Showing papers in "Biochemical and Biophysical Research Communications in 1986"

TL;DR: Staurosporine, microbial alkaloid which has been known to have antifungal activity was found to inhibit markedly phospholipid/Ca++dependent protein kinase (protein kinase C) from rat brain, with an IC50 value of 2.7 nM.

TL;DR: The authors have isolated amyloid fibrils from an insulin-secreting human tumour and purified the fibrillar protein, which has about 50% homology with the neuropeptide CGRP (calcitonin gene related peptide).

TL;DR: It is shown that human KB carcinoma cells which express the mdr1 gene also express P-glycoprotein, and that cDNAs encoding P- glycoprotein cross-hybridize with m dr1 cDN as, Thus, the mDr1 gene codes for P- Glycoprotein.

TL;DR: The effect of chlorate on protein sulfation in intact cells was investigated and chlorate treatment provides a powerful tool for studying the biological significance ofprotein sulfation.

TL;DR: The data suggests that tumor necrosis factor may be an important immunopotentiating agent in addition to its previously described cytolytic and metabolic activities.

TL;DR: PGE2 may be a potent autoregulatory factor that dramatically influences tumor necrosis factor production and 10(-7) molar PGE2 reduced tumor necrot factor production to basal levels.

TL;DR: Distributional patterns and developmental changes may be the result of differences in the ability of various tissues to dispose of an extraneous metabolite, or, reflect alterations in a specific functional requirement for D-aspartic acid.

TL;DR: The complete amino acid sequences of two forms of human ovarian inhibin have been determined through cloning and nucleotide sequencing of cDNAs encoding their individual subunit precursors.

TL;DR: The results demonstrate that TGF beta and FGF can interact at the cellular level to modulate growth and suggest that many of the biological activities of FGF observed in vitro and in vivo may be regulated by the presence of TGF Beta and related proteins in the local cellular milieu.

TL;DR: It is reported that tumor necrosis factor (TNF) provokes superoxide anion generation from human neutrophils and movements of intracellular calcium may mediate the TNF-stimulated superoxideAnion generation.

TL;DR: 18-alpha-glycyrrhetinic acid (AGA) was found to inhibit communication by more than 95% at concentrations as low as 2 microM and has properties of a useful inhibitor in the study of intercellular junctional communication.

TL;DR: High concentrations of FGF are ineffective in overcoming TGF-beta-induced inhibition of cell proliferation, suggesting that antagonism of growth factor-induced cell proliferation by TGF -beta is of a noncompetitive nature.

TL;DR: The discovery of an uptake system for MPP+ in mitochondria which is greatly potentiated by the presence of malate plus glutamate and inhibited by respiratory inhibitors, suggesting an energy-dependent carrier is reported here.

TL;DR: Results indicate that astroglial cells cultured from mouse brain are able to synthesize and secrete beta NGF in culture.

TL;DR: A 34,000-dalton protein isolated from the heat-soluble fraction of avian smooth muscle using the procedures of ammonium sulfate fractionation, cation exchange chromatography and gel filtration bound to F-actin and F- actin-tropomyosin in a Ca2-independent manner, but it Ca2+-dependently interacted with calmodulin.

TL;DR: It is suggested that beta-Transforming Growth Factor is stored in human blood platelets as a poorly active high molecular weight complex which may be dissociated and activated in appropriate in vivo microenvironments.

TL;DR: 4-Phenyl-N-methylpyridinium (MPP+), the oxidation product of the neurotoxic amine MPTP, is considerably more inhibitory to the oxidation of NAD+-linked substrates in intact mitochondria in State 3 than is 4-phenylpyridine, which causes efflux of MPP+ from the mitochondria and a reversal of its inhibitory action.

TL;DR: Data suggest that as little as a 17 residue sequence of beta-protein may be required to form fibrils and that the complete sequence of the 4 kDa beta- protein may be important in determining insolubility and the formation of intermediate size polymers.

TL;DR: Results of the present study suggest that arachidonic acid derived oxygenation products, in particular lipoxin A, may serve as intracellular activators of protein kinase C.

TL;DR: In this paper, DNAs from Mycobacterium tuberculosis, M. phlei and M. smegmatis were digested by restriction enzymes and hybridized with three probes consisting of the 5' (16S rRNA), the middle (16 s and 23S rRNB), and the 3' (23S and 5S r RNB) portions of the Escherichia coli rrnB operon.

TL;DR: The findings suggest that inhibin and TGF beta, two closely related molecules, play novel and opposite roles in modulating the follicular functions.

TL;DR: The results show that electron transfer from ubisemiquinone to oxygen depends on the availability of protons, and suggest a role of cytochrome b 566 in mitochondrial O2 formation.

TL;DR: The exposure of human granulocytes to the tumor promoter, tetradecanoylphorbolacetate (TPA), resulted in the accumulation of 8-hydroxydeoxyguanosine (8-OHdG) in the DNA of the treated cells, which was quantitated at subpicomole levels utilizing high pressure liquid chromatography with electrochemical detection (LCED).

TL;DR: Three distinct polypeptides are resolved which are demonstrate to correspond to type 1, 2A and 2B myosin heavy chain isoforms by using specific monoclonal antibodies.

TL;DR: The sequence homology between the leucosulfakinins and the vertebrate hormones, as well as their analogous myotropic activity, suggest that gastrin/cholecystokinin-like neuropeptides are not confined to vertebrates, but also occur in invertebrates.

TL;DR: The 'psoriasis derived' compound was shown to be stereochemically distinct from the platelet 12(S)-enantiomer as its derivative co-chromatographed with the 12(R)-diastereomer.

TL;DR: The functional role of this 5 kDa proteolipid apoprotein is a functionally important constituent of lung surfactant as measured with a pulsating bubble surfactometer.

TL;DR: It is proposed that this second FSH-releasing substance present in porcine follicular fluid is a homodimeric protein composed of two β-subunits of inhibin A joined together by disulfide bond(s).

TL;DR: The monoamine oxidase inhibitor pargyline provided significant protection against MPTP-induced cell death and ATP loss, but had no effect with MPP+.

TL;DR: Protein kinase C was purified from rat brain cytosol by using a high performance liquid chromatography (HPLC), Pharmacia FPLC system and was nearly pure by silver staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.