Showing papers in "Archives of Biochemistry and Biophysics in 1994"

TL;DR: 5-Aminosalicylate reacts promptly with DPPH, suggesting a potent radical scavenger activity and was found to be the most active in inhibiting Fe2+/ascorbate-induced lipid peroxidation, suggesting an antioxidant activity of chain-breaking type.

TL;DR: It is reported that peroxynitrite exposure to rat heart mitochondria resulted in significant inactivation of electron carriers such as succinate dehydrogenase and NADH dehydrogenases as well as the mitochondrial ATPase, indicating that mitochondria may constitute a key intracellular loci for the toxic effects of peroxysitrite under the various pathological conditions in which each appears to play a contributory role.

TL;DR: It is concluded that catechins and quercetin serve as powerful antioxidants against lipid peroxidation when phospholipid bilayers are exposed to aqueous oxygen radicals.

TL;DR: Three regions of sequence similarity have been reported in several protein and small-molecule S-adenosylmethionine-dependent methyltransferases and it is suggested that these conserved regions contribute to the binding of the substrate S-ADenosyl methionines and/or the product S- adenosylhomocysteine.

TL;DR: A novel proteoglycan (PG) has been identified in culture medium from thin slices of the superficial zone of bovine articular cartilage and may serve as a functional metabolic marker for chondrocytes of the shallow zone of articular Cartilage.

TL;DR: Results suggest that the stimulation of chondrocyte biosynthesis by dynamic mechanical compression at amplitudes up to 10% (stresses up to 0.5 MPa) is related to changes in fluid flow and/or cell shape rather than changes in hydrostatic pressure.

TL;DR: Evaluating directly the P450 form selectivity of TAO, ANF, and DDC establishes that TAO is a selective inhibitor of the human CYP3A subfamily and that DDC cannot be employed as a diagnostic inhibitory probe for CYP2E1.

TL;DR: It is shown that little, if any, of the additional oxygen consumed is converted to hydrogen peroxide, and it is proposed that this occurs via an initial reaction of superoxide with GSH to produce a sulfinyl radical rather than hydrogen transfer to give the thiyl radical.

TL;DR: Results are consistent with agonist-induced peroxynitrite production by endothelial cells and suggests that peroxysitrite may have an important role in oxidant-induced endothelial injury.

TL;DR: The regulatory effects of bF GF and IGF-I on bovine articular cartilage appear age-dependent, and bFGF is capable of promoting either anabolic or catabolic processes, and may therefore serve a dual role in the regulation of cartilage metabolism.

TL;DR: The results suggest that the wax9 gene is expressed at the highest level at the time of maximal synthesis of the surface wax in broccoli, revealing for the first time that a major portion of lipid transfer protein is in the surface Wax.

TL;DR: Comparison of the translated open reading frames to the mature protein sequences gives evidence for post-translational processing at both the amino and carboxyl termini, and protein sequences in the zymogen region support the hypothesis of a cysteine-switch type mechanism of maintaining latency.

TL;DR: The purpose of the proposed nomenclature is to provide for the unambiguous identification of orthologous forms of mammalian FMOs, regardless of the species or tissue in question.

TL;DR: Glutathione was synthesized during initial phases of the respiratory burst, compensating for the amount of glutathione that became protein-bound.

TL;DR: The expressed human P450 1A2 in bacterial membranes was rapidly denatured to cytochrome P420 in the presence of detergents, and the enzyme was catalytically active toward the known substrates 7-ethoxyresorufin and phenacetin.

TL;DR: Results suggest that elevation in the concentration of total reduced thiols in plasma may improve its antioxidant activity under hypoxic/anoxic conditions, and may be particularly useful since other important antioxidant mechanisms such as urate, ascorbate, and vitamin E appear to be inefficient.

TL;DR: In this paper, the properties of spin traps and N-tert-butyl-alpha-phenylnitrone or 5,5'-dimethyl-1-pyroline-Noxide were investigated to protect enzymes against oxidation by uv, hydrogen peroxide/uv, and ozone as determined by the preservation of activity and decreased carbonyl content.

TL;DR: Increased heat sensitivity, ANSA binding, and proteolytic susceptibility are likely related to a decrease in the structural stability of oxidatively modified Glu-6-PDH, and may have important implications for the accumulation of altered protein in vivo.

TL;DR: The present results indicate that Al can stimulate Fe(2+)-supported lipid peroxidation through binding to the membrane and promotion of changes in the arrangement of membrane lipids including packing of fatty acids that will facilitate the propagation of lipidPeroxidation.

TL;DR: The results are consistent with the hypothesis that the order of ouabain affinity between the rat alpha isoforms of the Na,K-ATPase is alpha 3 > alpha 2 >> alpha 1.

TL;DR: The data predict that the invertebrate species examined in this study, which appear to lack an Ah receptor protein like that seen in mammals and fish, may be less sensitive than vertebrates to the effects of environmental contaminants that act through this transcriptional regulator.

TL;DR: Systematic spin-trapping studies of peroxynitrite decomposition in the presence of glutathione established that DMPO-hydroxyl radical adduct formation was metal independent, occurring in a metal-free buffer and being unaffected by diethylene-triaminepentaacetic acid.

TL;DR: Results suggest that the metal site is not readily accessible to solvent, and competitive inhibition by the products L-ornithine and urea indicates a rapid-equilibrium random mechanism for the enzyme.

TL;DR: Inhibitor studies and SDS activation experiments indicate that proteasome is responsible for most of the Hb degradation during exposure of RBC to H2O2, and increased exposure of hydrophobic, and perhaps basic, amino acids is the general common cause for degradation of oxidized proteins.

TL;DR: Evidence is provided that humans may be at higher risk of expressing 1,3-butadiene toxicity compared to mice or rats and that the mouse may be the more appropriate animal model to assess human risk.

TL;DR: The substrate specificity factor of ribulose 1,5-bisphosphate carboxylase/oxygenase from several marine chromophytic and rhodophytic algae is determined, providing further evidence that the properties of this enzyme may evolve or change according to the environment in which the host organism is found.

TL;DR: Results suggest that NADH acting via the outer membrane NADH reductase can catalyze an iron-dependent production of oxygen radicals by rat liver mitochondria, which may play an important role in the development of a state of oxidative stress in the liver by ethanol.

TL;DR: The development of a system for relatively high-level expression of human cytochrome P450 2E1 in Escherichia coli is reported, and the highest levels of expression were found when the first 21 codons of the native sequence were deleted and the Trp immediately following the resulting N-terminal Met was changed to Ala (GCT).

TL;DR: Evidence is presented to refute the hypothesis that lower vertebrates, such as fish, diverged from land animals before this time and are thought to express only a single CYP1 gene and report the isolation and complete genomic nucleotide sequence of two distinct CYP 1 genes in rainbow trout.

TL;DR: In this article, a homo-trimer with a subunit relative molecular mass of 28,500 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was obtained from young leaves of the cassava (Manihot esculenta Crantz).