Showing papers in "Biochemical and Biophysical Research Communications in 1976"

TL;DR: Two peaks of glutathione peroxidase activity were present in the Sephadex G-150 gel filtration chromatogram of rat liver supernatant when 1.5 mM cumene hydroperoxide was used as substrate, and the second peak represents a second glutathienase activity which catalyzes the destruction of organic hydroperoxides but has little activity toward H 2 O 2 and which persists in severe selenium deficiency.

TL;DR: The data are consistent with the view that N/ Sub 2/O is an obligatory intermediate in the reduction of NO/sub 2//sup -/ to N/ sub 2/ in all of the three organisms studied.

TL;DR: The chemiluminescent response obtained was of high intensity, and could be easily monitored using the standard in-coincidence scintillation spectrophotometry equipment employed in routine isotope counting.

TL;DR: This paper describes the comparative behaviour of two human soft tissue collagens on polyacrylamide gel electrophoresis and describes how this has been applied to the rapid estimation of the relative proportions of the twoCollagens in a range of human skin samples.

TL;DR: Heparin has been fractionated into two distinct forms and the mucopolysaccharide component was clearly separated from the remaining 2 3 of the heparin which could not form a stable complex with antithrombin-heparin cofactor and had minimal anticoagulant activity.

TL;DR: Binding studies by means of molecular sieving and equilibrium dialysis indicates that the drug binds to purified rat brain tubulin in a mole to mole ratio and the conclusion may be drawn that oncodazole is a true microtubule inhibitor.

TL;DR: Outer membrane of Escherichia coli allows a rapid diffusion of saccharides of molecular weights less than 550 and this permeability property could be restored in vesicle membranes reconstituted from isolated phospholipids, lipopolysaccharide, and an outer membrane protein.

TL;DR: Fractionation of pepsin-solubilized collagens from several human tissues has shown that substantial quantities of collagen-like protein remain in solution under conditions leading to the precipitation of Type I, II, and III collagen.

TL;DR: The complete procedure is rapid and isolated myocytes beat, exclude vital stains, have conventional sub-cellular morphology, show tight respiratory coupling and also have a tolerance to external calcium not found in cells isolated by other perfusion techniques.

TL;DR: An interaction between bleomycin and low concentrations of Fe(II) in the degradation of DNA is reported and optical spectral studies reveal that an oxygen-labile complex is formed between bleomerscin and Fe( II).

TL;DR: Lithium was found to produce a marked elevation in the levels of myo-inositol 1-phosphate in the cerebral cortex of treated rats, but this effect was completely inhibited by atropine.

TL;DR: Intravenous injection of sheep antiserum to somatostatin in the rat not only increases basal plasma TSH levels but also potentiates TSH response following exposure to cold, which suggests the involvement of GH-releasing hormone, in addition to som atostatin, in the GH release mechanism.

TL;DR: Soluble extracts of embryonic chick pectoral muscle and myoblast clone L6 agglutinated trypsin treated glutaraldehyde fixed rabbit erythrocytes showed an increase in Agglutination activity, with a three-fold increase in activity of the extracts as the myoblasts fused to form multinucleated myotubes.

TL;DR: A striking increase in the content of an unknown polypeptide was found after cold adaptation, a phenomenon which was reversed during re-adaptation to a normal temperature.

TL;DR: These findings demonstrate that luteinizing hormone receptors are subject to regulation by elevated gonadotropin concentrations, and suggest that receptor loss is initiated by occupancy of a small proportion of the available receptor sites.

TL;DR: Synthetic peptides, representing part of the phosphorylatable site of rat liver pyruvate kinase, were phosphorylated by (32P)ATP and the catalytic subunit of cyclic AMP-stimulated protein kinase.

TL;DR: BP 7,8-diol-9,10-epoxide was approximately 5, 10 and 40 times more mutagenic than benzo[a]pyrene 4,5-oxide (BP 4, 5-oxide) in strains TA 98 and TA 100 of S. typhimurium and in cultured Chinese hamster V79 cells.

TL;DR: The results suggest that the depression of hepatic cytochrome P-450-dependent monooxygenase systems may be a general property of interferon inducing agents.

TL;DR: Despite the differences in age-related changes in specific activity in homogenates of liver, heart and brain the enzyme shows a considerable decline in catalytic activity per antigenic unit in all three organs in both aging rats and mice.

TL;DR: Ten different compounds have been partially purified and characterized and show a different degree of specificity as growth inhibitors of a series of enterobacteria as well as other non-enteric microorganisms.

TL;DR: Competition studies show that this focusyl transferase is the same enzyme as a GDP-flucose:β-N-acetylglucosaminide focusyltransferase previously described to be present in rat liver Golgi apparatus.

TL;DR: It is concluded that TMB-8 inhibits a calcium dependent step in both thrombin- and ionophore-induced secretion, possibly by blocking release of calcium from an internal “trigger” pool.

TL;DR: The results suggest that the Ca2+-sensitive regulatory mechanism of gizzard actomyosin is mediated via a kinase, and the onset of contraction and the resultant increase of the Mg2-ATPase activity is due, at least partly, to the phosphorylation of the 20,000 dalton light chains.

TL;DR: The results indicate that cytochrome P450 acts as an oxene transferase probably involving [FeO] 3+ as the transient intermediate of active oxygen.

TL;DR: Fractionation of the 40–80% (NH 4 ) 2 SO 4 fraction of a soluble rat brain extract on DEAE cellulose resolves three species of enolase activity, two of which react with antiserum to neuron specific protein from rat (NSP-R) and one which does not react.

TL;DR: 2,3-Butanediol is the only compound, other than 1,2-diols, known at present to show a considerable substrate activity, and it is found to serve as substrate for the enzyme, whereas 1, 3-propanediol was not.

TL;DR: The pulse radiolysis technique is used to measure the bimolecular rate constants of this electron-transfer reaction for over 20 nitro compounds, including substituted 2- and 5-nitroimidazoles of interest as antiprotozoal drugs and radiosensitizers, nitrofurans in use as antibacterial agents, and substituted nitrobenzenes previously used as model substrates for nitroreductases.

TL;DR: A number of strains of oral streptococci, previously thought to be virtually lysozyme resistant, have been successfully lysed by a new procedure that isolated high MW DNA and plasmids.

TL;DR: To obtain evidence on the biosynthetic origin of β-MSH, isolated peptides from pituitary in a search for the N- and C-fragments of the prohormone are isolated.

TL;DR: A specific interaction between the O-antigen-containing lipopolysaccharides of Rhizobia and the lectins of their legume hosts has been demonstrated.