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Showing papers in "Biochemical and Biophysical Research Communications in 1969"


Journal ArticleDOI
TL;DR: Moderate concentrations of thioglycolic acid largely prevented the destruction of tryptophan during acid hydrolysis of proteins.

645 citations


Journal ArticleDOI
TL;DR: No inter ference with cellular function is observed as shown by almost complete regeneration of GSH after incubation with glucose at 370, and no alteration in hemoglobin, osmotic fragility or cell density is found.

507 citations


Journal ArticleDOI
TL;DR: This formulation of structure represents the elucidation of the first of the hypothalamic hormones that have been sought for so long and is chemically based on the chromatographic identity in seventeen diversified systems and biologically based on a quantitative comparison of the hormonal activities of the natural and synthetic products.

495 citations


Journal ArticleDOI
TL;DR: In contrast to the results with the intact enzyme, the reduction of cytochrome c by deflavoxanthine oxidase is not inhibited by erythrocuprein, indicating that the site of production of O2•− in the native enzyme is flavin, rather than non-heme iron.

392 citations


Journal ArticleDOI
TL;DR: Structural studies suggest the possibility that an amino sugar is bound to hemoglobin A1c in diabetic patients.

355 citations


Journal ArticleDOI
TL;DR: The several criteria are discussed which support the idea that the new electrophoretic band, found in histones of non-replicating tissue, is indeed a previously unreported histone.

293 citations


Journal ArticleDOI
TL;DR: The present work, in addition to demonstrating the temperature method for elucidating secondary structure of polypeptides, may be taken as a confirmation of the gramicidin S and valinomycin structures.

268 citations


Journal ArticleDOI
TL;DR: The molecular structure by X-ray crystallography of this cyclododecadepsipeptide as the potassium aurichloride complex is determined, and it is found to be wrapped around the potassium ion coordinated to the oxygen atoms of alternate carbonyl groups, held together by hydrogen bonding, and holding the anion less specifically.

244 citations


Journal ArticleDOI
TL;DR: This species is rapidly destroyed by catalytic quantities of the copper protein, erythrocuprein, and by stoichiometric quantities of ferricytochrome c.

221 citations


Journal ArticleDOI
TL;DR: The 9S RNA fraction of mouse reticulocyte polysomes has been purified and shown to direct the synthesis of mouse hemoglobin β- chains in a mammalian cell-free system under the direction of a mRNA isolated from a different mammalian species.

215 citations



Journal ArticleDOI
TL;DR: DNA polymerase from Escherichia coli was cleaved by limited proteolytic action into two fragments of 76, 000 and 34, 000 molecular weight, which retains the polymerizing activity and the 3′→5′ nuclease activity present in the native enzyme, but not the 5′→3′ nUClease.

Journal ArticleDOI
TL;DR: Hageman factor activation of fibrinolysis appears to be mediated by kallikrein-catalyzed formation of plasmin from plasmineogen, suggesting the formation of a stoichiometric equimolar complex of plAsmin and kallkrein in the course of the activation of pl asminogen by k allikreIn.

Journal ArticleDOI
TL;DR: It is concluded that cyclic AMP is necessary for the normal utilization of a number of carbon sources by E. coli, because it is required for the synthesis of enzymes involved in their metabolism.

Journal ArticleDOI
TL;DR: A Golgi apparatus-rich fraction isolated from rat liver catalyzed the transfer of galactose from UDP-galactose to N-acetylglucosamine with the formation of N-acetylaminolactose as well as the transfer

Journal ArticleDOI
TL;DR: Investigation of the metabolism of vitamin D3 to its biologically active form, 25-hydroxycholecalciferol, in perfused rat livers and in rat liver homogenates found significant conversion was observed, supporting earlier data which indicate that the liver is the major, if not the only, site of conversion of vitaminD3 to 25-HCC.

Journal ArticleDOI
TL;DR: Removal of calcium by EDTA appear to increase PGE stimulation of adenyl cyclase without altering NaF stimulation, confirming the hypothesis that calcium plays a role in regulating the sentivity of cell membranes to hormones or the hormone-like prostaglandins.

Journal ArticleDOI
TL;DR: A protein kinase that is stimulated four- to six-fold by adenosine 3′,5′-monophosphate has been partially purified from rat epididymal adipose tissue and isolated fat cells and it is postulated that the effect of cyclic AMP on this enzyme is related to its effect on lipolysis and/or other processes in adiposa tissue.

Journal ArticleDOI
TL;DR: Mixing experiments suggest that a soluble inhibitor of IM -CoA reductase does not cause the profound drop in activity observed in cholesterol-fed rats, compatible with the existence of a specific, labile,degradative or inactivating protein for HMG-CoA reducing protein.


Journal ArticleDOI
TL;DR: Metal analyses and inhibitor studies have shown that thermolysin, a neutral protease from B. thermoproteolyticus, is a zinc metalloenzyme.

Journal ArticleDOI
TL;DR: The results of the investigation of an infant with abnormalities of both the sulfur amino acids and methylmalonic acid indicate the occurrence of a hitherto unrecognized metabolic abnormality, a defective ability to accumulate the coenzymatically active derivatives of B 12 .

Journal ArticleDOI
TL;DR: When ribosomes are incubated with G factor and GTP, a ribosome-G factor-GDP complex is formed and more complex is isolated in the presence of the antibiotic than in its absence.

Journal ArticleDOI
TL;DR: A solubilized hepatic microsomal enzyme system previously shown to catalyze the w-hydroxylation of fatty acids in liver microsomes led to "solubilization" of the enzyme system by the criterion that enzymatic activity remained in.

Journal ArticleDOI
TL;DR: Cycloheximide blocks the movement of peptidyl-tRNA from acceptor (aminoacyl) site to the donor (peptidyl) site on reticulocyte ribosomes and has no effect on the ribosome dependent GTPase activity of TF-II or on the peptide transferase reaction by which peptides on tRNA in the donor ribosomal site are transferred to an amino acid on t RNA in the acceptor site.

Journal ArticleDOI
TL;DR: The DNA-intercalating dyes ethidium bromide and acriflavin strongly inhibit the enzymatic synthesis of DNA catalyzed by rat liver mitochondrial DNA polymerase, and the mitochondrial enzyme is much more sensitive to these dyes.

Journal ArticleDOI
TL;DR: (Na + + K + )-ATPase from the outer medulla of rabbit kidney was purified to a specific activity of 881 ± 25 moles Pi/mg protein per hr at 37 C.

Journal ArticleDOI
TL;DR: The method appears to be quantitative and resolves individual cytokinins such as the ribosides 6-(3-methyl-2-butenylamino)-9-β d -ribofuranosylpurine (2iPA) and 6-methylthio-9- β- d - ribofuranusylpurines (ms2 iPA).

Journal ArticleDOI
TL;DR: About 90% of the radio-activity in the DNA product after treatment with micrococcal nuclease and spleen phosphodiesterase was released as 3H-cytosine arabinoside, suggesting that most of the incorporation was at the 3′-hydroxyl terminal end and not within the polydeoxynucleotide chain.

Journal ArticleDOI
TL;DR: Evidence found in the literature suggests that inhibition in vitro of P-fructokinase by these compounds plays a role in regulation of glycolysis.