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Showing papers in "Archives of Biochemistry and Biophysics in 1969"


Journal ArticleDOI
TL;DR: A high resolution gel electrophoresis of histone is described, capable of distinguishing between histone fractions whose mobilities differ by as little as 1% under the conditions of pH and urea concentration employed.

2,292 citations


Journal ArticleDOI
TL;DR: A technique is described for the precipitation of the bulk of the plasma proteins with caprylic (octanoic) acid without affecting IgG, ceruloplasmin, and part of the IgA.

838 citations


Journal ArticleDOI
TL;DR: Aryl hydrocarbon hydroxylase from rat liver metabolizes a variety of polycyclic hydrocarbons and is inducible in fetal cell cultures derived from whole hamster, mouse, rat, and chick.

422 citations


Journal ArticleDOI
TL;DR: The data suggest that the A1 protein is very likely the only encephalitogenic basic protein of native myelin, constituting approximately 30% of the total myelin protein.

409 citations


Journal ArticleDOI
TL;DR: The poor cleavage of a -Met-Thr- bond in catalase by cyanogen bromide has been improved by modifying the conditions of the reaction by increasing the concentration of the reactants.

270 citations


Journal ArticleDOI
TL;DR: Tricarballylate is a substrate for citrate cleavage enzyme and can be demonstrated both by the disappearance of CoA and, in the presence of hydroxylamine, by the appearance of a hydroxamate, which indicates that tricarballylyl-CoA is formed in the reaction.

249 citations


Journal ArticleDOI
TL;DR: In this paper, α-amylase from Aspergillus oryzae was purified by gel filtration and ion-exchange chromatography, and showed that the mixture contained glucosamine, mannose, galactose, arabinose, and xylose.

232 citations


Journal ArticleDOI
TL;DR: The highly extended conformation of the Al molecule, found from viscosity studies, is compatible with its resistance to denaturation; no loss in encephalitogenic activity was found following heating at 100 ° for 1 hr, treatment with 8 m urea for 8 hr, or incubation at pH 10.25.

164 citations


Journal ArticleDOI
TL;DR: Observations indicate that caution is required in the evalution of ANS fluorescence data in terms of conformational changes in membrane proteins alone, since the contribution of phospholipids to the fluorescence is significant.

158 citations


Journal ArticleDOI
TL;DR: Cholic acid, when given with the diet, also acts at this enzymic step as inhibitor of cholesterol biosynthesis and is shown to be much more effective than dietary cholesterol, but the concentration levels of cholic acid in liver do not show alterations which can be correlated with the observed rhythmic activity changes under conditions of normal feeding.

157 citations


Journal ArticleDOI
TL;DR: The findings suggest that the pathways for the biosynthesis of the gibberellin hormones in higher plants and the steroid hormones in animals are analogous in that both involve an initial sequence of nonoxidative steps involving water-soluble substrates and soluble cytoplasmic enzymes followed by a secondary sequence of aerobic, oxidative steps involving hydrophobic substrate and membrane-bound enzymes.

Journal ArticleDOI
TL;DR: The results obtained indicate that the cysteine-CuII complex represents the real intermediate catalyst in the copper-catalyzed oxidation of cysteines, and its anaerobic reduction are very complex reactions.

Journal ArticleDOI
TL;DR: There is strong evidence that intramitochondrial malic enzyme activity is the major source of NADPH for 11β-hydroxylation in bovine adrenal cortex when either malate or succinate are the source of reducing equivalents.

Journal ArticleDOI
TL;DR: It seems most likely that the major urinary detoxification metabolite of selenite is trimethyl selenide, which was isolated from rat urine by ionexchange chromatography and precipitation as the reineckate salt.

Journal ArticleDOI
TL;DR: In this paper, an electron microscope study of rat liver mitochondria revealed the following ultra-structural differences due to EFA deficiency: inner membranes were more irregularly organized in vitro, and the mitochondria were larger in situ, and enlarged more upon isolation than did mitochondria from rats on EFA-supplemented diets.

Journal ArticleDOI
TL;DR: Comparison of the binding energies of several heterocyclic compounds structurally related to benzothiazole suggests that the nitrogen atom of the benzothsiazole ring may be important in orienting the compound to a fixed position in the luciferinbinding site.

Journal ArticleDOI
TL;DR: The similarities in composition at both the 1- and 2-positions between triglycerides and diacyl PC and between GEDE and alkyl acyl PC suggest a loss of acyl CoA: lysophosphatide acyl trausferase enzymes which are present in normal tissue.

Journal ArticleDOI
TL;DR: The data indicate that selectivity of diglyceride species characterizing each phosphoglyceride class occurs in rat liver, and none of the carbon number distributions of the diglycerides derived from each of the phosphoglycers resembled the 1,2-diglycerides obtained from rat liver triglycerides.

Journal ArticleDOI
TL;DR: The total Ca ++ -uptake of fragmented sarcoplasmic reticulum (FSR) of either white or red skeletal muscle was optimal between pH 6.5–7.0 and 5.7–6.4 in the presence or absence of oxalate, respectively.

Journal ArticleDOI
TL;DR: The purified adenyl cyclase fraction did not contain cyclic 3′,5′-nucleotide phosphodiesterase activity, exhibited optimal activity at pH 8.0, and was inhibited by Zn 2+ or Ca 2+ .


Journal ArticleDOI
TL;DR: Dimensional characteristics of the nature of the hapten combining site for rabbit anti-DNP antibodies with various spin-labeled haptens have been determined using the method of Electron Spin Resonance andspin-labeling.

Journal ArticleDOI
TL;DR: Two mucins were readily soluble in water, gave extremely viscous solutions, and had similar compositions that closely resembled that of a single mucin fraction isolated earlier in these laboratories, however their compositions differ markedly from those of two porcine submaxillary glycoproteins described by Katzman and Eylar (3).

Journal ArticleDOI
TL;DR: Two enzyme systems, found in 105,000g particulate fraction of epiphyseal cartilage homogenates, appear to be the critical sites of manganese function.


Journal ArticleDOI
TL;DR: Thyroxine-binding globulin has been separated from human serum by affinity chromatography using Sepharose to which l -thyroxine had been covalently attached and produced a single stained band on analytic disc-gel electrophoresis.

Journal ArticleDOI
TL;DR: Increased activity of this enzyme is an early event involved in the phagocytosis-associated increase in this metabolic pathway, it can be postulate.

Journal ArticleDOI
TL;DR: The properties of the methemerythrin complexes are consistent with the presence of a dimeric pair of high spin iron (III) atoms antiferromagnetically coupled via an oxo bridge and the proposed structure predicts that considerable conformational change occurs on oxygenation.

Journal ArticleDOI
TL;DR: The interaction of human deoxy-, oxy-, and methemoglobin with 2,3-diphospho-glycerate, adenosine tri-, di-, and monophosphates, ribose-5-ph phosphate, pyrophosphate, tripolyphosphate and tetra-, and hexametaphosphate was studied by equilibrium dialysis.

Journal ArticleDOI
TL;DR: The rate-limiting process in vitamin D deficiency appears to be the initial transfer of calcium across the brush border surface into the columnar epithelial cells.