Showing papers in "Biochemical and Biophysical Research Communications in 1988"

TL;DR: The results suggest that nitric oxide is the precursor of nitrite/nitrate synthesized by cytotoxic activated macrophages and, via formation of iron-nitric oxide complexes and subsequent degradation of Iron-sulfur prosthetic groups, an effector molecule.

TL;DR: Data indicate that L-arginine is the physiological precursor for the formation of NO which mediates endothelium-dependent relaxation.

TL;DR: Pentoxifylline, a methylxanthine, is efficacious in suppressing LPS-induced MO-derived TNF at the level of both TNF mRNA accumulation and TNF supernatant bioactivity, and the mechanism for this suppressive effect is via the generation of intracellular cAMP.

TL;DR: The results suggest an autocrine role for BP-53 in regulating fibroblast responsiveness to IGF-I, and appear to act by increasing the maximal rate of thymidine incorporation rather than the sensitivity of this process to IGF -I.

TL;DR: A cDNA clone was isolated, MK1, whose RNA level increased in early stages of retinoic acid-induced differentiation of embryonal carcinoma cells, and the amount of MK1 RNA progressively decreased in the later stages of the differentiation.

TL;DR: To test the role of topoisomerase in the transformation by oncogenes, the effect of genistein on the transformation of NIH 3T3 cells by transfection with [Val 12]Ha-ras was compared with that of N-alpha-tosyl-L-lysyl-chloromethyl ketone (TLCK), since Genistein inhibits tyrosine kinase as well as TLCK.

TL;DR: It is suggested that interleukin-6 stimulates the secretion of adrenocorticotropic hormone through the corticotropin-releasing hormone and is possibly involved in the interaction between the neuroendocrine and immune system.

TL;DR: The present data clearly demonstrates the presence of specific receptors for pET distinct from those of the well-recognized vasoconstrictors and voltage-dependent Ca2+-channels in cultured rat VSMC, and suggest that pET-induced increase in [Ca2+]i is involved in the mechanism of its vasoconStriction.

TL;DR: Evidence is provided that a cDNA that encodes a major part of the human androgen receptor is isolated from a library prepared of mRNA of human breast cancer T47D cells with an oligonucleotide probe homologous to parts of the region encoding the DNA-binding domain of steroid receptors.

TL;DR: The incomplete absorption of eicosapentaenoic and docosahexaenoic acids from fish oil triacylglycerols correlates well with known in vitro pancreatic lipase activity.

TL;DR: Concentrations of NO formed by stimulated EC were quantitatively sufficient to fully explain the smooth muscle relaxation determined in the bioassay and provide convincing evidence that under basal, BK and ATP-stimulated conditions nitric oxide is solely responsible for the vasodilatory properties of EDRF.

TL;DR: It is shown that endothelin stimulates the metabolism of inositol phosphates and induces the mobilization of intracellular Ca2+ stores and this mechanism of action explains why part of the endothelins-induced vasocontriction is eliminated by L-type Ca2+, channel blockers.

TL;DR: It is concluded that NE or GTP-γ-S causes an increase in myofilament Ca 2+ sensitivity and that G protein may be involved in receptor signal transduction system.

TL;DR: PET in combination with tetradecanoyl-phorbol-acetate resulted in a synergistic effect on IR-rANP secretion, suggesting that ET may play as an endogenous secretagogue for rANP by modulating Ca2+ influx through the voltage-dependent Ca2+.

TL;DR: The purified IGF-BP protein has an RGD sequence near its C-terminus, which may account for its ability to attach to cells and to potentiate the biological actions of IGF-I.

TL;DR: A 99-amino acid HIV-1 protease, produced by chemical synthesis or by expression in bacteria, is shown here to hydrolyze peptides corresponding to all of the known cleavage sites in the HIV- 1 gag and pol polyproteins.

TL;DR: Results suggest that autophagically sequestered lactose and endocytosed beta-galactosidase were delivered to the same prelysosomal vacuole, where the lactose was hydrolysed by the enzyme.

TL;DR: An amino acid sequence of human prepro-BNP of 134 residues has been deduced, in which a minimum bioactive unit highly homologous to porcine BNP-32 is present at the carboxy-terminus.

TL;DR: Calpeptin was found to be a useful cell-penetrative calpain inhibitor and inhibited 20K phosphorylation in platelets stimulated by thrombin, ionomycin or collagen.

TL;DR: Calcium store of the skinned fibers of the guinea-pig portal vein, pulmonary artery and taenia caeci lost its capacity to hold Ca and changes in the agonist-evoked contraction of intact muscle due to the ryanodine treatment suggest that agonists release Ca from S alpha which produces the initial phase of contractures.

TL;DR: It is suggested that IL-6 plays a key role in the stimulation of synthesis of serum amyloid A and C-reactive protein by the human liver cells.

TL;DR: Gene-recombinant human growth hormone elicited a dose-dependent stimulation of the proliferation of osteoblast-like cells when grown in strictly serum-free longterm cultures, and direct stimulation of OB proliferation is, at least in part, mediated by IGF I-like immunoreactivity.

TL;DR: The observation that chronic infusions of basic FGF can elicit a repair response at the site of injury suggests that this growth factor may have therapeutic applications that extend beyond its capacity to induce neovascularization.

TL;DR: Half-maximally effective concentrations of endothelin for all these cellular responses were comparable and not significantly different between the vascular cell isolates and the involvement of the phospholipase C-signal transduction pathway in mediatingendothelin-induced vasoconstriction is invoked.

TL;DR: Its hydrolytic stability and its ability to inhibit the infectivity and replication of HIV in T-cells at concentrations of approximately 200- to 400-fold below toxic concentrations make carbovir a top-priority candidate for development as a potential antiretroviral agent in the treatment of AIDS patients.

TL;DR: Checkerboard analysis revealed both chemotactic and chemokinetic components to this response to TGF beta, which may recruit monocytes from the circulation, and subsequently activate them to express angiogenic activities such as TNF alpha, thus playing an important role in wound repair, inflammation and tumor growth.

TL;DR: The amino acid sequence of the KEX2 gene product deduced from nucleotide sequencing indicates that the Kex2-encoded protein may function as a membrane-bound, subtilisin-like serine protease.

TL;DR: Results indicate that the C-terminal Trp of ET is especially important for the potent and extremely long-lasting vasoconstrictor activity characteristic to ET.

TL;DR: It is concluded that both the dioxin and glucocorticoid receptor can form heteromeric complexes which share a common non-ligand-binding component.

TL;DR: The observation that basic FGF mRNA is below the limits of detection in adrenal, spleen, heart, lung, kidney, liver, stomach, small intestine, large intestine, testis, and ovary support the notion that the that the high levels of the protein found in these tissues is due to storage of the mitogen in the extracellular matrix and not continuous gene expression.