Showing papers in "Archives of Biochemistry and Biophysics in 1953"

TL;DR: The isolation of the two subunits of myosin, obtained after short digestion by trypsin, were termed L- and H-meromyosins (“L” stands for light, “H” for heavy).

TL;DR: The postulated mechanism of linoleate-oxidation initiation involves a direct reaction of linolesate peroxide with hematin catalysts and Propagation may take place in a manner similar to autocatalytic linoleates oxidation.

TL;DR: The hemoglobin mixture in hemoglobin C trait was found to have a significantly higher total solubility than normal adult hemoglobin, and Mixtures of normal adult and fetal hemoglobin also had higher solubilities than normaladult hemoglobin.

TL;DR: The biologically important antioxidant, α-tocopherol, was rapidly oxidized in the lipoxidase system and the effective concentrations were sufficiently low so that these two antioxidants might prove useful in protecting foods from this type of oxidation.

TL;DR: In this paper, it was shown that photooxidation of chymotrypsin with visible light in the presence of traces of methylene blue resulted in rapid inactivation of the enzyme.

TL;DR: It has been shown that densitygradient centrifugation followed by photometric scanning can be used as a method of analysis of potato yellowdwarf virus using sucrose solutions for the density gradient.

TL;DR: Several lines of evidence indicate that the IAA-oxidase system of peas consists of a light-activatable flavoprotein enzyme coupled through H 2 O 2 to a peroxid enzyme, which may be IAA itself and the substrate for the flavin enzyme is unknown.

TL;DR: The yeast survival curves are consistent with a theory that, in the haploid, cell division can be inhibited by inactivating any one of some tens of chromosomal sites by means of a single ionizing particle, whereas in the diploid it is necessary to inactivate both members of any one allelic pair of corresponding sites.

TL;DR: There is no “active” absorption of fructose and that glucose formation from fructose in the intestine is enzymically controlled, and substitution of sucrose by an equimolar concentration of invert sugar has no significant effect on the rates at which glucose and fructose appear on the serosal side of the intestine.

TL;DR: It is found that the calculation of turnover rate for any component of a metabolic network requires a complete knowledge of the structure of that network, as well as experimental study of the concentrations and specific activities of all its components.

TL;DR: 2,4-Dichlorophenol (DCP), frequently present as an impurity in samples of 2, 4-dichlor phenol, stimulates the activity of indoleacetic acid oxidase of pea epicotyls and is attributed to its cofactor-like activity.

TL;DR: The binding of sodium and potassium with 14 different proteins has been determined with collodion membrane electrodes and pepsin, β-lactoglobulin, zein, and three plasma protein fractions show a significant binding of both Sodium and potassium at pH 7.5.

TL;DR: It has been found that mitochondria of a higher plant, the mung bean, are capable of carrying on phosphorylation at the expense of substrate oxidation.

TL;DR: Procedures are described for determining glycogen in liver samples sufficiently small that a biopsy specimen may also provide material for other analyses and evidence is presented to show that glycogen does not vary greatly from site to site within one lobe of liver.

TL;DR: Using labeled substrates in vitro the oxidation of glycine α- and β-carbons was observed in rat liver and kidney but not in other organs, and evidence has been presented to indicate this occurs via a peroxidative activity of catalase.

TL;DR: In this paper, the green alga Chlorella pyrenoidosa has been demonstrated for demonstrating requirements for the inorganic micronutrients calcium (or strontium), copper, and molybdenum, in addition to previously established requirements for iron, manganese and zinc.

TL;DR: Tissue concentration patterns were remarkably constant between species and between tissues, and Cesium-137 was accumulated in tissues against a concentration gradient, resembling potassium in this respect.

TL;DR: Binding of calcium ions by bovine serum albumin has been found to be essentially independent of temperature over the range 0 to 25 °C, while pH has a very pronounced effect.

TL;DR: Evidence is presented indicating that the reaction product is galactosamine 1-phosphate, which was found to be more resistant to acid hydrolysis than aldose 1- phosphates.

TL;DR: It was concluded that the activation involves the binding of one single metal ion per molecule of enzyme and the ionic radius of a given metal ion is the main property determining its activating power.

TL;DR: A method of fibrinogen analysis by turbidity with (NH4)2SO4 is described, and its potential diagnostic value is indicated.

TL;DR: Renin has been purified 56,000-fold in the ten fractionation steps of this procedure, and it has a specific activity about 40 times greater than that of renin preparations previously described.

TL;DR: It is suggested that its mechanism of cellulose breakdown is unienzymatic, and evidence supporting this mechanism is discussed.

TL;DR: The most cellular marrow samples contained the least water and the most lipide, which is a reversal of the order found in normal animals.

TL;DR: While copper supplementation influenced the liver enzymes, it had no effect in correcting the growth inhibition produced by zinc.

TL;DR: Extracts of Rhodospirillum rubrum prepared by sonic disintegration have been shown to contain the following enzymatic activities: cytochrome oxidase; DPNH- and TPNH -cytochrome c reductase; “malic” enzyme; and the dehydrogenases for succinic, malic, and lactic acids.

TL;DR: The structure of the principal oligosaccharide formed in the honey invertase-sucrose reaction is consistent with the view that glucose transfer is an integral part of this reaction and is further evidence of the glucoinvertase nature of honey invertedase.