Showing papers in "Archives of Biochemistry and Biophysics in 1993"

TL;DR: In this article, the authors used one-electron reduction potentials to predict a pecking order, or hierarchy, for free radical reactions, which is in agreement with experimentally observed free radical electron (hydrogen atom) transfer reactions.

TL;DR: Most cellular reduction of MTT occurs extramitochondrially and probably involves the pyridine nucleotide cofactors NADH and NADPH-dependent mechanisms that are insensitive to respiratory chain inhibitors.

TL;DR: The results suggest that detection of increased oxidation of these probes in endothelial cells is most useful as a marker of a change in general cellular oxidant production.

TL;DR: The differences in amino termini and carboxyl termini of CAPs and CVAPs suggest that the two types of amyloid form by different pathways, on which they encounter different proteases.

TL;DR: In this article, two isoforms of cyclooxygenase (COX) have been identified in eukaryotic cells: COX-1 encoded by a 2.8-kb mRNA, and a mitogen-inducible COX2 encoded by 4-kb.

TL;DR: It is demonstrated that DNase II can mediate internucleosomal DNA digestion characteristic of apoptosis following intracellular acidification, and question the premise that the Ca2+/Mg(2+)-dependent endonuclease is the only end onuclease involved in apoptosis.

TL;DR: The chemical and kinetic properties of the purified phytase P2 points to an identity with an enzyme described by Dassa et al. (1982) as a pH 2.5 acid phosphatase; consequently, this enzyme is a 6-phytase.

TL;DR: It is possible that NO may play a role in the regulation of P450 activity in vivo through differential susceptibility to inhibition by NO for the two O-dealkylase activities.

TL;DR: This report provides the first example of stabilization of proteins during lyophilization through separate, specific treatments of the fundamentally different stresses of freezing and dehydration.

TL;DR: These procedures provide a relatively convenient and reliable means of producing, purifying, and reconstituting a catalytically active and useful derivative of P450 3A4, a human P450 enzyme that has many roles in the oxidation of drugs and other xenobiotic chemicals.

TL;DR: Comparison of the cell and explant cultures under identical pressure conditions suggested that chondrocyte interactions with extracellular matrix are involved in this regulation by cyclic hydrostatic pressure, a finding compatible with the idea of multiple metabolic steps in chONDrocytes, both pre- and post-translational, controlled by the ambient hydrostaticpressure.

TL;DR: This is the first demonstration of the expression of 1C19 at the enzyme level, and correlation studies suggest that 2C19 plays a role in the 4'-hydroxylation of S-mephenytoin.

TL;DR: The infrared spectroscopic technique is a rapid and useful method for studying protein conformation in the dried state and can aid in determining the optimal conditions for stabilization of proteins during lyophilization.

TL;DR: The hypothesis that control of transcription is one primary control mechanism for regulating PGHS-2 expression is supported, as well as the mechanism for glucocorticoid inhibition of PGHS mRNA expression, which is found to depend on transcriptional activation of the gene.

TL;DR: The present results provide a molecular model for an alternative mechanism of lysis that would dominate in cases where the concentration is high enough for hemin to aggregate and aggregates are responsible for the enhancement of permeability and membrane disorder.

TL;DR: Alcohol dehydrogenase is an enzyme with multiple metabolic roles, and the different substrate specificity and tissue localization for each class provide organs and tissues with distinct physiological functions.

TL;DR: The results demonstrate that ONOO- may operate in vivo as a critical macrophage-derived reactive intermediate against T. cruzi, and indicates that classical hydroxyl radical scavengers and metal chelators afforded minimal protection against ONOO(-)-mediated cytotoxicity.

TL;DR: Under these conditions, GSH serves primarily to redox cycle the reactive 1,10-phenanthroline-copper complex and inhibit free radical formation by copper ions in the presence of H2O2, ascorbate, and DNA.

TL;DR: Vignain has properties expected of a cysteine endopeptidase of the papain family, with the exception of a remarkably low reactivity with iodoacetate, and is discussed in relation to the literature on the proteolytic enzymes of legume seeds.

TL;DR: In different transgenic lines, overexpression of SOD by 32-42% above normal had either a minor and/or an insignificant effect on life span of the flies and their ability to withstand experimental oxidative stress, induced by paraquat intake and exposure to hyperoxia (100% oxygen).

TL;DR: The cloning and expression of a cDNA that encodes a soluble epoxide hydrolase from human liver and was expressed in the baculovirus system in the Spodoptera frugiperda 21 cell line was reported.

TL;DR: It is demonstrated that L-14 binds with high affinity to laminin and to relatively long PL chains and indicate that L/N-acetyllactosamine can promote cell adhesion to lamination.

TL;DR: The results indicate that Cu(II) strongly induces the oxidation of HQ and as such may be a factor involved in the oxidative activation and toxicity of HQ in target cells.

TL;DR: This study demonstrates the presence of discrete NAT1 structural variants among Caucasians and unambiguously shows that human NAT1, like NAT2, is a polymorphic locus.

TL;DR: Results show that in vivo vitamin D deficiency impairs cell-mediated immunity and the provision of a vitamin D-sufficient diet for 8 weeks corrected the impaired response of the immune system, while vitamin D administration for 3 weeks did not.

TL;DR: The use of a physiologically balanced medium, i.e., Williams' E formulation, together with an overlay of ECM, preserves PB-induction responsiveness closely resembling that observed in vivo, and should better facilitate mechanistic investigations into the molecular nature of PB induction.

TL;DR: It is shown that norepinephrine (NE), dopamine (DA), dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), serotonin (5-HT), and 5-hydroxyindole acetic acid(5-HIAA) protect brain homogenate and mitochondria against iron-dependent lipid peroxidation and protect brain microsomes against both iron- dependent and iron-independent lipid per oxidation.

TL;DR: Heparin lyases I and III exhibit secondary specificity for oligosaccharides containing an unsulfated alpha-L-idopyranosyluronic acid residue, which is rationalized on the basis of most favorable solution conformation of the uronic acid residues.

TL;DR: Kinetic studies suggest that cellobiose is the preferred substrate and cytochrome c is the Preferred electron acceptor for CDH, and in the absence of these electron acceptors, oxygen serves as a poor electron accepter and is reduced to H2O2.

TL;DR: In this article, transcriptional activation of the prostaglandin G/H synthase isoenzyme 2 (PGHS-2) gene is shown to be responsible for this induction.