Showing papers in "Archives of Biochemistry and Biophysics in 1976"

TL;DR: With this method, rachitic chick plasma, plasma from anephric patients, and plasma from patients suffering severe endstage renal failure show no detectable 1,25-dihydroxyvitamin D, while normal human values have been found to be 29 ± 2 pg/ml.

TL;DR: Data obtained are in accord with a peroxidase-like mechanism for the action of cytochrome P-450, which catalyzes the hydroperoxide-dependent hydroxylation of a variety of substrates in the absence of NADPH, NADPHcytochromeP-450 reductase, and molecular oxygen.

TL;DR: Rat liver xanthine dehydrogenase, type D, has been isolated directly from crude extracts as an antibody complex and its properties compared with those of two oxidase forms of the enzyme, heat- treated type O and trypsin-treated type O, also isolated as antibody complexes.

TL;DR: A marked increase in the levels of NAD is observed in 3T3 cells, but not in SVT2 cells, at cell densities just prior to where 3T 3 cells enter density-dependent inhibition of growth, correlated with the cessation of DNA synthesis.

TL;DR: Observations indicate that VO3− can bind quite specifically at the phosphate-binding site on the enzyme, which suggests that the enzyme-complexed inhibitor may have some resemblance to the metastable intermediate formed during the hydrolysis of phosphate esters.

TL;DR: Mucous glycoproteins isolated by agarose gel filtration from nonpurulent tracheobronchial secretions and purulent sputum which had been reduced, carboxymethylated and, in the case of purulent secretions, treated with deoxyribonuclease displayed only minor compositional differences from their counterparts in nonpurulence secretions.

TL;DR: The xanthine-oxidizing enzyme of rat liver has been purified as an NAD+-dependent dehydrogenase ( type D) and as the O2-dependent oxidase (type O).

TL;DR: It is proposed that bestatin and its active isomers are effective due to a mechanism other than a chelating action at the active center because of a metallo-exopeptidase mechanism.

TL;DR: It is demonstrated that, in human liver, these two β-glucosidases differ in terms of their subcellular localization, chromatographic behavior on ion-exchange columns, substrate specificity, and sensitivity to inhibition or activation by sodium taurocholate and phospholipids.

TL;DR: The catalytic properties of transfer enzyme AA are very similar to those of transferase B although the two proteins differ in their ability to bind bilirubin and other ligands, in their amino acid composition, and in their immunological properties.

TL;DR: The α- d -GNAc-(1 → 4)- d -Gal and the two trisaccharides are the structures involved in the specificity of the new antigenic determinant of hog gastric mucin blood group A + H substance unrelated to ABH activity as shown by their ability to inhibit precipitation of hog blood group substance by concanavalin A and by a human antiserum to the new determinant.

TL;DR: The findings suggest that stimulation of this enzyme activity in the liver is closely related to the rate of degradation of cytochrome P-450 heme.

TL;DR: The secondary structure of the peptide segment around the carbohydrate-peptide linkage in glycoproteins was predicted by using the Chou and Fasman determination and it seems that carbohydrate moiety takes place outside the glycoprotein.

TL;DR: Although the 3.7S protein from chick mucosa has properties consistent with its possible role as a receptor, the 5-6S macromolecule does not appear to have ''receptor''-like properties.

TL;DR: The results indicate that the antigenic determinants of phospholipase depend on the charge, the amino acid sequence and the conformation of the molecule.

TL;DR: Incomplete development of chloroplast lamellae occurred when etiolated pea plants were greened under cycles of 2 min light, 118 min dark, and a biphasic decrease in quantum requirement for whole chain electron transport correlated directly with the appearance of grana during the final steps of membrane assembly.

TL;DR: Two NAD-dependent aldehyde dehydrogenase enzymes from rat liver mitochondria have been partially purified and characterized and by utilization of the different substrate properties of these two enzymes it was possible to demonstrate a time-dependent release from digitonin-treated Liver mitochondria.

TL;DR: Catabolism of several amino acids from the lumen plus glutamine from arterial blood may provide an important energy source in small intestine.

TL;DR: The results are interpreted as indicating a role for O2− plus H2O2 in the oxygen enhancement of radiation lethality, and a scheme is proposed which is consistent with the observations.

TL;DR: Using this method, it was found that the four oligosaccharides which contain O -α- l -fucosyl-(1 →∗2)- O -β- d -galactosyl groupings occur in milk from donors with blood type Le(b+) but not in Milk from Donors with blood types Le(a+).

TL;DR: Activation of the phosphodiesterase by lysophosphatidyl choline was characterized by a high degree of positive cooperativity, exhibiting a Hill coefficient of 3.8.

TL;DR: Dihydroorotate dehydrogenase from rat liver is found to be located on the outer surface of the inner membrane of mitochondria, and no active transport of either dihydroOrotate or orotate is required in pyrimidine biosynthesis.

TL;DR: A second lectin with a high activity for nonreducing 2-acetamido-2-deoxy-α- d -glucopyranosyl residues has been isolated from the seed extracts of Bandeiraea simplicifolia by affinity chromatography on chitin.

TL;DR: It is concluded that the cytochrome P -450-linked monooxygenase system present in the intestinal mucosa differs markedly from the hepatic system with regard to induction properties, substrate specificity, and pattern of BP metabolites.

TL;DR: The results suggest that the trypsin-susceptible bond in the native Type III collagen molecule resides in a region characterized by a relative lack of the normal collagen helicity.

TL;DR: Ouabain-binding vesicles were distinct from the major vesicle population, but were mechanically linked until the linkage was destroyed by mechanical disruption, and may be identified specifically after homogenization.

TL;DR: The weak luminescence that accompanies the aerobic xanthine oxidase reaction is inhibited by superoxide dismutase, by catalase, and by scavengers of hydroxyl radicals, and it is also entirely dependent upon the presence of carbonate.

TL;DR: It is concluded that in heart cells energy can be transported by creatine phosphate molecules only and the kinetic theory based on this assumption allows a quantitative explanation of the observed dependences.

TL;DR: The results suggested, therefore, that elastin may be secreted by the same system of vacuoles that is involved in the secretion of procollagen.

TL;DR: The mast cell chymotrypsin-like enzyme purified in this way migrated on dodecyl sulfate-gel electrophoresis as a single major band with an estimated molecular weight of 29,000.