's response can be corrected with a simple matrix multiplication. The method has been implemented and validated with a commercial CCD-array spectrograph. Spectral stray light errors after the correction was applied were reduced by 1-2 orders of magnitude to a level of approximately 10(-5) for a broadband source measurement, equivalent to less than one count of the 15-bit-resolution instrument. This method is fast enough to be integrated into an instrument's software to perform real-time corrections with minimal effect on acquisition speed. Using instruments that have been corrected for spectral stray light, we expect significant reductions in overall measurement uncertainties in many applications in which spectrometers are commonly used, including radiometry, colorimetry, photometry, and biotechnology.

Journal ArticleDOI
TL;DR: First-order design of light sources consisting of multiple light-emitting diodes (LEDs) to uniformly illuminate a near target plane is analyzed by considering each single LED as an imperfect Lambertian emitter to achieve uniform near-field irradiance.
Abstract: We analyze the first-order design of light sources consisting of multiple light-emitting diodes (LEDs) to uniformly illuminate a near target plane by considering each single LED as an imperfect Lambertian emitter Simple approximate equations and formulas are derived for the optimum LED-to-LED spacing, ie, the optimum packaging density, of several array configurations to achieve uniform near-field irradiance

Journal ArticleDOI
TL;DR: The authors' experiments with NaCl, clean open ocean seawater, and artificial seawater support the hypothesis that salts modify the absorption spectra of seawater by modifying the molecular matrix and vibrations of pure water.
Abstract: The temperature and salt dependencies of absorption by liquid water (H2O) and heavy water (D2O) were determined using a hyperspectral absorption and attenuation meter (WET Labs, AC-S). Sodium chloride (NaCl) was used as a proxy for seawater salts. There was no significant temperature (ΨT) or salt (ΨS) dependency of absorption at wavelengths <550 nm. At wavelengths >550 nm, ΨT exhibited peaks at ∼604, 662, and 740 nm. A small negative trough in ΨS occurred at ∼590 nm, followed by a small positive peak ∼620 nm, a larger negative trough at ∼720 nm, and a strong positive peak at ∼755 nm. The salt dependency of absorption by heavy water, ΨsH, exhibited a negative power-law shape with very low ΨsH, at wavelengths >550 nm. Our experiments with NaCl, clean open ocean seawater, and artificial seawater support the hypothesis that salts modify the absorption spectra of seawater by modifying the molecular matrix and vibrations of pure water.

Journal ArticleDOI
TL;DR: A system based on digital holographic interferometry for the measurement of vibrations is presented and by combination of the deformations recorded at different times it is possible to reconstruct the vibration of the object.
Abstract: A system based on digital holographic interferometry for the measurement of vibrations is presented. A high-power continuous laser (10 W) and a high-speed CCD camera are used. Hundreds of holograms of an object that has been subjected to dynamic deformation are recorded. The acquisition speed and the time of exposure of the detector are determined by the vibration frequency. Two methods are presented for triggering the camera in order to acquire at a given phase of the vibration. The phase of the wavefront is calculated from the recorded holograms by use of a two-dimensional digital Fourier-transform method. The deformation of the object is obtained from the phase. By combination of the deformations recorded at different times it is possible to reconstruct the vibration of the object.

Journal ArticleDOI
TL;DR: Analysis of transverse and longitudinal intensity distributions of a volume speckle field for the SBMIR technique is presented and enhancement of the resolution method by shifting the camera a distance of a half-pixel in the lateral direction improves the sampling of Speckle patterns and leads to better quality reconstructions.
Abstract: The recording of the volume speckle field from an object at different planes combined with the wave propagation equation allows the reconstruction of the wavefront phase and amplitude without requiring a reference wave. The main advantage of this single-beam multiple-intensity reconstruction (SBMIR) technique is the simple experimental setup because no reference wave is required as in the case of holography. The phase retrieval technique is applied to the investigation of diffusely transmitting and reflecting objects. The effects of different parameters on the quality of reconstructions are investigated by simulation and experiment. Significant enhancements of the reconstructions are observed when the number of intensity measurements is 15 or more and the sequential measurement distance is 0.5 mm or larger. Performing two iterations during the reconstruction process using the calculated phase also leads to better reconstruction. The results from computer simulations confirm the experiments. Analysis of transverse and longitudinal intensity distributions of a volume speckle field for the SBMIR technique is presented. Enhancing the resolution method by shifting the camera a distance of a half-pixel in the lateral direction improves the sampling of speckle patterns and leads to better quality reconstructions. This allows the possibility of recording wave fields from larger test objects.

