Showing papers in "Archives of Biochemistry and Biophysics in 1986"

TL;DR: Can the superoxide radical exert deleterious effects independent of participating with H2O2 in the production of the hydroxyl radical?

TL;DR: Results indicate that the enzyme oxidizes Mn(II) to Mn( III) and that the Mn(III) complexed to lactate or other alpha-hydroxy acids acts as an obligatory oxidation intermediate in the oxidation of various dyes and lignin model compounds.

TL;DR: Two representative, hydrogen peroxide-dependent extracellular enzymes produced by ligninolytic cultures of the white-rot fungus Phanerochaete chrysosporium BKM-F-1767, were purified and their properties compared.

TL;DR: This study has studied the interaction between three compounds which accumulate in organisms under hydration stress--proline, betaine, and trehalose--and the membrane phospholipids dimyristoylphosphatidylcholine, and found that these compounds increase the area/molecule of these lipids.

TL;DR: Inhibition of the Ca2+-ATPase by oxidative stress is relevant to models of cellular dysfunction in which toxicity is caused by a rise in intracellular calcium.

TL;DR: In addition to being markedly reduced, the Lec13A 4,6-dehydratase activity was relatively insensitive to changes in pH in comparison to the activity in parental cytosol, suggesting that Lec 13A cells might possess a structurally altered GDP-mannose 4, 6-deHydratase enzyme.

TL;DR: Nine independent hybrid clones produced MAbs, either IgG1, IgG2b, or IgM, that bound to purified cytochrome P-450E in radioimmunoassay that should be useful in determining phylogenetic relationships of the BNF- or MC-inducible isozymes and their regulation by other environmental factors.

TL;DR: CRBP, the putative mediator of retinol action, was found to enable retinoic acid to be bound to testicular nuclei, in an interaction similar to the binding of Retinol to liver nuclei described previously.

TL;DR: Two molecular forms of salmon growth hormone (sGH), sGH I and II, have been isolated from the pituitary glands of the chum salmon by a two-step extraction procedure subsequent to acid-acetone extraction, suggesting that they may be genetic variants coded on two separate genes.

TL;DR: The results suggest that alkylation and/or oxidative stress may be important mechanisms in the cytotoxicity of benzoquinone derivatives.

TL;DR: All of the evidence presented in this study indicates that the identical cytochrome P- 450, P-450j, is induced in rat liver by either isoniazid or ethanol, and that this isozyme is closely related to rabbit CytochromeP-450 isozyme 3a.

TL;DR: The results suggest that photoinactivation of catalase generally occurs in leaves under high light intensity, though it is not apparent under normal physiological conditions because it is compensated for by new synthesis.

TL;DR: PFK purified from rabbit skeletal muscle is fully inactivated after being frozen in liquid nitrogen for 30 s and thawed and the addition of ionic zinc to enzyme-organic solute mixtures prior to freezing greatly enhances the cryoprotection imparted by all of the solutes tested.

TL;DR: In a direct comparison with xanthine oxidase from bovine milk, the human enzyme showed a similar specificity toward purine substrates, however, considerable differences between the bovines and human enzymes were observed with nucleoside substrates.

TL;DR: These P-450 species represent a new class of P- 450 isozymes that are important in the metabolism of many endobiotics and xenobiotics.

TL;DR: The sesquiterpene cyclase, trichodiene synthetase, has been purified from a supernatant fraction of Fusarium sporotrichioides by hydrophobic interaction, anion exchange, and gel filtration chromatography and had a specific activity 15-fold higher than that previously reported for preparations of terpenecyclases.

TL;DR: It is suggested that during any physiological process, where there is an energy demand followed by a resting phase, the F1 molecule may follow a "cyclic" path involving the four distinct conformational states of the enzyme.

TL;DR: The results suggest that in these tumor cells, extracellular ATP elicits changes in phosphoinositide metabolism similar to those produced by a wide variety of Ca2+-mobilizing hormones and growth factors.

TL;DR: The reaction kinetics of the peroxidase activity of prostaglandin H synthase have been examined with 15-hydroperoxyeicosatetraenoic acid and hydrogen peroxide as substrates and tetramethylphenylenediamine as cosubstrate.

TL;DR: It is concluded from the high activity of ascorbate peroxidase activity and the levels of antioxidants found that this enzyme can effectively remove low concentrations of peroxides.

TL;DR: Analysis of a Petunia hybrida cell culture resistant to 1 mM glyphosate revealed a 15- to 20-fold increased level of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in the herbicide-tolerant strain, providing further evidence that EPSP synthase is a major site of glyphosate action in plant cells.

TL;DR: The results indicate that the spin trapping agent is able to penetrate well into animal tissues, and to capture radical species under conditions where the latter would be expected to occur.

TL;DR: No effect on either the ability of the adipocytes to synthesize and store or mobilize triacylglycerol was observed, consistent with the hypothesis that other hormones present in crude preparations of TNF acting either alone or synergistically with TNF play a major role in the further metabolic derangements associated with adipose tissue during cachexia.

TL;DR: The present studies suggest that the cardiomyopathy observed with the anthracyclines, and perhaps their antineoplastic activity as well, may be related to their ability to delocalize tissue iron, thereby contributing to the formation of strong oxidants capable of damaging critical cellular constituents.

TL;DR: In this article, the rates of reduction of horseradish peroxidase compound II by p-methoxyphenol (4-hydroxyanisole) have been studied from pH 6.0 to 10.5.

TL;DR: Treatment of immature and adult male rats with 20 xenobiotics that are known inducers of certain cy tochrome P450 isozymes revealed that cytochromes P450f and P450g are relatively refractory to induction, although Kepone appears to be a weak inducer of cytochrome P 450f.

TL;DR: The microsomal enzyme fraction from mung bean seedlings was found to contain glucosidase activity capable of releasing [3H]glucose from the glucose-labeled Glc3Man9GlcNAc, and data indicate that this glucOSidase is a specific alpha-1,2-glucosid enzyme.

TL;DR: Two new cytochrome P-450 forms were purified from liver microsomes of the marine fish Stenotomus chrysops and it is proposed that it is a major contributor to microsomal testosterone 6 beta-hydroxylase activity.

TL;DR: High isozyme specificity in the metabolism of NDMA to an ultimate carcinogen is demonstrated and an important role for cytochrome b5 is suggested in this biotransformation process, indicating a specific effect of intact cyto chrome b5 on the Km,app of the reaction.