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Showing papers in "Biochemical and Biophysical Research Communications in 1979"


Journal ArticleDOI
TL;DR: A heptacosapeptide with potent gastrin releasing activity has been isolated from porcine non-antral gastric and intestinal tissue and striking homology in the C-terminal region is seen with bombesin, accounting for the similar bioactivities of the two peptides.

853 citations


Journal ArticleDOI
TL;DR: It is possible that unsaturated DG, which may be derived from PI turnover provoked by various extracellular stimulators, acts as a messenger for activating the enzyme, and that Ca2+ and various phospholipids such as PI and PS seem to play a role cooperatively in this unique receptor mechanism.

657 citations


Journal ArticleDOI
TL;DR: An alkyl ether analog of phosphatidylcholine was prepared from choline plasmalogen of fresh beef heart by reducing the alk-1-enyl moiety to anAlkyl group and by hydrolyzing the sn -2 acyl moieties and replacing it with an acetoyl group.

533 citations


Journal ArticleDOI
TL;DR: Communication between 3T3 and HEL 37 cells was inhibited by inclusion of the tumor promoting agents, 12-0-tetradecanoylphorbol-13-acetate and phorbl-12,13-didecanoate during coculture, and non-promoting derivatives of these phorbol esters did not inhibit transfer of label.

312 citations


Journal ArticleDOI
TL;DR: Results presented herein suggest that the stimulation of phospholipase A 2 by Ca 2+ is also mediated through calmodulin, adding to the growing list of enzymes whose activities are regulated by cal modulin.

301 citations


Journal ArticleDOI
TL;DR: These findings imply further molecular heterogeneity of vertebrate collagens, and the existence of at least 9 different structural genes for collagen chains.

296 citations


Journal ArticleDOI
TL;DR: The substituent of Leukotriene C, a “Slow Reacting Substance” from mouse mast cell tumors, was earlier shown to be a derivative of 5-hydroxy-7,9,11,14-eicosatetraenoic acid with a cysteine containing substitution in thioether linkage at C-6, has been identified as γ-glutamylcysteinylglycine (glutathione).

280 citations


Journal ArticleDOI
TL;DR: Since it has been shown recently that methionine occupies part of the reactive site of α-1-PI, it is possible that the release of myeloperoxidase by leukocytes during phagocytosis inactivates this inhibitor through oxidation of this particular residue, thereby indirectly augmenting the proteolytic activity released by these same cells.

259 citations


Journal ArticleDOI
TL;DR: Stability, monitored by measuring changes in the permeability of liposomes to 6-carboxyfluorescein and sucrose, was found to depend on the cholesterol content ofliposomes and it was promoted by the presence of blood cells.

235 citations


Journal ArticleDOI
TL;DR: Calmodulin from plant, a fungus, porcine brain and the coelenterate, Renillareniformis, were easily purified by the calcium-dependent binding of calmodulin to fluphenazine-Sepharose.

235 citations


Journal ArticleDOI
TL;DR: The binding properties of muscarinic receptors in homogenates of the rat myocardium are altered in the presence of guanine nucleotides, with the most potent nucleotide is Gpp(NH)p, with GTP and GDP both being about ten times less active.

Journal ArticleDOI
TL;DR: The principle and the experimental realization of spin echo correlated spectroscopy (SECSY) are described and its use for studies of proteins is illustrated with 1H n.m.r. spectra of the basic pancreatic trypsin inhibitor.

Journal ArticleDOI
TL;DR: It is concluded that α-neo-endorphin is a “big” Leu-enkephalin, which has never been discovered and its structure shows no relationship with β-lipotropin.

Journal ArticleDOI
TL;DR: Vanadate is a potent inhibitor of the Ca2+-ATPase activity of sarcoplasmic reticulum in the presence of A-23187 and the purified enzyme is sensitive to vanadate even in the absence of the ionophore.

Journal ArticleDOI
TL;DR: Animals pretreated with indomethacin recovered more rapidly and were more active during reperfusion than those without treatment, and PGF2α, 13,14-dihydro-15-keto-PGF2 α, PGE2, and thromboxane B2 increased during reperFusion after brief episodes of ischemia.

