Showing papers in "Archives of Biochemistry and Biophysics in 2000"

TL;DR: Iron metabolism, and its coupling with regulation of defenses against oxidative stress, as well as the role played by iron in regulatory protein in sensing redox change, appear as essential factors for life in the presence of oxygen.

TL;DR: The results suggest that CD8 lymphocytes may be a major site of 1,25-dihydroxyvitamin D(3) action, while B lymphocytes are likely not directly regulated by 1, 25- dihydroxvitaminD(3).

TL;DR: The results of the study suggest that the flavonoids and isoflavonoids, similar to cholesterol and alpha-tocopherol, partition into the hydrophobic core of the membrane and cause a dramatic decrease in lipid fluidity in this region of the membranes.

TL;DR: This review examines the consequences of connexin phosphorylation for the regulation of gap junctional communication.

TL;DR: Results point to 8-OH-G as an endogenous source of mutations in eukaryotes and to its likely involvement in the process of carcinogenesis and to a possible role in the prevention of cancer.

TL;DR: EGCG was found to impart differential dose-based NF-kappaB inhibitory response in cancer cells vs normal cells, and suggests that EGCG-caused cell cycle deregulation and apoptosis of cancer cells may be mediated through NF- kappaB inhibition.

TL;DR: Farnesyl diphosphate synthase is identified as the selective target of alendronate in the mevalonate pathway and shows that this enzyme is inhibited by other N-containing bisphosphonates, but not by clodronate, supporting a different mechanism of action for different bisph phosphonates.

TL;DR: Data suggest that injurious compression can stimulate cell death as well as a range of biomechanical and biochemical alterations to the matrix and, possibly, chondrocyte nitric oxide expression within intact cartilage.

TL;DR: If liver mitochondria from obese mice with fatty hepatocytes actually produce increased ROS, this may result from chronic apoptotic stress and provoke adaptations, including increases in UCP-2, that potentiate necrosis.

TL;DR: The therapeutic effects of uric acid may be related to the scavenging of the radicals CO(*-)(3) and NO(*)(2) that are formed from the reaction of peroxynitrite with CO(2) and may represent a new and viable approach for ameliorating the adverse effects associated with peroxlynitrite in many diseases.

TL;DR: This minireview focuses on the three classical catalytic activities of the proteasome, designated chymotrypsin-like, trypsin -like, and peptidyl-glutamyl-peptide hydrolyzing in eukaryotes and also the Activities of the more simple Archaebacteria and Eubacteria proteasomes.

TL;DR: The main feature of age-related potentially dysfunctional alterations in tissues was the development of a shift in activity ratios among different complexes, such that it would tend to hinder the ability of mitochondria to effectively transfer electrons down the respiratory chain and thus adversely affect oxidative phosphorylation and/or autooxidizability of the respiratory components.

TL;DR: It is reported that nitration of tyrosine residue(s) in proteins is sufficient to induce an accelerated degradation of the modified proteins by the proteasome and that the prote asome may be critical for the removal of nitrated proteins in vivo.

TL;DR: A cDNA clone is isolated encoding a putative key enzyme of artemisinin biosynthesis, amorpha-4,11-diene synthase, which has a broad pH optimum between 7.5 and 9.0 and the Km values for farnesyl diphosphate, Mg2+, and Mn2+ are 0.9, 70, and 13 microM, respectively, at pH7.5.

TL;DR: It is reported that the glycosaminoglycan chains of syndecan-4 bind recombinant HepII and it is incorporated into forming focal adhesions.

TL;DR: This minireview focuses on the most important aspects of the sperm acrosome, from its formation during sperm development in the testis to its modification in the epididymis and function following sperm-egg interaction.

TL;DR: Furanocoumarin dimers, GF-I-1 and GF-i-4, were the most potent inhibitors of CYP3A4, suggesting that these compounds are mechanism-based inhibitors of cytochrome P450 (P450) forms.

TL;DR: Results indicate that exogenous methylated arsenic species and endogenous ascorbic acid can cause the release of iron from ferritin, the iron-dependent formation of reactive oxygen species, and DNA damage, which could be a mechanism of action of arsenic carcinogenesis in man.

TL;DR: Conformational and activity analyses indicate that pH changes during freezing in NaP buffer contribute to denaturation of beta-gal, and suggest that proteins formulated inNaP buffer should be frozen and thawed rapidly to minimize exposure to low pH and high buffer salts.

TL;DR: The findings of this study indicate that increased expression of PDK isoenzymes is an important mechanism for bringing about inactivation of the pyruvate dehydrogenase complex during starvation in many but not all tissues of the body.

TL;DR: A combination of nondenaturing electrophoresis and Western blotting let us believe that the high oxidant susceptibility of the 26S proteasome is due to oxidation of essential amino acids in the proteasomesome activator PA 700 which mediates the ATP-dependent proteolysis of the 25S-proteasome.

TL;DR: PIMT is upregulated in neurodegenerative neurons and colocalizes in neurofibrillary tangles (NFTs) in AD and taken together with the enhanced protein isomerization in AD brains, it is implicated that the upregulated PIMT may associate with increased protein isomership in AD.

TL;DR: It has been suggested that abnormalities in copper and H2O2 homeostasis and certain pathological factors functionally similar to the Aβ25-35 could play critical roles in the metal-catalyzed oxidative oligomerization of α-synuclein, which may lead to possible protein aggregation and neurodegenerations.

TL;DR: It is likely that low-barrier hydrogen bonds form at the transition state of any reaction involving general-acid or general-base catalysis, as at that point the pKs of the catalytic group and reactant will be equal.

TL;DR: Laccase produced by Coriolus hirsutus was purified to electrophoretic homogeneity by acetone precipitation, DEAE Sepharose CL-6B, Sephacryl S-200 HR, Hitrap SP, and Mono S chromatography and had highest affinity toward guaiacol.

TL;DR: It is proposed that proanthocyanidins are superior antioxidants as compared to flavon(ol)s proper, whose quinones are more likely to redox-cycle and act as prooxidants.

TL;DR: This review mainly summarizes recent studies on the involvement of these three classes of proteoglycan in cell-cell and cell-substratum interactions during the brain development.

TL;DR: Evidence is provided for a reaction chemistry of quercetin semiquinones with horseradish peroxidase/H( 2)O(2) and GSH ultimately leading to adduct formation instead of to preferential GSH-mediated chemical reduction to regenerate the parent flavonoid.

TL;DR: It is concluded that lycopene affects GJC independent of the formation of acyclo-retinoic acid.