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Showing papers in "Archives of Biochemistry and Biophysics in 1977"


Journal ArticleDOI
TL;DR: The analysis of solubility and Chromatographic data shows that the overall salt effect can be described by the two antagonistic effects of salts on electrostatic and hydrophobic interactions.

850 citations


Journal ArticleDOI
TL;DR: Evidence for the role of ubiquinone as peroxide generator is provided by the generation of O 2 − and H 2 O 2 during autoxidation of quinols.

837 citations


Journal ArticleDOI
TL;DR: A method for the isolation of homogeneous Escherichia coli RNA polymerase holoenzyme is described, in which only small amounts of σ subunit are lost and RNA polymerases holoenzymes are well separated from the core polymerase.

269 citations


Journal ArticleDOI
TL;DR: A sensitive and convenient augmentation assay for superoxide dismutase has been devised and appears to be remarkably free of interferences and can be applied to crude soluble extracts of biological samples.

243 citations


Journal ArticleDOI
TL;DR: It is demonstrated that the expected secondary amine linkages are formed with the ϵ-amino groups of lysine in synthetic glycoproteins prepared and compared with bovine serum albumin conjugates by amino acid analysis after acid hydrolysis.

219 citations


Journal ArticleDOI
TL;DR: Hematocytes isolated from fed or starved rats were rapidly lysed using the recently described technique of turbulent flow to evaluate the mass action ratios of both cytosolic and mitochondrial enzymes.

194 citations


Journal ArticleDOI
TL;DR: When benzylamphetamine alone is added in control experiments to correct for the NADPH and O 2 consumption not associated with benzphetamine hydroxylation, the expected 1:1:1 stoichiometry for NADPH oxidation, O 2 Consumption, and formaldehyde formation in the hydroxyation reaction is observed.

192 citations


Journal ArticleDOI
TL;DR: The data indicate that metabolism of glutamine to glutamate occurs in mitochondria of both villus and crypt cells and that phosphate-dependent glutaminase is the major glutamine-utilizing enzyme of small intestine.

172 citations


Journal ArticleDOI
TL;DR: The substrate specificity of the enzyme was in agreement with its proposed function in flavonoid biosynthesis, and the two cosubstrates, ATP and CoA, exhibited sigmoidal saturation kinetics, which were interpreted as indicating homotropic, allo-steric effects.

154 citations


Journal ArticleDOI
TL;DR: Optimal restoration of the (Ca 2+ + Mg 2+ )-ATPase activity in the partially lipid-depleted membranes was obtained with oleate, and only phosphatidyl serine reactivated significantly.

147 citations


Journal ArticleDOI
TL;DR: The behavior of mitochondrial respiratory control is shown to be quantitatively consistent with a proposed mechanism in which the regulation occurs in the reaction of oxygen with cytochrome oxidase.

Journal ArticleDOI
TL;DR: Data obtained by the disruption technique are compared with other previously published data obtained using either digitonin treatment of isolated hepatocytes or nonaqueous solvent extraction of lyophilized freeze-clamped perfused livers.

Journal ArticleDOI
TL;DR: Results indicate that R. capsulata requires a terminal electron acceptor for anaerobic dark growth and that DMSO can serve that function.

Journal ArticleDOI
TL;DR: The evidence suggests that the plasma membrane enzymes, i.e., NADPH oxidase and adenylate cyclase, are controlled, in part, by the intracellular redox potential.

Journal ArticleDOI
TL;DR: In cultured hamster cells a single synthetase can successively add at least three glutamates to H 4 PteGlu, which is responsible for their triple auxotrophy.

Journal ArticleDOI
TL;DR: Part of the formaldehyde formed from the sec-N-methylamine arises from non-cytochrome P-450-dependent oxidation catalyzed by pig, hamster, and rat liver microsomes, demonstrating that formaldehyde can be inhibited by adding methimazole, a non-formaldehyde-producing substrate specific for the microsomal mixed-function amine oxidase, to the reaction media.

