Showing papers in "Biochemical and Biophysical Research Communications in 1981"

TL;DR: Results clearly indicate that 1α,25(OH)2D3, besides its well known biological effect in enhancing intestinal calcium transport and bone mineral mobilization activities, is involved in the cell grwoth and differentiation of HL-60 cells.

TL;DR: It is concluded that unlike the Affi-Gel conjugate, N- cis -aconityl daunomycin-poly(D-lysine) enters cells and reaches the lysosomal compartment, and that the cis -aconsityl spacer releases dauncycin from poly(D -lysine] in the acidic milieu of lysOSomes due to the participation of a free cis -carboxylic group.

TL;DR: Renaturation of Aequorea green-fluorescent protein (A-GFP) was achieved for the first time following denaturation in guanidine-HCl or acid.

TL;DR: Using dephosphorylated β-hexosaminidase as acceptor and [β- 32 P]UDP-N-acetylglucosamine as donor for the phosphate group, phosphorylation of β- hexosaminationidase by microsomes from rat liver, human placenta and human skin fibroblasts was achieved.

TL;DR: The fragmentation pathways observed are defined and the utility in sequence analysis is commented on and a simple acetylation experiment is shown to aid rapid sequence assignment.

TL;DR: Compound R 24571 is found to be a powerful inhibitor of red blood cell Ca ++ -ATPase as well as Ca ++ transport into inside-out red bloodcell vesicles with an IC 50 -value of 0.5 and 2 μM.

TL;DR: Four stereoisomers of 4- methyl-1-[N 2 -(3-methyl-1,2,3,4-tetrahydro-8-quinolinesulfonyl)-L-arginyl]-2-piperidinecarboxylic acid were synthesized and examined for the inhibitory effect on thrombin.

TL;DR: Kinetic analysis suggests that the primary effect of the antipsychotic drugs is mediated through a competitive inhibition of enzyme activation by interacting with phosphlipid.

TL;DR: A group of nine proteins of Escherichia coli K12 vary in steady state level with growth temperature, and are particularly abundant above 40°C, and evidence suggests that this regulatory gene, htpR, makes an activator protein that is required for heat induction of HTP proteins.

TL;DR: N6-(6-aminohexyl)-AMP coupled to diol-silica completely separated a mixture of albumin, lactate dehydrogenase and alcohol dehydrogenases in less than 15 min when the technique of high performance liquid affinity chromatography was employed.

TL;DR: Glycolipid antigen reacting to the monoclonal antibody directed to the developmentally regulated antigen SSEA-1 was isolated from human erythrocytes and colonic adenocarcinoma and a novel polyfucosyl structure locating exclusively at the internal GlcNAc was detected in the tumor antigen.

TL;DR: It is suggested that hepatic lipase may play a role in the interconversion of HDL subfractions.

TL;DR: The findings suggest that some of the side effects of leukocyte interferon therapy may be mediated by opiate receptor binding and provide evidence for a regulatory circuit between the immune and neuroendocrine system.

TL;DR: Results indicate that the peptidoglycan-synthetic enzyme activities of penicillin-binding protein 3 may be involved in the process of cell division.

TL;DR: Substance P, a potent vasodilatory and smooth muscle contracting agent, binds specificially to the formyl peptide receptor on the rabbit neutrophil and stimulates chemotaxis and induces lysosomal enzyme secretion in concentrations which similarily inhibit f Met-Leu-(3H)Phe receptor binding.

TL;DR: Comparison with other data suggest that other free radicals are also likely to react with these compounds; in the case of the hydroxyl radical and DABCO k = 1.25 × 109 M−1s−1 has been determined.

TL;DR: The results suggest that modification of cellular lipids can produce profound effects on both insulin binding and insulin action, and there was an excellent correlation between the inhibition of both insulinbinding and insulinaction by the fatty acids tested.

TL;DR: Isoprene was found to be the main endogenous hydrocarbon of human breath and was also identified in nursing Long-Evans and Sprague Dawley rats, as well as for a short-time post weaning.

TL;DR: The data show that the enzyme is an endopeptidase with a specificity similar to that of a group of microbial proteases, one of which is thermolysin.

TL;DR: 1,25-dihydroxyvitamin D 3 increases serum levels of bone Gla protein (BGP) in rats parallel the previously described effects of the vitamin on BGP secretion by rat osteosarcoma cells in culture.

TL;DR: Two different peptides, which stimulate the respiration of spermatozoa, were purified from the jelly coat of sea urchin, Hemicentrotus pulcherrimus eggs and analysis of the sequence by Edman degradation indicated that those sequences are Gly-Phe-Asp-Leu-Thr-Gly-gly-Gy-Val and Gly- Phe- asp- Leu-Asn-G ly-Gally-Gel

TL;DR: The parasporal crystal produced by a strain of Bacillus thuringiensis var.

TL;DR: The bi-product analogue inhibitor, 2-mercaptomethyl-3-guanidinoethylthiopropanoic acid, has been synthesized in high yield and exhibits a Ki of 2.0 nM with human plasma carboxypeptidase N, making it an ideal compound to study potentiation of bradykinin and other vasoactive peptides.

TL;DR: The activation of tyrosine 3-monooxygenase occurred in a two step reaction, phosphorylation by Ca2+-, calmodulin-dependent protein kinase and activation by activator protein.

TL;DR: Although the physical characteristics of the new anodic ADH forms are similar to those of the known human ADH isoenzymes, the former are not inhibited by 12 mM 4-methyl pyrazole, oxidize ethanol very poorly and appear to prefer longer chain alcohols as substrates.

TL;DR: In this article, the full sequence of α-neo-endorphin has been determined to be : Tyr-Gly-gly-Phe-Leu-Arg-Lys-Tyr-ProLys, in which the 8th residue previously reported as Arg is found to be Tyr.

TL;DR: The data demonstrate a phosphorylation of a serine residue of fructose-1,6-bisphosphatase during this 3 min glucose treatment of glucose starved cells and show a release of the esterified phosphate from the enzyme upon incubation of cells in a glucose-free medium.

TL;DR: Placenta from 10 week's gestation and membrane preparations from a single hydatidiform mole also contained L HRH receptors with similar characteristics to those found in term placenta, the first demonstration of extra-pituitary LHRH receptors in the human.

TL;DR: Purified human T-lymphocytes exhibit 5-lipoxygenase activity as demonstrated by the conversion of arachidonic acid to 5-hydroxy-eicosatetraenoic acid and 5,12-di-HETE isomers of leukotriene B 4 that lack a 6-cis double bond.

TL;DR: The results indicate that the antagonism of calmodulin by phenothiazines is unrelated to their pharmacological specificity and occurs at a structurally-nonspecific hydrophobic site on the protein.