Showing papers in "Biochemical and Biophysical Research Communications in 1985"

TL;DR: The results demonstrate that the amyloids associated with chronic hemodialysis contains as major component a new form of amyloid fibril protein that is homologous to beta 2-microglobulin.

TL;DR: It is shown that DNA can be extracted from tissues prepared for routine histopathological examination and it is double stranded, cleavable with restriction endonucleases, and suitable for a variety of standard techniques used in molecular biology.

TL;DR: Bovine follicular fluid was used as a source for the isolation of gonadal inhibin, the activity of which was monitored by the dose dependent suppression of the FSH content of cultured pituitary cells.

TL;DR: The results demonstrate the presence of FGF in these cells and suggest that at least one of the hitherto unidentified mitotic activities in these extracts is due to a mitogen indistinguishable from FGF.

TL;DR: Rechromatography of the latent beta-TGF-containing fractions under acidic conditions converts the high molecular weight form to an apparent 16 Kd active form, which is suggested to correspond to a complex of a beta- TGF associated with a carrier or binding protein.

TL;DR: It is reported here that MDA-468, a human breast cancer cell line with a very high number of EGF receptors, is growth-inhibited at EGF concentrations that stimulate most other cells.

TL;DR: Purification of ovarian inhibin from porcine follicular fluid was performed by using an bioassay based upon the suppression of spontaneous FSH release from cultured cells of rat anterior pituitary to specifically suppress the secretion of FSH, but not of LH from the pituitsary cells.

TL;DR: Findings indicate that TGF-beta is a strong growth inhibitor of adult rat hepatocytes and may block their shift from the G1 phase to the S phase and the physiological role of T GF-beta in inhibiting growth of adult hepatocytes during liver regeneration is discussed.

TL;DR: Findings provide direct evidence for Ca2+-ryanodine receptor complexes that may play a role in excitation-contraction coupling in rabbit skeletal and cardiac muscle.

TL;DR: Antibodies to residues 1-6 were raised which could immunoneutralize purified inhibin activity in an in vitro assay and showed one band on SDS PAGE and in the same experiment two bands after reduction suggesting a molecular weight of 32K for inhibin.

TL;DR: Evidence from three types of experiments indicates that platelet activating factor (PAF)1 is an important mediator of endotoxin-induced hypotension in rats, and these endot toxin-refractory rats do respond to subsequent PAF infusions.

TL;DR: Two novel peptides eliciting a potent stimulant effect on the rat uterus smooth muscle have been purified and identified in porcine spinal cord and identified as neuromedin U-8 and U-25 referring to their uterus stimulating activity.

TL;DR: A 36 amino acid residue peptide, which contains a substance K sequence at its C-terminus has been isolated from porcine brain extracts and is highly biologically active with regard to gallbladder contraction, protein extravasation, hypotension and bronchial smooth muscle spasm.

TL;DR: Mouse brain synaptosomal preparations were used to study uptake of N-methyl-4-phenylpyridine (MPP+), a metabolite of the neurotoxin MPTP, and this selective uptake may contribute to the specificity of the toxic effects of MPTP on nigrostriatal dopaminergic neurons.

TL;DR: Findings indicate that the formation of polyubiquitin chains is not obligatory for protein breakdown, though it may accelerate the rate of this process.

TL;DR: Six recombinant pBR322 plasmids which contain apoaequorin cDNA sequences are isolated and it is reported that this cDNA is apparently full-length.

TL;DR: It was revealed that ANP immunoreactivity in human atria is composed of alpha-(28 residues), beta-(56 residues: alpha-h ANP dimer) and gamma-hANP(126 residues) in various ratios; in rat, porcine and bovine atrial tissues, however, gamma-rANP was found to be a major component.

TL;DR: It is shown for the first time that normal human pulmonary alveolar macrophages (PAM) markedly enhance their basal rate of the production of [3H]-1,25(OH)2D3 when cultured in the presence of recombinant gamma-interferon (gamma-IFN).

TL;DR: The results indicate that alpha-hANP is the predominant form of alpha- hANP-LI in human plasma and that this form generated in the atrial cardiocytes is preferentially released from these cells and circulates in the body.

TL;DR: Venous plasma levels of neuropeptide Y-like immunoreactivity increased in parallel with catecholamines, heart rate and blood pressure during graded physical exercise in man, suggesting release of a neural origin.

TL;DR: The amino acid sequence of bovine brain acidic FGF has a 53% absolute homology with that of bovo pituitary basic FGF suggesting that these endothelial cell mitogens are derived from a single ancestral gene.

TL;DR: An acidic glycolipid antigen that reacts with monoclonal IgM in patients with demyelinating neuropathy and with the mouse monOClonal antibody, HNK-1, was purified from human peripheral nerves and suggested the following structure with a sulfate in a position that remains to be determined.

TL;DR: It is proposed that this complex of receptor-guanine nucleotide regulatory protein-phospholipase C may be applicable to the class of receptors which mobilize intracellular Ca++ by stimulating polyphosphoinositide breakdown.

TL;DR: This work has determined the DNA sequence for a cDNA for the 26-kDa class of murine amelogenin and deduced its corresponding amino acid sequence, which is homologous to bovine or porcines extracted from developing enamel matrices.

TL;DR: Results show that rat atrial cells secrete a low molecular weight natriuretic peptide which is highly active, but store the less active large molecular weight form(s).

TL;DR: Removal of the biologically active heparinlike components from endothelial cells with 0.05% trypsin suggests that these molecular species are present on the cell surface.

TL;DR: In rat aortic smooth muscle cells in culture, calcitonin gene-related peptide stimulated cAMP formation in a dose-dependent manner, half-maximally effective at 0.5 to 1 nM, probably a mediator of CGRP action in vascular smooth muscle.

TL;DR: The results obtained suggest very active synthesis of prealbumin in choroid plexus, which would be an important link in the transport of thyroid hormones from the blood to the brain via the cerebrospinal fluid.

TL;DR: Results clearly show that there exists a widespread neural system containing ANPs in the head using a specific radioimmunoassay for alpha-atrial natriuretic polypeptide.

TL;DR: Yeast mutants deficient in activities of cytosolic superoxide dismutase and catalase A and T were exposed to four different kinds of oxygen stress and the results suggest that superoxide radicals are directly responsible for cytotoxic effects of oxygen.