Showing papers in "Archives of Biochemistry and Biophysics in 1959"

TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.

TL;DR: The results suggest that the mechanism involved is non-enzymic and consists of a cooxidation of ascorbic acid and unsaturated fat mediated by a metal ion.

TL;DR: Using UDPG labeled in the glucose moiety, it was found that the radioactivity was transferred to the glycogen from which it could be removed as maltose with β-amylase or as G-1-P with phosphorylase, suggesting that the glucose residue becomes linked α (1 → 4) to the polysaccharide.

TL;DR: Isolated mitochondria suspended in 0.15 M NaCl at 37 °C deteriorate by a hematin-catalyzed lipide peroxidation, characterized by oxygen absorption, thiobarbituric acid reaction, and enzyme inactivation.

TL;DR: A Ca++-activated enzyme system derived from the soluble fraction of guinea pig liver which incorporates a wide variety of amines into proteins is described and possible mechanisms for the reaction and the relation of this system to other amine-incorporating reactions are discussed.

TL;DR: Adding tocopherol was effective both in inhibiting lipide peroxidation and stabilizing DPNH-cytochrome c reductase in isolated mitochondria in Vitamin E-deficient rabbits.

TL;DR: The data obtained provide a relatively complete picture of the phase relations in aqueous solutions of mono- and disodium and potassium phosphates in the freezing zone and the effect of simultaneous precipitation of two salts on pH depended on the type of salts precipitating as well as the initial pH.

TL;DR: 5-Hydroxytryptamine, tryptamine, tyramine, 3,4-dihydroxyphenylethylamine and norepinephrine have been identified and assayed in a large number of fruits and vegetables and the significance of these findings with respect to plant physiology and to dietary effects in animals is discussed.

TL;DR: Detailed methods for the isolation, detection, and measure of tissue nucleotides by procedures appropriate to radiochemical investigation have been presented and provide sensitivity appropriate to small tissue samples and products of high radiochemical purity.

TL;DR: The high value of the turnover of an enzyme acting in a heterogeneous medium is readily explained by interactions of fundamental importance between lipase and insoluble esters.

TL;DR: Plasma amino acid changes of the adult dog after a meal have been found to be directly dependent upon the amino acid composition of the protein ingested if it is postulated that the individual essential amino acids are removed from the blood by the body tissues at rates proportional to the amino acids requirements of the dog.

TL;DR: The relationships show, among other things, that measurements of isotopic exchange rates at equilibrium can lead to determinations of dissociation constants for enzyme-substrate intermediates in some simple systems and in detection of compulsory orders of combination of reactants with an enzyme.

TL;DR: The excellent symmetry of the patterns obtained in this medium at relatively high gluten concentration recommends its use for following the progress of fractionation experiments and for investigating the effect on gluten composition of such variables as gluten quality and method of preparation.

TL;DR: Ultraviolet absorption spectra were determined for highly purified samples of diphosphopyridine nucleotide, finding increased absorbancies at the absorption maxima characteristic of both the purine and pyridine moieties interpreted as evidence for intramolecular interactions between the two moieties in the intact dinucleotides.

TL;DR: Rats bearing the rapidly growing Walker tumor were subjected to the deficient diets, and all gained in weight by an amount nearly equivalent to the weight of the tumor, the carcasses losing weight to about the same extent as their normal controls on the same diets.

TL;DR: In this article, a relatively complete picture of phase relations during precipitation of ice and one or more salts was obtained, and several eutectic points of solutions containing chlorides in addition to sodium and potassium phosphates were determined.

TL;DR: A simplified heat test for the detection of Bence-Jones protein in urine is proposed based on a set of conditions for heating which resulted in the heavy precipitation of a wide variety of BENCE-Jones proteins but not of normal serum proteins.

TL;DR: DPN was determined under corresponding conditions, using as indicator the competition of DPNH and DPN for the same binding site(s) as well as the effects of formate and chloride, interfering with the kinetics particularly in acid solution.

TL;DR: The use of hydroxylamine as a replacing amine permits the measurement of the reactivity of acid-soluble peptides and proteins readily by determining the extent of hydroXYlamine incorporation colorimetrically.

TL;DR: It has been shown that the increase in the activities of a phosphatase and an amylase in the cotyledons of germinating peas is the result of a process which is energy dependent and inhibited by chloramphenicol and p -fluoro-phenylalanine.

TL;DR: Increased persistence of digitonin-precipitable “higher-counting companions” of cholesterol indicate that a site of inhibition is between these substances and cholesterol.

TL;DR: The introduction of a stoichiometric excess of ethereal LiAlH 4 into hydrocarbon solutions of tocopherol esters was found to result in a quantitative cleavage to the free alcohols.

TL;DR: A comparative biochemical study was made on the vitreous body of the following animals: squid, frogs, tortoises, chickens, turkeys, pigeons, owls, rabbits, guinea pigs, sheep, cattle, and owl monkeys, finding that hexosamine-containing compounds other than hyaluronic acid, soluble proteins, and collagen were unevenly distributed.

TL;DR: Carp liver mitochondria were shown to contain an enzyme system that will oxidize fatty acids in the presence of adenosine triphosphate, cytochrome c, and Mg++, and the addition of a sparking substance considerably enhanced the rate of oxidation.

TL;DR: Despite a wide divergence in linoleic acid levels, the total unsaturated acids remained constant and first-order rate constants for the slower rate are higher for females and immature males than for mature males.