Showing papers in "Archives of Biochemistry and Biophysics in 1961"

TL;DR: Method has been applied to a study of hydroxyproline distribution in cell particulates, tissue fluids, and purified plant and animal proteins, and significant amounts of hydroXYproline were found in crystalline preparations of pepsin, elastase.

TL;DR: Preliminary studies indicate that benzoyl dl -arginine p -nitroanilide hydrochloride is also hydrolyzed by papain, and that of l -LPA is in a more alkaline region than normally found for trypsin substrates.

TL;DR: A rapid and sensitive method has been described for the measurement of amino acid incorporation in cell-free systems and has been applied to both C14- and H3-labeled proteins.

TL;DR: The quantitative distribution of sodium-potassium-activated adenosine triphosphatase, which had been shown to play a role in active transport of sodium and potassium in erythrocytes, has been determined in 36 tissues of the cat and in human retina, ciliary body, and ery Throcytes.

TL;DR: Light-dependent production of molecular hydrogen in the presence of utilizable citric acid cycle intermediates has been demonstrated in resting cell suspensions of Rhodospirillum rubrum, and evidence is presented in support of the conclusion that ammonia, or a derivative metabolite, acts as a repressor of synthesis of one or more required protein components.

TL;DR: The experiments provide the first demonstration of the spontaneous synthesis of adenine from simple compounds of carbon and nitrogen under conditions presumed to have existed on the primitive earth.

TL;DR: Starch gel electrophoresis using aluminum lactate buffer containing 3 M urea demonstrated nine components in wheat gluten; four were not previously detected by the Tiselius method, and one of the components appears to be high molecular weight and unable to migrate in starch gels.

TL;DR: A rapid method for diamine oxidase assay is described, applicable using radioactive putrescine as substrate, but does not work with radioactive histamine.

TL;DR: Purification steps, additional to those reported in the literature, using diethylaminoethylcellulose and Amberlite CG-50 chromatographic columns, have been worked out for “apyrase.”

TL;DR: The distribution of carbamyl phosphate synthetase (CPS) and/or ornithine transcarbamylase (OTC) was determined in 17 different rat tissues and it was found that both CPS and OTC first appeared late in fetal life in the rat and did not reach adult levels until after birth.

TL;DR: A new mode of hormonal control of glycogen metabolism is demonstrated by increasing enzyme activity after insulin treatment, which supports the role of UDP-glucose glycogen transglucosylase in glycogen 1,4-bond synthesis.

TL;DR: Solutions of DNA in a variety of nonaqueous solvents can be prepared by dialysis in the absence of electrolyte, and macromolecular and optical criteria indicate that, in these solutions, DNA is denatured.

TL;DR: Observations indicate the possibility that respiratory control is associated only with the first phosphorylation site in the DPNH oxidase system of beef heart mitochondria.

TL;DR: The remarkable metabolic activity and high concentrations of phosphatidyl glycerol and the galactolipids in Chlorella attest to their importance in photosynthesis.

TL;DR: By a mild treatment using an ion-exchange resin, the excess nucleotide present in G-actin solutions can be removed without appreciable direct loss of the polymerizability of actin.

TL;DR: It is proposed that the 705-mμ absorption band is due to a complex of cytochrome and chlorophyll a and that this complex acts as the “reaction center” in the photosynthetic apparatus.

TL;DR: A procedure is described for the isolation of empty tubular cell membrane-like structures from rat skeletal muscle which resulted in a purification of about 140-fold in terms of protein content per tube.

TL;DR: A mechanism in which water molecules act to stabilize the collagen-fold configuration through formation of doubly hydrogen-bonded bridges between adjacent carbonyl groups is proposed in terms of an intramolecular crystallization process originating from preformed nuclei.

TL;DR: An investigation of heparin fractionated by cetylpyridinium chloride precipitation has shown that variations in anticoagulant activity are related to molecular weight.

TL;DR: In this article, the influence of substituents on the orientation of aromatic hydroxylation by the dihydroxyfumarate-peroxidase-oxygen and the ascorbate-ironoxygen systems, has been studied.

TL;DR: The synthesis of phospholipid in the liver and uteri was not inhibited, but a stimulatory response to puromycin was observed, and the role of protein synthesis in certain physiological processes was studied.

TL;DR: Spectral properties of the enzyme, relation of activity to pH, and the effect of heat denaturation are described, which found the enzyme to be homogeneous, migrating in the form of a sharply defined band.

TL;DR: A method for the separation of neuraminidase from the Asian and PR8 strains of influenza virus has been described and some preliminary studies on the kinetic properties of the enzyme have been presented.

TL;DR: The conversion of indole to 3-hydroxyindole has been demonstrated, raising the possibiltiy that the indican of the urine might be derived from hydroxylation of indoles in the liver rather than from hydoxylation by bacteria in the gut.

TL;DR: Porcine trypsin is different from its bovine and ovine counterparts, and the differences are striking in electrophoretic behavior and stability in alkaline media.

TL;DR: The fatty acid contents of mitochondria from chicken liver, beef heart, rat liver, catfish liver, carp liver, salmon liver, and salmon heart were determined by gas-liquid chromatography of the methyl esters, and fish mitochondria were generally low in essential fatty acids, but contained high levels of docosapentaenoic and docosahexaenoic acids.

TL;DR: It is tentatively concluded that the glyoxalate cycle is inoperative in this organism and the accumulation of organic acids in the insect is discussed.

TL;DR: It was concluded that the formation of the iron complex might not only protect the active center so that the chromogenic capacity was retained but also stabilized the molecule in some manner so that modification of other parts of the molecule could occur without denaturation.

TL;DR: Plastocyanin, a copper protein originally discovered in Chlorella ellipsoidea, was found to occur in green leaves of the following plants: spinach, parsley, carrot, turnip, Japanese scallion, and crown daisy and it was inferred that plastOCyanin is associated with the chlorophyll-bearing subparticulate components of chloroplasts.