Journal ArticleDOI
TL;DR: The reversible, calcium-dependent binding of a calmodulin to phenothiazines has been demonstrated using an immobilized chlorpromazine analog, andCalmodulin has been purified from crude extracts of bovine brain utilizing calcium- dependent binding to Phenothiazine-Sepharose 4B as an initial affinity-based chromatographic procedure.

Journal ArticleDOI
TL;DR: It is observed that Ribavirin triphosphate is a potent competitive inhibitor of the capping guanylation of viral mRNA, which could account for the antiviral potency of the drug against both DNA and RNA viruses and its ineffectiveness against a virus in which the mRNAs derived from them are not capped.

Journal ArticleDOI
TL;DR: 12-HPETE strongly suppresses prostaglandin and thromboxane production by inhibiting platelet cyclo-oxygenase, and its effects of arachidonic acid hydroperoxides exhibit isomeric specificity in platelet homogenates.

Journal ArticleDOI
TL;DR: The specific protection by F-actin of the -COOH terminal region of the heavy chain at the joint 50K-20K against tryptic cleavage and loss of activity suggests that this part of the head can be involved in actin binding site and/or Mg2+ ATP hydrolysis by the acto-S-1 complex.

Journal ArticleDOI
TL;DR: A cyanide insensitive fatty acid oxidation system is detected in human liver and is shown to be localized in peroxisomes by subcellular fractionation in Metrizamide continuous density gradients, supporting its potential contribution to lipid metabolism and to the hypolipidemic effect of Clofibrate and related drugs in humans.

Journal ArticleDOI
TL;DR: The results suggest that TPA does not bind directly to EGF receptors, and interacts synergistically with EGF in stimulating glycolysis and DNA synthesis in 3T3 cells.

Journal ArticleDOI
TL;DR: Activity of enoyl-CoA hydratase in rat liver was elevated about 6-fold by the administration of di-(2-ethylhexyl)phthalate, a hepatic peroxisome proliferator, and almost all of the increased activity was the peroxISomal enzyme, which was distinguished by its heat-lability from mitochondrial one.

Journal ArticleDOI
TL;DR: The DNA from two transplantable hepatocellular carcinoma lines was less methylated than predicted rates of cell division in these tumors, suggesting that an aberration in endogenous DNA methylation may occur during neoplastic transformation.

Journal ArticleDOI
TL;DR: It is suggested that spermine inhibits lipid peroxidation by binding to phospholipids in the microsomes and influencing the activities of NADPH-cytochrome c reductase and NAD PH-peroxidase.

Journal ArticleDOI
TL;DR: Extracts from bovine adrenal medulla and medullary chromaffin granules were found to contain three proteins, 20,000, 10,000 and 5,000 approximate molecular weights which yield tryptic peptides with opioid activity as mentioned in this paper.

Journal ArticleDOI
TL;DR: It is suggested that rat liver microsomes contain glutathione S-transferase distinct from those found in the cytoplasmic and that the microsomal transferase can be activated by modification of microsome sulfhydryl group(s).

Journal ArticleDOI
Robert J. Smith1, Susan S. Iden1
TL;DR: 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate hydrochloride (TMB-8), an antagonist of intracellular calcium, caused a dose-dependent inhibition of lysozyme release from neutrophils exposed to PMA in a calcium-free medium which could be reversed by the addition of calcium to the extracellular medium.

Journal ArticleDOI
TL;DR: Observations indicate that PLC and DG-kinase activities are intimately linked and the activity of PLC may play a central role in mediating platelet function and aggregation.

Journal ArticleDOI
TL;DR: Dioxoheptanoic acid profoundly inhibited ALA dehydratase in broken cell preparations at concentrations as low as 10−7 M and cell growth was markedly inhibited after two cell divisions.

Journal ArticleDOI
TL;DR: The preparation of a highly purified acyl-CoA oxidase from the cell extract of an n-alkane-utilizing yeast, Candida tropicalis, is described, and it can be crystallized from ammonium sulfate solutions without an increase in specific activity, and is homogeneous on ultracentrifuge and disc electrophoresis.