Showing papers in "Biochemical and Biophysical Research Communications in 1993"

TL;DR: Occurrence of adrenomedullin indicates the possible existence of a novel system for circulation control and suggests that adrenomed Mullin is a new hormone participating in blood pressure control.

TL;DR: A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm.

TL;DR: By RNA blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues including adrenal medulla, ventricle, lung and kidney as well as pheochromocytoma.

TL;DR: An algorithm for identifying proteins at the sub-microgram level without sequence determination by chemical degradation is developed, providing a rapid and sensitive link between genomic sequences and 2D gel electrophoresis mapping of cellular proteins.

TL;DR: The putative copper and ATP-binding domains of the human Menkes disease gene were used as probes to screen a human liver cDNA library at reduced stringency and suggest that this cDNA is a candidate gene for Wilson disease and that the protein encoded at this locus is a member of the P-type ATPase family.

TL;DR: Cloned a rat cDNA encoding a functional serotonin receptor (5-HT6), its coding sequence corresponds to a glycoprotein of 436 amino acids displaying significant homology with other cloned monoaminergic receptors, e.g., various serotonin receptors.

TL;DR: Porphyrinic sensors were used for the in situ monitoring of nitric oxide release and diffusion in the endothelial cell, as well as its subsequent diffusion from the endothelium through the muscle cells found in the rabbit aorta.

TL;DR: It is demonstrated that human retinal pigment epithelial cells can synthesize the secreted angiogenic peptide vascular permeability factor/vascular endothelial growth factor in vitro and in situ and may be important in the pathogenesis of ocular neovascularization.

TL;DR: Findings and kinetics studies suggest the involvement of NO synthase in apoptosis induction, which led to a L-arginine-dependent M phi cell death by apoptosis in parallel to nitrite and citrulline production.

TL;DR: A novel NO-dependent mechanism that upregulates ciliary motility in response to stimulation is demonstrated that modulates airway ciliary beating and was completely reversed by L-arginine or SNP but not by D- arginine.

TL;DR: RNA blot analysis showed that rat adrenomedullin mRNA was expressed in adrenal glands, lung, kidney, heart, spleen, duodenum and submandibular glands, and elicits a potent and long-lasting hypotensive activity in anesthetized rats.

TL;DR: The results suggest that the MC-2 DNA codes for a novel melanocortin receptor, a characteristic of G-protein coupled receptors, which is expressed in brain tissue but not in the melanoma cells.

TL;DR: It is reported that exposure of cultured human liver FSC to the pro-oxidant system ascorbate/iron results in an early induction of lipid peroxidation, as monitored in terms of MDA and fluorescent aldehyde/protein adducts production, and indicates that oxidative reactions can directly influence procollagen I gene expression and synthesis in FSC, thus contributing to the development of liver fibrosis.

TL;DR: Findings show that the NOS is inducible and could regulate the proliferation of VSMC and the amino acid sequence deduced from the cloned NOS cDNA was distinct from that of the previously reported constitutive types of NOSs, while highly similar to that of macrophage NOS.

TL;DR: Rate constants for the reactions of several organic peroxyl radicals with nitric oxide in aqueous solution are measured and it is found that they are all fast, with k = 1-3 x 10(9) L mol-1 s-1.

TL;DR: It is concluded that hypoxic conditions may promote cardiac capillary cell growth by inducing VEGF expression.

TL;DR: The results suggest that adrenomedullin induces nonadrenergic and noncholinergic vasodilation in which CGRP receptors may be involved.

TL;DR: Human articular chondrocytes express an inducible NO synthase from the same family as the rabbit chondROcyte and rodent macrophage enzymes, which appears to vary in terms of in vitro Ca(2+)-dependence and sensitivity to glucocorticoids.

TL;DR: Using bacterial CpG methylase to evaluate the effect of folate deficiency on methylation of DNA from livers of weanling rats fed an amino acid-defined folate deficient diet, liver DNA from deficient animals was undermethylated compared with DNA from pair-fed control animals.

TL;DR: The results indicate that nattokinase is a subtilisin-like serine protease, a strong fibrinolytic enzyme that digested not only fibrin but also several synthetic substrates.

TL;DR: The purification procedure involved three sequential chromatography steps on hydroxylapatite, immobilized Cu++ and immobilized anti-CD38 monoclonal antibody resins, which yielded a single 46 kDa protein displaying all three enzymatic activities.

TL;DR: Highly degenerate PCR primers were designed based on the amino acid sequence of the zinc finger motifs of known nuclear receptor members to aid in the identification of additional members of the nuclear receptor superfamily and measures relative changes in the expression of nuclear receptors between two different biological states.

TL;DR: RNA blot and in situ hybridization analyses show that the delta 2 sub unit mRNA is localized in cerebellar Purkinje cells, and immunoblot and immunohistochemical analyses suggest that the Delta 2 subunit protein is actually expressed in vivo in Purkinja neurons.

TL;DR: The results indicate that LPS treatment in vivo induces the expression of an iNOS mRNA via a dexamethasone-sensitive mechanism, and thus provide direct molecular evidence for the involvement of NO in septic shock.

TL;DR: It is suggested that apo E, as well as other related proteins may bind not only amyloid Aβ but also sAβ, a normal soluble peptide found in the cerebrospinal fluid and other biological fluids, which may be critical in amyloids formation.

TL;DR: A DNA sequence that confers a response to a class of rodent hepatocarcinogens termed peroxisome proliferators has been identified 2947bp upstream of the rat peroxISomal bifunctional enzyme gene and it is shown that two members of the steroid hormone receptor family co-operate to bind specifically to this sequence.

TL;DR: Results suggest that the tyrosine phosphorylation of IRS-1 and activation of PI3-kinase may be involved in the signaling pathway of the insulin-stimulated GLUT4 translocation.

TL;DR: The sequence and functional expression of a fourth and a fifth human somatostatin receptor subtype, termed hSSTR4 and hSstr5, respectively are reported, suggesting that diverse actions of somatstatin are mediated by at least five somatOSTatin receptorSubtypes with potentially different function.

TL;DR: Findings suggest iNOS activity in macrophages treated with LPS + IFN gamma requires NF-kappa B activation, and Pyrrolidine dithiocarbamate added to cultured macrophage stimulated with lipopolysaccharide and interferon-gamma suggests this requirement.

TL;DR: It is suggested that under specific inflammatory conditions the generation of NO in vascular tissue switches from ec-cNOS in the endothelium to iN OS in the smooth muscle.