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Showing papers in "Biochemical and Biophysical Research Communications in 1983"


Journal ArticleDOI
TL;DR: Two-dimensional correlated spectroscopy (COSY) is used for measurements of proton-proton spin-spin coupling constants in protein 1H NMR spectra.

2,999 citations


Journal ArticleDOI
TL;DR: The elimination of the dispersive character of the diagonal peaks in phase-sensitive, double quantum-filtered COSY spectra allows identification of cross peaks lying immediately adjacent to the diagonal, which represents a significant improvement over the conventional COSy experiment.

2,254 citations


Journal ArticleDOI
TL;DR: A 28 amino acid peptide with diuretic and natriuretic activity has been purified from rat atrial muscle and is identical to that of atrial natriUREtic factor and cardionatrin I isolated fromRat atria.

740 citations


Journal ArticleDOI
TL;DR: Results confirm that the synthetic pentasaccharide with the above structure corresponds to the actual minimal sequence required in heparin for binding to AT-III.

640 citations


Journal ArticleDOI
TL;DR: An H2O2-requiring enzyme system was found in the extracellular medium of ligninolytic cultures of Phanerochaete chrysosporium and produced ethylene from 2-keto-4-thiomethyl butyric acid and partially degraded 14C-ring labeled lignin.

590 citations


Journal ArticleDOI
TL;DR: The c-Ha-ras gene was hypomethylated in six of eight carcinomas, including five colonic adenocarcinomas and one small cell lung carcinoma, when compared to adjacent normal tissues, the first demonstration of alterations in methylation of cellular oncogenes in human cancer.

498 citations


Journal ArticleDOI
TL;DR: The data appear to present the first direct spectroscopic evidence of turns in a native protein, as seen in Fourier transform infrared spectra obtained in deuterium oxide solution from 1350 to 1800 cm-1.

485 citations


Journal ArticleDOI
TL;DR: A new peptide discovered and isolated from porcine spinal cord by using bioassays for a tachykinin-like effect on the contractility of smooth muscle preparation from guinea-pig ileum has been found to have a remarkable sequence homology to kassinin and substance P, suggesting that neuromedin K may be involved in neural transmission.

427 citations


Journal ArticleDOI
TL;DR: Treatment of isolated melanoma cell plasma membranes with detergent yields a single class of laminin receptor, isolated here for the first time, that could facilitate the interaction of metastasizing tumor cells with the basement membrane.

415 citations


Journal ArticleDOI
TL;DR: The carbohydrate and amino acid composition, and electrophoretic mobility of BSP-2 antigen correspond to those reported for a neural cell adhesion molecule (N-CAM), and methylation analysis of native and neuraminidase treated glycopeptides indicated that the sialic acid occurred as α2–8 bound polysialosyl units, similar to those of the recently described developmentally regulated polysIALosyl glycopePTides of rat brain.

391 citations


Journal ArticleDOI
TL;DR: Matrix Gla protein this article is a 15,000 dalton protein whose amino acid composition includes a single disulfide bond and is not a precursor to bone Gla.

Journal ArticleDOI
TL;DR: Southern hybridisation analysis of DNA isolated from transformants revealed the presence of pBR322 sequences which have integrated into the host genome along with the N. crassa DNA.

Journal ArticleDOI
TL;DR: In this article, structural studies on an amyloid fibril protein of 14 K daltons (AFj(INO)) isolated from a Japanese patient who suffered from familial amymoidotic polyneuropathy were carried out to unambiguously identify its difference from normal human serum prealbumin.

Journal ArticleDOI
TL;DR: A novel decapeptide is purified that exhibits a potent stimulant effect on the smooth muscle preparation of rat uterus and is found to have a surprising sequence homology to amphibian bombesins, suggesting that it may be involved in the neural communication system of mammals as a neuromediator or hormone.

Journal ArticleDOI
TL;DR: Isotope labeling studies showed that 12-oxo-phytodienoic acid is a biosynthetic precursor to jasmonic acid, a plant growth regulator which promotes senescence.

