Showing papers in "Archives of Biochemistry and Biophysics in 1996"

TL;DR: Cytochrome spectra and decreased enzymatic activities in the presence of .NO are consistent with a multiple inhibition of mitochondrial electron transfer at cytochrome oxidase and at the ubiquinone-cytochrome b region of the respiratory chain, the latter leading to the increased O2- production.

TL;DR: Even though nitric oxide can directly cause a transient inhibition of electron transport, the inhibition pattern of mitochondrial respiration observed in the presence of peroxynitrite is the one that closely resembles that found secondary to .NO interactions with intact cells and strongly points to peroxlynitrite as the ultimate reactive intermediate accounting for nitricoxide-dependent inactivation of electron Transport components and ATPase in living cells and tissues.

TL;DR: E Epidemiological studies suggest protective effects of lycopene on some types of cancer, e.g., prostate cancer, and in vitro and in vivo studies on growth of tumor cells support this conclusion.

TL;DR: The results support that peroxynitrite anion rapidly reacts with carbon dioxide to yield an adduct (ONOOCO2-) which can participate in oxidation and nitration processes, thus redirecting the primary reactivity of peroxysitrite.

TL;DR: There is no convincing evidence, however, that products of interaction of HOCl with nucleotides are capable of one-electron oxidation of Fe(CN)6(-4), with generation of free radical intermediates.

TL;DR: Peroxynitrite nitrates tyrosine residues by a mechanism that is catalyzed by CO2 under normal physiological conditions and addition of bicarbonate catalytically enhanced the yield of nitration and overcame the inhibition of these antioxidants.

TL;DR: The findings suggest that the beneficial effects of dietary restriction upon brain function and life span may depend upon its ability to acutely reduce steady-state levels of oxidative stress.

TL;DR: It is proposed that O=N-OOCO 2- rearranges to give a nitrocarbonate anion, O2N-OCO2- which in turn, may serve as the proximal oxidant in biological systems that produce peroxynitrite.

TL;DR: The greatly decreased catalytic efficiency of the I359L variant suggests that leucine homozygotes would eliminate (S)-warfarin, and probably many other CYP2C9 substrates, at much slower rates in vivo than individuals expressing the wild-type enzyme.

TL;DR: The results suggest that the current methods for storing and processing human liver are well suited to preserving microsomal P450 enzyme activity, and that long-term storage of human liver microsomes at -80 degrees C is well suited.

TL;DR: The data show that p38, like ERK, can be activated in neutrophils exposed to an appropriate stimulus, and that some but not all proline-directed kinases are able to participate in the phosphorylation of a protein essential for normal neutrophil function.

TL;DR: Peptidylglutamyl-peptide hydrolase activity remained sensitive to oxidation in protease isolated from young rats, but that from old rats was resistant to oxidative inactivation.

TL;DR: It is demonstrated that NO possesses properties which protect against ROS toxicity and how the use of different NO donor compounds can lead to different conclusions about the role that NO can play in the cytotoxicity of ROS is demonstrated.

TL;DR: The results suggest that the H2O2 generated during the MAO-catalyzed oxidation of biogenic amines and possibly certain neurotransmitters at the outer mitochondrial membrane contributes to the intramitochondrial [H2O 2]ss and may cause oxidative damage to mtDNA.

TL;DR: The results suggest that free radical-induced damage in muscle could be one of the factors terminating muscle effort as the greater antioxidant level should allow trained muscle to withstand oxidative processes more effectively, thus lengthening the time required so that the cell function is sufficiently damaged as to make further exercise impossible.

TL;DR: The reactivities of various antioxidative compounds including catechol derivatives and endogenous radical scavengers toward hydroxyl radical (.OH) were investigated by an electron spin resonance-spin trapping method, thiobarbituric acid method, and DNA strand scission assay.

TL;DR: It is proposed that glutaredoxin is the principal agent responsible for protein dethiolation in vivo, and protein disulfide isomerase and thioredoxin are more effective than glutaredoxins as reductants of insulinprotein disulfides.

TL;DR: It is concluded that the homolytic cleavage of GSNO is efficiently catalyzed by Cu+ and that the GS-*Cu+ complex is catalytically inactive.

TL;DR: It is demonstrated that bovine serum albumin and polyvinylpyrrolidone stabilize lactate dehydrogenase by inhibiting dissociation in the frozen solution, during the initial phase of the sublimation step of lyophilization, and that the holoenzyme is more resistant to the stresses of drying/rehydration than unassociated monomers.

TL;DR: Both nitric oxide and oxygen had larger quenching constants for the undecyl derivative compared to the methylpyrene compound incorporated into erythrocyte plasma membranes, indicating an increased solubility of both gases toward the center of the membrane.

TL;DR: Rat and human UDP-glucuronosyltransferase (UGT) 1.1 are functionally identical and can be considered orthologous enzymes, according to the results of the present study.

TL;DR: To obtain optimum stability of dried rhIL-1ra it was necessary to inhibit conformational change during lyophilization and to store at temperatures below the Tg of the dried formulation, both of which are required to ensure long-term stability.

TL;DR: The results indicate that anthocyanin is bleached by the nonenzymatic reaction with the superoxide radical and suggest that the pigment can function as an antioxidant, and the antioxidative efficiency of Cyanidin to superoxide was 10-fold higher than that of cyanidin-3-sophoroside as a Hibiscus anthcyanin.

TL;DR: A cDNA encoding human P45014DM was isolated from a liver cDNA library using a partial rat lanosterol 14α-demethylase cDNA probe as discussed by the authors.

TL;DR: It is concluded that CYP2C8 is one of the primary, constitutive hepatic arachidonic acid epoxygenases responsible for formation of epoxyeicos atrienoic acids and that cytosolic epoxide hydrolase is the principal liver enzyme which forms the dihydroxyeicosatrienOic acids.

TL;DR: Partial purification of cell cytosol using Sephadex G-75 chromatography showed that zinc-metallothionein (Zn-MT) was induced by 24-h treatment with 100 microM ZnCl2, but the cellular glutATHione content and glutathione peroxidase and catalase activities were unaffected.

TL;DR: It is suggested that b5 is an essential component in P450 3A4-catalyzed testosterone hydroxylation and nifedipine oxidation in human liver microsomes in reconstituted systems.

TL;DR: It is suggested that oxidative stress mediated by peroxynitrite can trigger a critical sequence of events ending in programmed cell death and that intracellular oxidation is a component of the apoptosis of thymocytes, since both oxidative processes and apoptosis can be prevented by Trolox.

TL;DR: Results obtained in the present study favor the view that both iodination and coupling are mediated by the porphyrin pi-cation radical form of compound I, as well as finding that coupling was inhibited in the presence of excess iodide.

TL;DR: In vitro translation of an mRNA coding for elongation factor TuB which was in vitro transcribed from the tufB gene from Escherichia coli is reported, finding that SmP ribosomes translate the TuB mRNA significantly slower than wild-type ribosome do.