Showing papers in "Biochemical and Biophysical Research Communications in 1980"

TL;DR: The 2D NOE experiment has the principal advantage that it avoids detrimental effects arising from the limited selectivity of preirradiation in crowded spectral regions, and yields with a single instrument setting a complete network of NOE's between all the protons in the macromolecule.

TL;DR: The broadspectrum herbicide glyphosate inhibits the enzymatic conversion of shikimic acid to anthranilic acid in a cell-free extract of Aerobacter, aerogenes 50% at 5 to 7 μM concentrations.

TL;DR: The inhibitory activity binds to actin filaments and reduces their low shear viscosity by up to 99% in a concentration dependent fashion while reducing polymerization to only a minor extent.

TL;DR: A new protein superfamily containing at least three families: ovalbumin, antithrombin-III, and alpha 1 -proteinase inhibitor is proposed, which may still share similarity in gene structure as well as in protein sequence.

TL;DR: The level of acquired thermal resistance of Saccharomyces cerevisiae shows an excellent correlation with the cellular level of the heat shock proteins which are transiently induced by such a temperature shift.

TL;DR: The phagocytosis-enhancing activity of Substance P may play a role in inflammatory processes of neural origin where the involvement of the peptide has been implicated.

TL;DR: Human platelets contain a large amount of Ca2+-activated, phospholipid-dependent protein kinase (protein kinase C), and phosphatidylinositol turnover provoked by thrombin seems to serve as a transmembrane signal for protein phosphorylation during platelet activation.

TL;DR: A plausible site of binding of α-lactalbumin is presented on the basis of the metal-binding site of lysozyme and of the structural models of the protein based on the lyso enzyme structure.

TL;DR: The results suggest that liver Lipase may promote phospholipid removal in vivo and show that a lowering of liver lipase in situ has profound consequences for serum lipoprotein metabolism.

TL;DR: The results suggest that chloroquine and NH4+ inhibit receptor recycling and thereby inhibit ligand uptake in 125I-Mannose-BSA, suggesting that receptors are conserved and recycled.

TL;DR: Comparison of the biological activities of leukotriene E and the 11- trans stereoisomer on guinea pig airways, ileum, and cutaneous microvasculature has revealed a noteworthy dependence of activity on stereochemistry with leukosatetraenoic acid being much more potent in each system.

TL;DR: Benzamide and m -aminobenzamide, which are the most potent inhibitors of poly(adenosine diphosphate ribose) polymerase known, induced many sister chromatid exchanges (SCEs) in Chinese hamster ovary cells CHO-K1.

TL;DR: A distinction between scorpion neurotoxins is proposed according to their binding properties to rat brain synaptosomes because of the 6–10 times more sites for Centruroides than for Androctonus neurotoxin.

TL;DR: The hypothesis that deficient activity of the guanine nucleotide regulatory protein is the molecular basis for hormone resistance in pseudohypoparathyroid patients is supported.

TL;DR: An effective method is described for the almost complete removal of phospholipid from cytochrome oxidase, with a consequent parallel decrease in enzymic activity.

TL;DR: It is demonstrated that two-dimensional (2D) NMR experiments are particularly suitable for studies of biopolymers in H 2 O solution and should further be of particular interest for conformational studies of nucleic acids.

TL;DR: The primary cuts were found to be non-randomly and unevenly distributed through the whole SV40 genome and those located in the “early” half of SV40 DNA were mapped and possible relation of the results to the nucleosomal organization of the compact minichromosome is discussed.

TL;DR: It is proposed that hydroxypyridinium residues are formed by spontaneous interaction of two residues of hydroxylysino-5-ketonorleucine, which provides a novel mechanism for lateral crosslinking within and between fibrils which may account for some of the unique physical properties of hard tissue collagens.

TL;DR: The instantaneous selective action of N-ethylmaleimide suggests that sulfhydryl groups control a K+Cl− transport system which, associated with the low K+ gene, is apparently functionally silent in adult ruminant red cells.

TL;DR: Homogenates of rat basophilic leukemia (RBL-1) cells have a novel lipoxygenase which was stimulated by calcium in a concentration dependent fashion and inhibited by epinephrine, which links this pathway in basophils closely to the release reaction which is calcium dependent.

TL;DR: The 20,000-dalton variant of human growth hormone lacks a sequence of 15 amino acids normally found in the hormone, suggesting that the variant is the product of a deletion mutation in the growth hormone gene.

TL;DR: It is suggested that polymerization of membrane components, subsequent to the peroxidation of membrane lipids, may contribute to the altered biochemical and mechanical properties of aging cells and to their eventual sequestration.

TL;DR: Changes in viscosity are a sensitive index of Mg-ATP dependent shape changes in these membranes and may be due to a membrane bound Ca2+ phosphatase activity which dephosphorylates membranes phosphorylated by a Mg2+ dependent kinase activity.

TL;DR: The results suggest that extracellular Na + and intracellular GTP and Mg ++ may be physiological determinants of epinephrine binding to these receptors.

TL;DR: Incubation in low (0.02 mM)-calcium medium prevented T51B rat liver cells from initiating DNA synthesis, but raising the calcium concentration in the medium from 0.02 to 1.25 mM caused these arrested cells to initiate DNA synthesis 1–2 hours later.

TL;DR: In this article, a rat liver microsomal incubation system containing the free radical spin trap, phenyl-t-butyl nitrone, as well as an NADPH generating system and [13C]CCl4 (90 atom % 13C) produces electron spin resonance spectra consistent with that expected for a trichloromethyl-phenylphenyl-toothbutyl (THB) adduct.

TL;DR: Renal vein plasma has a level that is 20% of arterial plasma indicating that the kidney removes glutathione from plasma not only by glomerular filtration, but also by a non-filtration mechanism that involves γ-glutamyl transpeptidase.

TL;DR: A hydrocortisone stimulated doubling in the rate of β-adrenergic receptor synthesis and incorporation appears to be responsible for this increase in β-receptor concentration in the affected cells.

TL;DR: Using fluorescence histochemical procedures, the antibody can be used to localize type II collagen in sectioned material and the antigenic site of the collagen seems to be located within the helical portion of native molecules.

TL;DR: Leukotriene A was assigned the structure 5(S)- trans -5,6-oxido-7,9- trans -11,14- cis -eicosatetraenoic acid by the enzymatic conversion of a synthetic product of known stereochemistry into the naturally occurring isomer of 5( S),12(R)-dihydroxy-6,8,10, 14-eicOSatetriene acid in human polymorphonuclear