Showing papers in "Biochemical and Biophysical Research Communications in 1977"

TL;DR: The experiments suggest that the formation of oxygen radicals followed by lipid peroxidation may be the basis for the cardiotoxic effects of these drugs.

TL;DR: Both phosphodiesterase activator and RBC cytoplasmic activator increase the Vmax and the apparent affinity of (Ca2-Mg2+)ATPase for Ca2+.

TL;DR: A simple procedure for covalently coupling diethylenetriaminepentaacetic acid to proteins and creates hexadentate chelating sites for metal ions on proteins is described.

TL;DR: Results suggest that the ATPase activator is closely related to Ca 2+ binding proteins from a number of sources, and to skeletal muscle troponin-C as well.

TL;DR: Gel filtration chromatography demonstrated the presence of two peaks of glutathione peroxidase activity assayed with cumene hydroperoxide in the soluble fraction of rat liver, brain, kidney, and testis.

TL;DR: Levels of erythrocyte superoxide dismutase, catalase and glutathione peroxidase have been determined in a variety of animal species indicate a primary role for this enzyme in protection of the cell against uncontrolled oxidative processes.

TL;DR: The binding of formylNorleu-Leu-Phe to a site on rabbit neutrophils indicates that a natural bacterial product and synthetic formyl-peptides produce chemotaxis by the same receptor mechanism.

TL;DR: The light-induced changes in pH and ATP level were compared for cell suspensions between strains of Halobacterium halobium differing in pigmentation after growth under the same conditions and the presence of a bacteriorhodopsin different from that in the purple membrane is postulated.

TL;DR: The data suggest that the compound isolated in bovine achilles tendon collagen is a 3-hydroxypyridinium derivative with three amino acid side chains, which is a novel type crosslink of collagen.

TL;DR: By addition of two volumes of a 1M aqueous KOH/dimethylsulfoxide (1585) mixture to the enzymatic incubation medium, it is possible to selectively extract the unmetabolized benzo(a)pyrene in hexane and the radio-activity remaining in the water phase corresponds to all the in vitro synthesized metabolites.

TL;DR: The results provide a possible rationale for the degradation of DNA which accompanies anthracycline administration as adriamycin, daunorubicin and both ring C reduced forms bind intercalatively and completely relax supercoiled DNA.

TL;DR: Findings may represent the converse of agonist-induced desensitization which is associated with decreases in beta-receptor number and provide a potential explanation for the clinically observed “propranolol withdrawal syndrome”.

TL;DR: It is shown that 2-Mercaptoethanol, dithiothreitol, ascorbic acid and xanthine/xanthine oxidase, hitherto commonly used with bleomycin studies, separately cleave DNA by a similar mechanism.

TL;DR: In this paper, the technical details of double antibody radio-immunoassays for the two peptides α and β-endorphin and the specificity characteristics of the antisera were described.

TL;DR: Mouse cerebral guanylate cyclase was activated by catalase in the presence of sodium azide (NaN/sub 3/), which is known to form catalases-NO complex, while nitrosamines and nitric oxide (NO gas) were capable of activating cerebral guANYlate cyclases in the absence of catal enzyme.

TL;DR: Human skin fibroblasts and cells cultured from human arterial smooth muscle produce a platelet-inhibitory prostaglandin in response to mechanical trauma that differs from PGE 1 and PGD 2 and resembles PGI 2 in its stability properties.

TL;DR: Exposure of human blood platelets containing [32P]ATP to collagen fibres or to the divalent cation ionophore, A23187, selectively increased 32P-labelling of polypeptides of mol.

TL;DR: It is suggested that the CH bond of CHCl3 is oxidized by a cytochrome P-450 monooxygenase to produce trichloromethanol, which spontaneously dehydrochlorinates to yield the toxic agent phosgene.

TL;DR: The results suggest that the surface of phagocytes, like that of epithelial cells, contains D-mannose residues which serve for the attachment of certain Gram negative bacteria.

TL;DR: Dihydroalprenolol and dihydroergocryptine were used to measure β and α adrenergic receptors respectively in heart ventricles from control, thyroxine (T 4 )-treated and propylthiouracil (PTU)-treated rats to show an increase in the number of β receptors and a decrease in theNumber of α receptors.

TL;DR: Cyclic AMP may play a crucial role in the regulation of platelet phospholipase acitivity, and this could explain at least in part the inhibition of aggregation caused by substances which, like prostacyclin, raise the levels of cyclicAMP.

TL;DR: This photometabolite was isolated in pure form from endogenous precursors in skins of rats exposed to ultraviolet irradiation; identification as previtamin D3 was based on its ultraviolet absorption spectrum, mass spectrum and its thermal conversion to vitamin D3.

TL;DR: A minor glycopeptide was newly isolated from the exhaustive pronase digest of crystalline ovalbumin by Dowex-50w column chromatography and its structure was determined as GP-VI, which elucidates that the structural requirement of CII enzyme for its substrate is R→2Man α1→3 (R→6) Manα1→6 (R–4) Manβ1–4GlcNAcβ1→4Gl cNAc→Asn.

TL;DR: TRH immunoreactivity has been shown to occur throughout the rat gastrointestinal tract and is destroyed by fresh human serum, and co-chromatographs with TRH on a Sephadex G-10 column and on a SP SephADEx C-25 column.

TL;DR: This finding indicates that fibroblast cell-surface fibronectin, like the fibronECTin purified from plasma, is composed of two high molecular weight polypeptides hed together by disulfide bonds, and suggests that the dimer may in addition form disulfides-bonded multimers.

TL;DR: The results indicate that “serum-sickness” antibodies react with a common disaccharide moiety of non-reducing end of the both glycosphingolipids.

TL;DR: The eluate from the insulin-agarose column demonstrated a major band with an apparent molecular weight of 135,000 on SDS polyacrylamide gel electrophoresis, and because of the possibility of anomalous SDS binding, this molecular weight must be accepted with caution.

TL;DR: More than an elevation of endogenous LH, whether induced by single or repeated injection of a potent LHRH agonist, is capable of down regulating testicular LH receptors with a resultant long-term effect of reduced testicular size and decreased plasma testosterone concentration.

TL;DR: The amino-terminal 37 residues of bovine and human ubiquitin and of the nonhistone component from bovines nuclear protein A24 are identical, indicating that they perform a cell function of basic importance and have a common evolutionary origin.

TL;DR: The cytochrome P-450 content of primary hepatocyte cultures was maintained at levels close to those found in vivo by using a defined medium containing testosterone, thyroxine, hydrocortisone, estradiol, glucagon, insulin, linoleic acid and oleic acid, and results suggest that primary hepatocytes cultures may be a good model of the liver for studying the metabolism and mechanism of action of toxic chemicals.