Journal ArticleDOI
TL;DR: The stimulation of the 25-hydroxyvitamin D 3 -1-hydroxylase by parathyroid hormone was of a smaller magnitude than that of the estradiol, and the effects of the two hormones were additive, suggesting that they function by a different mechanism.

Journal ArticleDOI
TL;DR: Free radical adducts of phenyltertiarybutylnitrone (PBN) were produced as detected by electron spin resonance during induced lipid peroxidation of microsomes with a system consisting of NADPH, Fe 2+ , and pyrophosphate, suggesting an alternate mechanism for the activity of these compounds as ultimate carcinogens.

Journal ArticleDOI
TL;DR: Although no evidence for membrane sulfhydryl oxidation or lipid peroxidation can be demonstrated in red cells exposed to DHF, the membranes of these cells were found to retain a green pigment that was inhibited by SOD, CAT, or conversion of the cellular hemoglobin to carbonmonoxyhemoglobin, but was not inhibited by LP.

Journal ArticleDOI
TL;DR: It is concluded that [P i ]-independent adenine nucleotide translocation across the inner mitochondrial membrane is not a rate-limiting reaction in oxidative phosphorylation, and the rate of respiration is dependent on extramitochondrial ATP, ADP, and orthophosphate concentrations and not on [ATP/[ADP].

Journal ArticleDOI
TL;DR: Pig heart citrate synthase and mitochondrial malate dehydrogenase interact in polyethylene glycol solutions as indicated by increased solution turbidity, and the observed interaction is highly specific in that neither cytosolic malate dehydration nor nine other proteins showed evidence of specific interaction with either pig heart citrates synthase or mitochondrialmalate dehydration.

Journal ArticleDOI
TL;DR: The incorporation of N -acetyl-[ 14 C]glucosamine into glycolipid was stimulated by exogenous dolichol monophosphate, but inhibited by UMP or tunicamycin, a glucosamine-containing antibiotic.

Journal ArticleDOI
TL;DR: Experimental data for the sedimentation coefficient, while less definitive, were consistent within experimental uncertainty with the same parameters of the worm-like chain model, in substantial agreement with the values derived from small-angle X-ray scattering.

Journal ArticleDOI
TL;DR: The sequence determination was carried out on a mixture of chromatographic forms present in both tissue and pancreatic juice which are identical in amino acid composition, amino-terminal residues, molecular weight, and specific activity, but differ only in asparagine content and susceptibility to enzymatic hydrolysis.

Journal ArticleDOI
TL;DR: Aryl hydrocarbon hydroxylase activity has been demonstrated in the nucleus by a specific histofluorescent technique and aralkylation of DNA, histones, and nonhistone components was demonstrated in these in vitro systems.

Journal ArticleDOI
TL;DR: The double-stranded nature of the deoxyribonucleic was demonstrated by its resistance to degradation by the single-strand-specific S1-nuclease and the density shift in CsCl of +0.016 g/ml upon denaturation.

Journal ArticleDOI
TL;DR: The distribution of the Cu,Zn-superoxide dismutase does not reflect the habitat but, rather, the phylogeny of the organism, and the relation between the oxygen concentration in the atmosphere and the appearance of various forms of superoxide dismUTase during the evolution of photosynthetic organisms is discussed.

Journal ArticleDOI
TL;DR: The much lower ratios required for enhanced inactivation of the complex by pyruvate dehydrogenase kinase than for product inhibition indicate that the level of activity of the regulatory enzyme is not directly determined by the relative affinity of substrates and products of catalytic sites in the pyruVate dehydrogensase complex.

Journal ArticleDOI
TL;DR: The efflux 42K+ from isolated beef heart mitochondria under conditions of near steadystate K+ is increased by repiration and is sensitive to uncouplers and to exogenous Mg2.

Journal ArticleDOI
TL;DR: Almond emulsin contains two kinds of α- l -fucosidases, which could be separated by gel filtration on Sephadex G-200, but one enzyme hydrolyzed Fucα1 → 4GlcNAc and Fuc α1 → 3Glc NAc linkages in milk oligosaccharides, but did not appreciably hydrolyze other fucosyl linkages.