Showing papers in "Archives of Biochemistry and Biophysics in 1972"

TL;DR: The rate of collagen synthesis relative to the rate of synthesis of noncollagen proteins expressed as radioactivity incorporated per milligram of cellular protein was determined in several lines of cultured fibroblasts using an assay which measures [14C]proline incorporation into the polypeptide chains of collagen.

TL;DR: This study directly compares the specificities of the structurally similar hydroxylating enzymes, aldehyde oxidase and xanthine oxidase, and finds some specificity differences with respect to the chemical nature of substituents.

TL;DR: This method requires very small amounts of protein and allows determination of low as well as very high dissociation constants with equal reliability.

TL;DR: Evidence is presented that the bovine kidney and heart dihydrolipoyl transacetylases are very similar and that each enzyme does indeed consist of 60 apparently identical polypeptide chains.

TL;DR: A flavoprotein-oxidase isolated from pig hepatic microsomes catalyzes the NADPH- and oxygen-dependent N -oxidation of a variety of lipid-soluble sec - and tert -amines, with one or two exceptions the isolated oxidase does not catalyze the oxidation of primary amines.

TL;DR: Preliminary data indicate that the molar concentration of the kinase and the phosphatase is about an order of magnitude less than that of their protein substrate, pyruvate dehydrogenase.

TL;DR: A comparison of the half-lives of histone fractions and their methyl lysine and methyl arginine components indicated that histone methylation was irreversible.

TL;DR: Dihydrolipoyl transacetylase markedly stimulates the rate of phosphorylation of PDH by PDH kinase and is found to lower the apparent Km of the kinase for PDH from about 20 μ m to about 0.6 μ m.

TL;DR: The activity which had been decreased by starvation was rapidly induced to a very high level on refeeding of the animals and returned gradually to the steady-state level on continuation of feeding, suggesting that the dietary regulation involved the change of mainly the terminal component of the desaturation system, cyanide-sensitive factor.

TL;DR: Nembutal was shown to influence substrate concentrations severely, independent on narcosis-induced anoxia, and AMP was found to be a highly sensitive parameter to stress.

TL;DR: Pretreatment of female rats with pregnenolone-16α-carbonitrile (PCN) for 212 days resulted in a significant increase in cytochrome P-450 content and NADPH-cytochrome c reductase activity in hepatic microsomes, and studies on the induction of enzymes that metabolize the above three substrates revealed that PB, PCN, and 3-MC exerted their greatest stimulatory effect on benzphetamine N-demethylation,

TL;DR: It has been shown that the catalase immediately after isolation has a heterogeneity different than they describe and that alteration with time does occur and the cause of the heterogeneity has not been determined.

TL;DR: This observation supports the thesis that cyclic AMP mediates the steroidogenic action of ACTH on the adrenal cortex and the bearing of these results on the second messenger theory is discussed.

TL;DR: NADH-cytochrome c reductase from plant and animal microbodies and microsomes was insensitive to antimycin A, which inhibited the activity in the mitochondrial fractions, and exhibited a broad pH optimum between pH 6 and 9.0.

TL;DR: It appears that the Cd-binding protein is an inducible cellular component which serves to sequester toxic cadmium ions.

TL;DR: Using polyacrylamide gel disc electrophoresis, a simple and sensitive stain method for glucan phosphorylase (EC 2.4.1.1) was developed and 0.3–1.5 μg or 1–5 units of phosphoryLase could be demonstrated as a sharp band within a few hours.

TL;DR: The usual indicator of essential fatty acid deficiency, 20:3ω9, accumulated in all of the tissues examined except the retina and the photoreceptor membranes, which must have a mechanism for conserving its abundant supply of ω3 polyunsaturates.

TL;DR: The blue lupin β-cyanoalanine hydrolase was found to be highly specific for β-cycloalanine, distinct from other plant, bacterial and fungal nitrilases, asparaginases or glutaminases and plant hydroxynitrile lyases.

TL;DR: Rat brain hexokinase has been purified to homogeneity as judged by disc-gel electrophoresis, isoelectric focusing, and analytical ultracentrifugation, suggesting extensive sequence homology.

TL;DR: A free interface diffusion technique for the crystallization of proteins for X-ray crystallography is described, which allows experiments to be carried out routinely with small quantities of protein.

TL;DR: The new spectral species is interpreted to be an oxygenated form of cytochrome P-450, presumably a ternary complex of oxygen, camphor, and ferrous cyto Chrome, and undergoes slow decomposition to the ferric form of the hemoprotein and is most stable in the pH range 7–8 and at temperatures below 10 °.

TL;DR: The in vivo rate of lipogenesis was markedly decreased for 150 min following the administration of (−)-hydroxycitrate either iv (0.017 mmoles/kg) or ip ( 0.0263 mmoles /kg) and fatty acid and cholesterol synthesis were significantly inhibited by the oral administration.

TL;DR: Guinea pig heterophil and eosinophil peroxidases have substantially different properties despite their location in analogous organelles of closely related cells, indicating that aggregation occurred during purification.

TL;DR: The formation of a complex between calcium and NADH in the nonpolar region of the mitochondrial membrane is proposed and the physiological implications of this complex are discussed.

TL;DR: The results suggest that the changes in catalytic properties are associated with the removal of a tryptophan-containing peptide or peptides with molecular weights totaling approximately 6000 per subunit, and that the alkaline enzyme previously studied in this and other laboratories is a modified form of the native neutral fructose diphosphatase.

TL;DR: A basic protein exists in rabbit sciatic nerve myelin that appears to have the same amino acid sequence as the rabbit A1 protein, a basic protein of central nervous system myelin, and the amino acid sequences proposed for the two rabbit proteins are compared with the bovine and human A1 proteins.

TL;DR: Acid hydrolysis, digestion with trypsin and chymotrypsin, and group determination experiments demonstrated that alytesin and bombesin are tetradecapeptides differing only in the second and sixth residues from the NH2-terminus.

TL;DR: The effects of methionine and cyst(e)ine on the betaine-chomocysteine methyltransferase may be significant in the regulation of methamphetamineionine metabolism in the intact animal.