Journal ArticleDOI
TL;DR: This work describes what it believes to be a new laser-based facility for spectral irradiance and radiance responsivity calibrations using uniform sources (SIRCUS) that was developed to calibrate instruments directly in irradiance or radiance mode with uncertainties approaching or exceeding those available for spectral power responsivities calibrations.
Abstract: Detectors have historically been calibrated for spectral power responsivity at the National Institute of Standards and Technology by using a lamp-monochromator system to tune the wavelength of the excitation source. Silicon detectors can be calibrated in the visible spectral region with combined standard uncertainties at the 0.1% level. However, uncertainties increase dramatically when measuring an instrument's spectral irradiance or radiance responsivity. We describe what we believe to be a new laser-based facility for spectral irradiance and radiance responsivity calibrations using uniform sources (SIRCUS) that was developed to calibrate instruments directly in irradiance or radiance mode with uncertainties approaching or exceeding those available for spectral power responsivity calibrations. In SIRCUS, the emission from high-power, tunable lasers is introduced into an integrating sphere using optical fibers, producing uniform, quasi-Lambertian, high-radiant-flux sources. Reference standard irradiance detectors, calibrated directly against national primary standards for spectral power responsivity and aperture area measurement, are used to determine the irradiance at a reference plane. Knowing the measurement geometry, the source radiance can be readily determined as well. The radiometric properties of the SIRCUS source coupled with state-of-the-art transfer standard radiometers whose responses are directly traceable to primary national radiometric scales result in typical combined standard uncertainties in irradiance and radiance responsivity calibrations of less than 0.1%. The details of the facility and its effect on primary national radiometric scales are discussed.

Journal ArticleDOI
TL;DR: The attenuation of solar radiation in snow can be used to infer the spectral absorption coefficient of pure ice, by reference to a known value at 600 nm, which is lower than even the lowest values of the Antarctic Muon and Neutrino Detector Array experiment on glacier ice.
Abstract: Snow is a scattering-dominated medium whose scattering is independent of wavelength at 350-600 nm. The attenuation of solar radiation in snow can be used to infer the spectral absorption coefficient of pure ice, by reference to a known value at 600 nm. The method is applied to clean Antarctic snow; the absorption minimum is at 390 nm, and the inferred absorption coefficient is lower than even the lowest values of the Antarctic Muon and Neutrino Detector Array (AMANDA) experiment on glacier ice: The absorption length is at least 700 m, by comparison with 240 m for AMANDA and 10 m from laboratory attenuation measurements.

Journal ArticleDOI
TL;DR: A simple scheme for generation of high power in the midinfrared is demonstrated by using a 15 W thulium-doped fiber laser emitting at 1907 nm to pump a Q-switched Ho:YAG laser with excellent beam quality.
Abstract: A simple scheme for generation of high power in the midinfrared is demonstrated. By using a 15 W thulium-doped fiber laser emitting at 1907 nm to pump a Q-switched Ho:YAG laser, we obtained 9.8 W at 2096 nm at a 20 kHz pulse repetition rate with excellent beam quality. The output of this laser was used to pump a doubly resonant zinc germanium phosphide based optical parametric oscillator, and we obtained 5.1 W average power in the 3-5 μm range with M2≈1.8.