Journal ArticleDOI
TL;DR: A series of experiments with TPA and Ca2+-ionophore indicates that activation of protein kinase C is a prerequisite requirement for release of serotonin, and that this enzyme activation and Ca 2+ mobilization act synergistically to elicit a full cellular response.

Journal ArticleDOI
TL;DR: It is concluded that both the formation of inactive throm boxane A3 as well as the reduction of thromboxane A2 may contribute to the reduced platelet reactivity after dietary eicosapentaenoic acid.

Journal ArticleDOI
TL;DR: Analyses of competitive inhibition of the binding of [3H] leukotriene B4 to neutrophils indicated thatLeukotrienes B4 and B5 were equipotent stimuli of human neutrophil lysosomal degranulation in vitro, as quantified by the release of beta-glucosaminidase.

Journal ArticleDOI
TL;DR: F Forskolin enhances both phases of glucose- induced and of TPA-A23187-induced insulin secretion, which is interpreted in terms of a model of cell activation in which two branches of the calcium messenger system, the calmodulin branch and the C-kinase branch, control the initial and sustained phases of insulin secretion.

Journal ArticleDOI
TL;DR: It is proposed that a ferrous-dioxygen-ferric chelate complex may be the initiating species in lipid peroxidation, and studies with oxy-radical scavengers and catalase suggest that O2-., H2O2, or the .OH are not involved in the initiation reactions.

Journal ArticleDOI
TL;DR: The ability of phorbol 12-myristate 13-acetate to initiate neutrophil responses similar to those produced by the chemotactic factor without causing a rise in the level of intracellular free calcium suggests two possibilities; that there is a second messenger in addition to calcium or that it activates the cells at a point distal to calcium mobilization.

Journal ArticleDOI
Hirofumi Tanaka1, Etsuko Abe1, Chisato Miyaura1, Yoshiko Shiina1, Tatsuo Suda1 
TL;DR: Results clearly indicate that 1 alpha, 25(OH)2D3 induces differentiation of all cell lines examined preferentially along the monocyte-macrophage pathway.

Journal ArticleDOI
TL;DR: In this article, it is suggested that cAMP-dependent reactions maintain low levels of cytoplasmic Ca2+ by promoting transport and binding of Ca2+, which coincides with the reversal of thrombin-induced, Ca2-dependent protein phosphorylation, and cytoskeleton assembly.

Journal ArticleDOI
TL;DR: Various flavonoids were found to be relatively selective inhibitors of arachidonate 5-lipoxygenase which initiates the biosynthesis of leukotrienes with the activity of slow reacting substance of anaphylaxis.

Journal ArticleDOI
TL;DR: A sensitive method is described that separates in nanogram or microgram amounts the heavy chains of immature, fast and slow adult rat muscles in complex mixtures of myosins and reveals the heterogeneous nature of the embryonic myosin heavy chains.

Journal ArticleDOI
TL;DR: Receptor-mediated movement of Tf through acid intracellular compartments provides a mechanism to remove iron from Tf and for apoTf to remain receptor-bound for externalization to the cell surface and subsequent dissociation.

Journal ArticleDOI
TL;DR: Results presented here indicate that out of 23 cell lines derived from diverse types of human tumors, 11 do not grow at all in Met-Hcy+ medium and are absolutely methionine-dependent and 3 grow only slightly in this medium.

Journal ArticleDOI
TL;DR: An increase in the level of intracellular free calcium concentration in rabbit and human neutrophils stimulated by chemotactic factors has been demonstrated directly using the calcium-sensitive fluorescent probe quin-2.

Journal ArticleDOI
TL;DR: The similarity in the induction of synthesis of proteinase inhibitors in tomato cotyledons and of pisatin in pea pods by pecticpolysaccharides and chitosans suggests that the two polysaccharide types may be triggering a similar fundamental system present in peA and tomato plants that regulates the expression of genes for natural protection systems.

Journal ArticleDOI
TL;DR: It is proposed that one way a cell may regulate ribonucleotide reductase activity is by controlling the generation of M2-specific tyrosine free radicals within existing M2 molecules.