Showing papers in "Biochemical and Biophysical Research Communications in 1984"

TL;DR: A purified protein derived from the twisted beta-pleated sheet fibrils in cerebrovascular amyloidosis associated with Alzheimer's disease has been isolated and Amino acid sequence analysis and a computer search reveals this protein to have no homology with any protein sequenced thus far.

TL;DR: The cerebrovascular amyloid protein from a case of adult Down's syndrome was isolated and purified and Amino acid sequence analysis showed it to be homologous to that of the beta protein of Alzheimer's disease.

TL;DR: Mammalian cells were after irradiation suspended in melted agarose, and casted on microscope slides, and DNA had migrated towards the anode and this migration was more pronounced in irradiated than in control cells.

TL;DR: Results suggest that hepatotropin is a new growth factor that did not stimulate DNA synthesis in cultured hepatocytes and was additive with that of insulin plus epidermal growth factor.

TL;DR: The neurotoxic chemical MPTP is metabolized by rat brain mitochondrial fractions at a rate of 0.91 +/- 0.02 nmoles/mg protein/min, and the major metabolite has been identified as the 1-methyl-4- phenylpyridinium species.

TL;DR: A survey for natriuretic factors in human atrial extract was performed by using in vitro assay for the relaxant effect on the contractility of chick rectum.

TL;DR: Treatment of intact 3T3 cells with biologically active phorbol esters causes a progressive decline in the total activity of Ca2+-sensitive, phospholipid-dependent protein kinase (protein kinase C) measured in cell-free, detergent-solubilized, extracts.

TL;DR: While H-7 inhibited the protein kinase C-mediated phosphorylation in 12-0-tetradecanoylphorbol-13-acetate (TPA)-stimulated platelets, this compound did not block the Ca2+-calmodulin-dependent phosphorylations in Ca2- ionophore A23187-stimulated cells.

TL;DR: A rapid and reproducible radioimmunoassay method was developed for rat atrial natriuretic factor (ANF)-IV and revealed high and low molecular weight ANF in atria and hypothalamus while only a low molecular Weight form was found in plasma.

TL;DR: During the course of ANF purification, the ANF-induced increase of cGMP production by kidney cells paralleled the heightened specific natriuretic activity of the atrial factor, and indirect inhibition of c GMP phosphodiesterase appears to participate in its effect.

TL;DR: Effects of inositol 1,4,5- trisphosphate, extracted from human erythrocyte ghosts, on Ca2+ release from intracellular store sites were studied in saponin-treated single muscle cells of the porcine coronary artery and this agent may play the role of messenger in increasing the cytosolic Ca2+, provoking pharmaco-mechanical coupling, and thus producing the contraction.

TL;DR: Native γ-trace, a small basic protein present in high concentration in cerebrospinal fluid, semen and neuroendocrine cells, but of unknown biological function, is shown to be a potent inhibitor of the cysteine proteinases papain, ficin, and human cathepsins B, H and L.

TL;DR: Evidence is presented that tamoxifen is an antagonist of calmodulin, a major cellular calcium receptor and calcium dependent regulator of many cellular processes and inhibited the activation of phosphodiesterase by Calmodulin.

TL;DR: The ring structure formed by the disulfide linkage between the two half-cystine residues was found essential for both the natriuretic activity and smooth muscle relaxant activity.

TL;DR: The alkaline transfer offers a higher hybridization efficiency and simplifies the transfer procedure as compared with the standard method of DNA transfer at neutral pH, and can be used to remove RNA contamination from the transferred DNA.

TL;DR: The data suggest that the activation of C-kinase plays a significant role in regulating vascular smooth muscle contraction, and this pathway may be involved in this process.

TL;DR: The structure of the major tetraene was established by physical methods as well as by chemical degradation and found to be 5,6,15L-trihydroxy-7,9,11,13-eicosatetraenoic acid.

TL;DR: Inositol triphosphate is the second messenger of stimulus-secretion coupling in atrial cells, and is suggested to be the cause of liberation of atrial natriuretic factor.

TL;DR: Results provide evidence that cysteine proteinases, possibly lysosomal cathepsins, are necessary for bone resorption.

TL;DR: A potential role for protein kinase C in regulating beta-adrenergic receptor function is suggested in regulatingalpha-phorbol diesters or catecholamines and in promoting adenylate cyclase desensitization in duck erythrocytes with tumor promotion.

TL;DR: Two cockroach neuropeptides are assigned by application of fast atom bombardment mass spectrometry, including high resolution and linked scan (metastable) studies, and show considerable homology with two other invertebrates, adipokinetic hormone and red pigment concentrating hormone.

TL;DR: Another decapeptide is purified that exhibits a potent stimulant effect on the smooth muscle preparation of rat uterus and is found to be identical with the carboxy-terminal subsequence of gastrin releasing peptide, and to display a potent contractile activity on rat uterus in the characteristic manner of bombesin.

TL;DR: An interesting pattern in the genetic code has been discovered: Codons for hydrophilic and hydrophobic amino acids on one strand of DNA are complemented by codons for Hydrophobic and Hydrophilic amino acid on the other DNA strand, respectively.

TL;DR: MPTP appears to be a suicide inactivator of MAO, which is oxidized by brain mitochondrial preparations in a process which is blocked by deprenyl and pargyline, implying catalysis by monoamine oxidase B.

TL;DR: Since TPA activates phospholipid-dependent protein kinase C in a manner analogous to endogenous diacylglycerol, a critical role for the latter in α-adrenergic events is suggested.

TL;DR: The data indicate that the specific receptor for ANF is present in VSMC and suggest that intracellular cyclic GMP may be involved in its vasorelaxant effect.

TL;DR: 1-Oleoyl-2-acetyl-glycerol, which activates Ca2+-activated phospholipid-dependent protein kinase, induced the superoxide-production of human neutrophils, while other diacylglycerols did not, indicating the possible involvement of the kinase in the induction process.

TL;DR: The experiments reported here provide a possible molecular mechanism for theactivated macrophage cytotoxic effect and are significant that mitochondrial respiration and DNA replication, both strongly inhibited in target cells by activated macrophages, are metabolic pathways with enzymatic activity vulnerable to inhibition by depletion of intracellular iron.

TL;DR: The activity of peroxisomal enzymes was studied in human liver and cultured human skin fibroblasts in relation to the finding that morphologically distinct peroxISomes are not detectable in patients with the cerebro-hepato-renal (Zellweger) syndrome.

TL;DR: The Zellweger syndrome constitutes the first inborn error of metabolism with a deficiency in an enzyme involved in phospholipid biosynthesis, and the findings suggest a method for prenatal diagnosis of this disease.