Journal•ISSN: 0006-291X
Biochemical and Biophysical Research Communications
About: Biochemical and Biophysical Research Communications is an academic journal. The journal publishes majorly in the area(s): Receptor & Gene. It has an ISSN identifier of 0006-291X. Over the lifetime, 91794 publication(s) have been published receiving 3158345 citation(s).
Topics: Receptor, Gene, Phosphorylation, Cell culture, Apoptosis
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TL;DR: Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adip onectin is secreted only from adipose tissue.
Abstract: We isolated the human adipose-specific and most abundant gene transcript, apM1 (Maeda, K., et al.,Biochem. Biophys. Res. Commun.221, 286–289, 1996). The apM1 gene product was a kind of soluble matrix protein, which we named adiponectin. To quantitate the plasma adiponectin concentration, we have produced monoclonal and polyclonal antibodies for human adiponectin and developed an enzyme-linked immunosorbent assay (ELISA) system. Adiponectin was abundantly present in the plasma of healthy volunteers in the range from 1.9 to 17.0 mg/ml. Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adiponectin is secreted only from adipose tissue. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of adiponectin in humans.
4,718 citations
4,707 citations
TL;DR: A purified protein derived from the twisted beta-pleated sheet fibrils in cerebrovascular amyloidosis associated with Alzheimer's disease has been isolated and Amino acid sequence analysis and a computer search reveals this protein to have no homology with any protein sequenced thus far.
Abstract: A purified protein derived from the twisted beta-pleated sheet fibrils in cerebrovascular amyloidosis associated with Alzheimer's disease has been isolated by Sephadex G-100 column chromatography with 5 M guanidine-HC1 in 1 N acetic acid and by high performance liquid chromatography. Amino acid sequence analysis and a computer search reveals this protein to have no homology with any protein sequenced thus far. This protein may be derived from a unique serum precursor which may provide a diagnostic test for Alzheimer's disease and a means to understand its pathogenesis.
4,557 citations
TL;DR: The stability and sparing solubility of this chloroglycoluril in water can account for the minimal damage to proteins and living cells observed in these iodinations and allow for elimination of the reduction step employed at the close of iodinations with soluble chloroamides such as chloramine-T.
Abstract: Films of 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril (conveniently “plated” in reaction vessels from methylene chloride solution) react rapidly in the solid phase with aqueous mixtures of I − and proteins to yield iodinated proteins. Similarly applied, this reagent brings about iodination of cell membranes, although, apparently, at a somewhat lesser rate than in iodinations carried out with lactoperoxidase-glucose oxidase. The stability and sparing solubility of this chloroglycoluril in water can account for the minimal damage to proteins and living cells observed in these iodinations; further, these properties allow for elimination of the reduction step employed at the close of iodinations with soluble chloroamides such as chloramine-T.
4,492 citations
3,598 citations