Showing papers in "Biochemical and Biophysical Research Communications in 1992"

TL;DR: The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF, flt, in a manner reminiscent of the similarities between the alpha and beta forms of the PDGF receptors.

TL;DR: It is demonstrated that like bFGF, VEGF induces an angiogenic response via a direct effect on endothelial cells, and that by acting in concert, these two cytokines have a potent synergistic effect on the induction of angiogenesis in vitro.

TL;DR: The molecular weights of the released molecules have been determined by immunoprecipitation and are consistent with the generation of soluble forms by cleavage at a site close to the point of membrane insertion.

TL;DR: It is shown here that ABP and an internal fragment encompassing residues 25 to 35 (beta 25-35) are cytotoxic to a clone of PC12 cells at concentrations above 1 x 10(-9)M and to several other cell lines at higher concentrations.

TL;DR: 6 additional isoforms of the NMDA receptor generated via alternative splicing by molecular analysis of cDNA clones isolated from a rat forebrain cDNA library are reported, indicating that theNMDA receptor consists of heterogeneous molecules that differ in the extracellular sequence of the amino- and carboxyl-terminal regions.

TL;DR: Using a subtraction cloning approach, a set of cDNA clones from mouse neural precursor cells whose respective mRNA levels are down-regulated during the development of mouse brain are isolated and indicate that these genes may play important roles during the embryonic development and differentiation of central nervous system.

TL;DR: Saturation binding analysis indicates that the human BK-2 receptor expressed in COS-7 cells binds BK with a KD of 0.13 nM, and Pharmacological characterization of the expressed BK receptor is consistent with the cDNA encoding a receptor of the Bk-2 subtype.

TL;DR: The pressor effect of SRTX S6C was not dependent on central stimulation of ETB receptors and was not mediated by catecholamines from the adrenal medulla, prostanoids or ET-1, indicating that the in vivo vasoconstrictor effect is most likely indirect.

TL;DR: It is demonstrated that IL-10, a product of Th2 (helper T lymphocyte 2) cells, can antagonise the function of IFN-gamma, a products of Th1 cells, by modulating the mechanism of synthesis of nitric oxide in the macrophages.

TL;DR: Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids.

TL;DR: Three days after unilateral hypoxic-ischemic injury in infant rats insulin-like growth factor 1 (IGF-1) production by astrocytes was enhanced in the injured region and it was suggested that endogenous IGF-1 is neurotrophic and that centrally administered IGF- 1 may have therapeutic potential for brain injury.

TL;DR: The isolation of a cDNA representing the human pituitary GnRH receptor encodes a protein with a transmembrane topology similar with that of other G protein-coupled, 7-trans Membrane receptors.

TL;DR: It is suggested that amplification of the c-met gene might participate in carcinogenesis and progression of stomach cancer, especially scirrhous type stomach carcinoma.

TL;DR: A mitochondrial DNA mutation at nucleotide position 14,484 was found in 14 independent probands with Leber hereditary optic neuropathy and in 0/250 controls and the 13,708 secondary mutation appears to play a central role in this process.

TL;DR: The cloning, sequencing, and expression of a previously unsuspected second form of the type 1 receptor (AT1B) in the rat which exhibits high similarity with the AT1A receptor relative to amino acid sequence, binding of angiotensin II analogs, and utilization of Ca+2 as its intracellular second messenger is reported.

TL;DR: It is demonstrated that blockade of neurotransmission by tetanus toxin in isolated nerve terminals is associated with a selective proteolysis of synaptobrevin, an integral membrane protein of synaptic vesicles, and data suggest that cleavage ofsynaptob revin is the molecular mechanism of tetanus toxins action.

TL;DR: Results suggest that RGD sites in type I collagen may be masked and that they become exposed upon denaturation of the molecule, andounding of extracellular matrix may, thus, expose RGD Sites in collagens that facilitate the interaction of cells with damaged extrace cellular matrix through RGD binding integrins.

TL;DR: The existence of two structurally distinct forms of the angiotensin receptor AT-1 in the mouse is reported and it is speculated that these isoforms may differ in their regulation, signal transduction, or desensitization mechanisms.

TL;DR: The results indicate that accumulation of somatically acquired oxygen damage together with age-associated mutations in mtDNA which lead to bioenergetic deficiency and the heart muscle weakness are inevitable in human life.

TL;DR: It is shown that H2O2 inhibits a protein tyrosine phosphatase activity, but not a p-nitro phenyl phosphate hydrolysing activity, in cytosolic extracts of intact rat hepatoma cells, and that vanadate markedly potentiates this effect although it has only trivial effects of its own.

TL;DR: Evidence is provided in favour of an association of HT with a polymorphism at the ACE locus (17q23), so implicating this locus, and possibly a genetic variant of ACE itself, in human essential hypertension.

TL;DR: Results obtained in normal epithelial cells indicate that cyclin A is involved in S phase and thus should not be only considered as a mitotic cyclin.

TL;DR: The data suggest that vascular smooth muscle cells express ETA and ETB receptors, and Stimulation of either receptor subtype can result in force development.

TL;DR: Observations indicate clearly that NO formed during the NO synthase reaction or added to the enzyme reaction mixture causes a reversible inhibition of No synthase activity, indicating that NO may function as a negative feedback modulator of its own synthesis.

TL;DR: Two unique antimicrobial peptides named brevinin-1 and -2 were isolated from the skin of the frog, Rana brevipoda porsa, indicating the difference of the two peptides in the antimicrobial selectively on Gram-positive and Gram-negative bacteria.

TL;DR: The signalling mechanism and cellular targets of the AT2 receptor are still unknown as discussed by the authors, but it has been shown that angiotensin II (Ang II) inhibits basal and atrial natriuretic peptide stimulated particulate guanylate cyclase (pGC) activity through AT2 receptors in rat adrenal glomerulosa and PC12W cells.

TL;DR: Human monomorphic and polymorphic arylamine acetyltransferases expressed in monkey kidney COS-1 cells were used to study the N- and O-acetylation of a number of carcinogenic amines and their N-hydroxy metabolites and suggest that rapid acetylator individuals will be predisposed to their genotoxicity.

TL;DR: IRL 1620 (10(-9)-10(-7) M) induced contractions of the guinea pig trachea with a comparable potency to those of ET-1 or ET-3, suggesting that IRL 1520 is a potent ETB receptor agonist.

TL;DR: Results indicate that α-lipoic acid may be effective in AIDS therapeutics, and reports that antioxidants which eliminate ROS should block the activation of NF-κB and subsequently HIV transcription, and thus antioxidants can be used as therapeutic agents for AIDS.

TL;DR: The cDNA was used to isolate the human CCKB receptor homologue from brain and stomach which encodes a 447 amino acid protein with 90% identity to both rat CCKB and canine gastrin receptors.