Journal ArticleDOI
TL;DR: This work investigates a novel procedure combining a pixel-rearrangement method and iterative backprojection (IBP) for reconstructing high-spatial-resolution images in an imaging system known as thin observation module by bound optics.
Abstract: A method for reconstructing high-spatial-resolution images in an imaging system known as thin observation module by bound optics (TOMBO) is reported. We investigate a novel procedure combining a pixel-rearrangement method and iterative backprojection (IBP). Pixel rearrangement has been used until now in TOMBO, and IBP is a digital superresolution technique. We verify the effectiveness of the combined procedure with simulated and experimental results.

Journal ArticleDOI
TL;DR: The principal component-based radiative transfer model (PCRTM) predicts the principal component (PC) scores of these quantities and has great potential for superfast one-dimensional physical retrieval and for numerical weather prediction large volume radiance data assimilation applications.
Abstract: Modern infrared satellite sensors such as the Atmospheric Infrared Sounder (AIRS), the Cross-Track Infrared Sounder (CrIS), the Tropospheric Emission Spectrometer (TES), the Geosynchronous Imaging Fourier Transform Spectrometer (GIFTS), and the Infrared Atmospheric Sounding Interferometer (IASI) are capable of providing high spatial and spectral resolution infrared spectra To fully exploit the vast amount of spectral information from these instruments, superfast radiative transfer models are needed We present a novel radiative transfer model based on principal component analysis Instead of predicting channel radiance or transmittance spectra directly, the principal component-based radiative transfer model (PCRTM) predicts the principal component (PC) scores of these quantities This prediction ability leads to significant savings in computational time The parameterization of the PCRTM model is derived from the properties of PC scores and instrument line-shape functions The PCRTM is accurate and flexible Because of its high speed and compressed spectral information format, it has great potential for superfast one-dimensional physical retrieval and for numerical weather prediction large volume radiance data assimilation applications The model has been successfully developed for the NAST-I and AIRS instruments The PCRTM model performs monochromatic radiative transfer calculations and is able to include multiple scattering calculations to account for clouds and aerosols

Journal ArticleDOI
TL;DR: The method of three-wavelength phase imaging is proposed to extend the axial range and reduce the effect of phase noise and the effectiveness of digital holography in high-resolution biological microscopy is demonstrated.
Abstract: We apply the techniques of digital holography to obtain microscopic three-dimensional images of biological cells. The optical system is capable of microscopic holography with diffraction-limited resolution by projecting a magnified image of a microscopic hologram plane onto a CCD plane. Two-wavelength phase-imaging digital holography is applied to produce unwrapped phase images of biological cells. The method of three-wavelength phase imaging is proposed to extend the axial range and reduce the effect of phase noise. These results demonstrate the effectiveness of digital holography in high-resolution biological microscopy.

Journal ArticleDOI
TL;DR: A new, high performance, hyperspectral microscope for biological and other applications that eliminates curvature and keystone artifacts that often plague spectral imaging systems is developed.
Abstract: We have developed a new, high performance, hyperspectral microscope for biological and other applications. For each voxel within a three-dimensional specimen, the microscope simultaneously records the emission spectrum from 500 nm to 800 nm, with better than 3 nm spectral resolution. The microscope features a fully confocal design to ensure high spatial resolution and high quality optical sectioning. Optical throughput and detection efficiency are maximized through the use of a custom prism spectrometer and a backside thinned electron multiplying charge coupled device (EMCCD) array. A custom readout mode and synchronization scheme enable 512-point spectra to be recorded at a rate of 8300 spectra per second. In addition, the EMCCD readout mode eliminates curvature and keystone artifacts that often plague spectral imaging systems. The architecture of the new microscope is described in detail, and hyperspectral images from several specimens are presented.

