Showing papers in "Biochemical and Biophysical Research Communications in 1972"

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.

TL;DR: It is proposed that the CL of PMN reflects the generation of singlet oxygen, 1 0 2 , which acts per , se , as the bactericidal and/or CL species.

TL;DR: The molecular structures presented have the most probable values of bond-lengths, bond-angles and furanose ring conformations as defined by accurate X-ray crystallographic analyses of relevant monomers.

TL;DR: One of the major proteins present gave a maximal rate of esterification similar to that found with the native lipoprotein, while the other major lipop protein was inactive as a cofactor but significantly reduced activity produced by the cofactor protein.

TL;DR: The method constitutes a powerful new immunochemical technique for the quantitative determination of haptens and reduces the inhibition of enzyme activity in proportion to the quantity of free morphine added.

TL;DR: ApoLp-Ala consistently inhibits LPL when present at levels above 2% of the substrate (w/w), and this inhibition was not overcome by addition of phospholipid, apoLP-Glu, or more enzyme.

TL;DR: It is suggested that the packaging of lysosomal enzymes requires their secretion followed by specific recognition and uptake, and the I-cell mutation would interfere with this process by altering the recognition site on the hydrolases.

TL;DR: Lymphocyte plasma membrane glycoprotiens solubilized in 1% sodium deoxycholate were selectively purified by adsorption to LcH ∗ , covalently attached to Sepharose 4B, and by subsequent elution with methyl-a-D-mannopyranoside, and inhibited the transformation of lymphocytes by LCH and PHA.

TL;DR: The putrescine-activated S-adenosylmethionine decarboxylases of rat ventral prostate and baker's yeast are strongly inhibited by methyl glyoxal bis(guanylhydrazone), a substance which has been reported to exhibit anti-leukemic actions.

TL;DR: The size of the active site of the enzyme Pronase has been determined by the method of diastereoisomeric replacement and shown to be five amino acid residues in length.

TL;DR: Cytochromes P-450 and P-448 were solubilized and partially purified from rat liver microsomes and exhibited different substrate specificities for benzphetamine and 3,4-benzpyrene.

TL;DR: The metabolite was determined in human urine by a method based on the use of the bis (O- 2 H 3 -methyloxime) derivative of dimethyl 7α-hydroxy-5,11-diketotetranor-prostane-1,16-dioate as internal standard and determination of the ratio between unlabeled and deuterium-labeled molecules by multiple-ion analysis.

TL;DR: Cerulenin specifically blocks the activity of β-keto acyl thioester synthetase (condensing enzyme), which may account for the inhibition of overall fatty acid synthesis by the antibiotic.

TL;DR: Preparations of tubulin were examined for their ability to bind vincristine, vinblastine, and colchicine, as measured by adsorption on DEAE impregnated filter paper, and it was found that the ratio of molar binding of col chicine was always twice that of vin Blastine or vincristsine.

TL;DR: Activities of psychosine galactosidase (galactosylsphingosine gaelic hydrolase) were extremely deficient in the brain, liver and kidney of patients with globoid cell leukodystrophy, and the enzyme was similarly deficient in tissues of a fetus affected with this disease.

TL;DR: The reaction of chloroacetaldehyde with adenosine and cytidine produces a fluorescent product in each case, and this reaction should prove extremely useful in nucleic acid chemistry.

TL;DR: A cytoplasmic binding protein (Z protein) has been shown to have a high affinity for fatty acids in rat liver, myocardium, skeletal muscle, intestinal mucosa, adipose tissue and kidney.

TL;DR: A simple, gentle method for radioiodination of proteins has been developed by coupling bovine lactoperoxidase to cyanogen bromide-activated Sepharose-4B, capable of iodinating all proteins studied and was active over wide ranges of protein and KI concentrations as well as pH conditions.

TL;DR: A homologous series of hydrocarbon-coated Sepharoses varying in the length of their alkyl side chains (Seph-NH(CH2)nH) was synthesized, providing a versatile tool for the purification of proteins.

TL;DR: The most probable values of bond-lengths and bond-angles were assigned to the polymer models which also have ribose rings in the standard C3- endo conformation, whereas A-RNA is an eleven-fold helix whereas the A′-RNA helix is twelve-fold.

TL;DR: A variety of evidence indicates that patients with this type of homocystinuria are not deficient in cystathionine synthase activity, and a deficiency of this reductase activity can explain the biochemical abnormalities in these patients.

TL;DR: Comparisons of thyroxine with that obtained by competitive protein binding assay and triiodothyronine with those determined by the previously developed radioimmunoassay afford excellent agreement.

TL;DR: In this paper, the synthesis of prostaglandin E2 from 5,8,11,14-eicosatetraenoic acid (arachidonic acid) by bovine seminal vesicle microsomes can be inhibited by the non-steroidal anti-inflammatory agents indomethacin, naproxen, its enantiomer and aspirin.

TL;DR: It is postulate that in the enzyme-inhibitor-cofactor complex, the 6-carbon of 5-fluoro-2′-deoxyuridylate is bound covalently to the enzyme, with the methylene group covalent linking to the 5- carbon of the nucleotide to either the N-5 or N-10 position of tetrahydrofolate.

TL;DR: An explanation for the discrepancy between the effectiveness of the antibiotics in the two phase system and the transport rates in sarcoplasmic reticulum is provided by the observation of a particularly low affinity of X537A for Ca++ and Sr++ in polar media.

TL;DR: Rat liver microsomes are capable of catalyzing an NADPH-dependent oxidation of epinephrine to adrenochrome that is inhibited by superoxide dismutase that is greater and more sensitive to inhibition by super poisoning at pH 8.5 than pH 7.7.

TL;DR: It is found that two of the reducible components, designated Fraction A, increased with age, and their isolation and identification from mature bovine skin is briefly described.

TL;DR: Tryptic activation of Clostridium botulinum type E progenitor toxin of 147,000 mol.

TL;DR: The oxidation-reduction potential dependence and kinetics of the ESR changes, activated by laser pulses, suggest the signals represent bacteriochlorophyll in the triplet state, which could be a primary intermediate in the early light activated transitions of photosynthesis.

TL;DR: A purified preparation of rat liver microsomal NADPH-cytochrome c reductase has been shown to catalyze the NAD PH-dependent peroxidation of isolatedmicrosomal lipid, suggesting that the superoxide anion, produced by this flavoprotein, is involved in the lipid per oxidation reaction.