Journal ArticleDOI
TL;DR: Software with an interactive user interface that can be used to generate phase holograms for use with spatial light modulators to control the position, number, and type of optical traps.
Abstract: We have developed software with an interactive user interface that can be used to generate phase holograms for use with spatial light modulators. The program utilizes different hologram design techniques, allowing the user to select an appropriate algorithm. The program can be used to generate multiple beams and can be used for beam steering. We see a major application of the program to be in optical tweezers to control the position, number, and type of optical traps.

Journal ArticleDOI
TL;DR: High-speed complex full-range Fourier domain optical coherence tomography (FD-OCT) is demonstrated, which investigates a sweat duct in a finger pad in vivo and visualizes it with an acquisition time of 27 ms.
Abstract: High-speed complex full-range Fourier domain optical coherence tomography (FD-OCT) is demonstrated. In this FD-OCT, the phase modulation of a reference beam (M scan) and transversal scanning (B scan) are simultaneously performed. The Fourier transform method is applied along the direction of the B scan to reconstruct complex spectra, and the complex spectra comprise a full-range OCT image. Because of this simultaneous B-M-mode scan, the FD-OCT requires only a single A scan for each single transversal position to obtain a full-range FD-OCT image. A simple but slow version of the FD-OCT visualizes the cross section of a plastic plate. A modified fast version of this FD-OCT investigates a sweat duct in a finger pad in vivo and visualizes it with an acquisition time of 27 ms.

Journal ArticleDOI
TL;DR: The Monte Carlo-based inverse model of diffuse reflectance described in part I of this pair of companion papers was applied to a set of 17 malignant and 24 normal-benign ex vivo human breast tissue samples and produced a cross-validated sensitivity and specificity of 82% and 92%, respectively.
Abstract: The Monte Carlo-based inverse model of diffuse reflectance described in part I of this pair of companion papers was applied to the diffuse reflectance spectra of a set of 17 malignant and 24 normal-benign ex vivo human breast tissue samples. This model allows extraction of physically meaningful tissue parameters, which include the concentration of absorbers and the size and density of scatterers present in tissue. It was assumed that intrinsic absorption could be attributed to oxygenated and deoxygenated hemoglobin and beta-carotene, that scattering could be modeled by spheres of a uniform size distribution, and that the refractive indices of the spheres and the surrounding medium are known. The tissue diffuse reflectance spectra were evaluated over a wavelength range of 400-600 nm. The extracted parameters that showed the statistically most significant differences between malignant and nonmalignant breast tissues were hemoglobin saturation and the mean reduced scattering coefficient. Malignant tissues showed decreased hemoglobin saturation and an increased mean reduced scattering coefficient compared with nonmalignant tissues. A support vector machine classification algorithm was then used to classify a sample as malignant or nonmalignant based on these two extracted parameters and produced a cross-validated sensitivity and specificity of 82% and 92%, respectively.

Journal ArticleDOI
TL;DR: A through-focus comparison of azimuthal and radial polarization shows a measurable dependence of edge separation on polarization.
Abstract: Dark-field illumination provides an imaging mode that rejects specular light, thereby highlighting edge features. We analyze dark-field imaging by using cylindrical vector beam illumination with a confocal microscope equipped with a microstructure fiber mode filter. A numerical model based on rigorous coupled-wave analysis has been used to analyze the method. We acquired images of separated edges features to investigate the edge separation resolution of the method. A through-focus comparison of azimuthal and radial polarization shows a measurable dependence of edge separation on polarization.

Journal ArticleDOI
TL;DR: The real part of the complex refractive index of oxygenated native hemoglobin solutions dependent on concentration was determined in the wavelength range 250 to 1100 nm by Fresnel reflectance measurements.
Abstract: The real part of the complex refractive index of oxygenated native hemoglobin solutions dependent on concentration was determined in the wavelength range 250 to 1100 nm by Fresnel reflectance measurements. The hemoglobin solution was produced by physical hemolysis of human erythrocytes followed by ultracentrifugation and filtration. A model function is presented for calculating the refractive index of hemoglobin solutions depending on concentration in the wavelength range 250 to 1100 nm.