Showing papers in "Biochemical and Biophysical Research Communications in 1996"

TL;DR: The apM1 gene encodes a 244 amino acid open reading frame containing a putative signal sequence and G-X-Y repeats (66 amino acids) followed by a cluster of aromatic residues near the C terminus having high local similarity with collagens X and VIII and complement factor C1q.

TL;DR: A screening of hemopoietic cell lines showed that the Iba1 protein was clearly expressed in monoblastic cell lines but only very weakly expressed in myeloid cell lines, suggested to act as an adapter molecule, mediating calcium signals that may function in a monocytic lineage including microglia.

TL;DR: Human sFLT-1 is shown to form a VEGF-stabilized complex with the extracellular domain of KDR in vitro, suggesting that not only full-length receptors are capable of forming ligand-induced heterodimeric complexes.

TL;DR: The nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland, is reported, which contains a protein of 643 amino acids with 84% identity to the rat NIS (rNIS).

TL;DR: Diallelic polymorphism (Ala-9Val) in the MTS of human MnSOD in a Japanese population is identified and it is suggested that this mutation may reflect functional polymorphism of mitochondrial transport of humanmnSOD.

TL;DR: It is hypothesized that amyloid formation could be inhibited by peptides homologous to A beta with a similar degree of hydrophobicity, but with a very low propensity to adopt a beta-sheets conformation by incorporating proline residues (anti-beta-sheet peptides or beta-sheet inhibitors).

TL;DR: It is shown that the EGFP expression vector delivered into mammalian cells gives rise to bright fluorescence that is readily detectable following a 16-24 hr transfection interval, and visual detection of transfected cells with EGFP appears to be more sensitive than equivalent measurements with beta-galactosidase catalyzed conversion of the X-gal substrate.

TL;DR: It is suggested p38 gamma may have unique functions when compared with other members of the p38 group due to its restricted tissue expression and apparent substrate preferences.

TL;DR: A cDNA clone from a human placental choriocarcinoma cell (JAR) cDNA library which codes for a functional type 1 sigma receptor is isolated, and the inhibitor specificity of the cloned hSigmaR1 indicates that it is the type 1Sigma receptor.

TL;DR: The ubiquitous in vivo expression of Hif-1 alpha and HIF-1 beta (ARNT) mRNA is consistent with the proposed role of H IF-1 in coordinating adaptive transcriptional responses to hypoxia.

TL;DR: The results indicate that the antidiabetic effects of thiazolidinediones in humans are likely to be mediated via binding to and transactivation of PPARγ1 and γ2.

TL;DR: Findings suggest that OCT2 is responsible for the transport of cationic drugs in the kidney, and that this transcript was not detected in the brain, heart, lung, liver, small intestine or spleen.

TL;DR: Results indicate that BMPs induce the differentiation of bone marrow stromal cells into osteoblasts, however, the effects differ among the B MPs and among the types of cell exposed to these proteins.

TL;DR: Findings suggest the possibility that the binding of leptin to its receptor in beta-cells may modulate insulin expression in a negative feedback loop, and thereby may have an anti-obesity effect.

TL;DR: The gene encoding of the 65 kDa isoform of the gamma-aminobutyric acid (GABA)-synthesizing enzyme, glutamic acid decarboxylase (GAD), GAD65, was targeted in mice by homologous recombination and there was no difference in GABA content in the brains of G AD65 +/+, +/-, and -/- mice.

TL;DR: Elevation of the L-arginine/ADMA ratio may at least partly explain the restored NO formation by exogenous L- arginine in hypercholesterolemia.

TL;DR: The deduced amino acid sequence contains a putative signal sequence, two hydrophobic regions at the carboxy terminus, two potential Asn-linked glycosylation sites, and four regions in the amino-terminal half that are highly conserved in known apyrases and related enzymes.

TL;DR: A sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats, finding that leptin levels were unchanged in fa/fa rats exposed to the same physiological stimuli.

TL;DR: There is a broad, but tissue specific distribution of opioid receptor expression in the periphery of the rat, suggesting that the endogenous opioid peptides play an endocrine, paracrine, or autocrine role in the regulation of physiology at a peripheral as well as central level.

TL;DR: In this article, the P2Y6 receptor was identified as the human ortholog (88% amino acid identity) of the rat receptor recently cloned by Chang et al. (J. Biol. Chem. 270, 26152-26158, 1995).

TL;DR: In this paper, the transmembrane (TM) domains of the D2 receptor were mimicked by peptides derived from the TM domains, and the peptides were unable to dissociate dopamine D1 and serotonin 5-HT1B receptor dimers.

TL;DR: It is concluded that osteocytes are more sensitive to fluid shear stress than immature bone cells, and that the actin-cytoskeleton is involved in the response to fluid flow.

TL;DR: A potent and structurally novel antimicrobial peptide was isolated and characterized from the stomach tissue of Bufo bufo gargarizans, an Asian toad, and was found to be more potent than magainin 2.

TL;DR: It is reported that EDHF-mediated relaxations in the rat mesenteric arterial bed are blocked by a highly selective cannabinoid receptor antagonist, SR141716A, consistent with EDHF being a cannabinoid-like substance, and anandamide is a potent vasorelaxant in the mesentery, acting via a hyperpolarizing mechanism.

TL;DR: MRNAs encoding the neural genes Wnt-1, MASH1, the light and medium isoforms of neurofilaments, and the neurotransmitter-synthesizing enzyme glutamic acid decarboxylase are all strongly upregulated by retinoic acid treatment; expression of these genes occurs in a temporal pattern resembling that in the developing brain.

TL;DR: R reverse transcription-polymerase chain reaction analysis revealed that Ob-R isoform messenger RNAs (mRNAs) are expressed in a wide variety of rat tissues in tissue-specific manners and were present in the brain from Zucker fatty (fa/fa) rats at comparable amounts to those in their lean littermates.

TL;DR: In this article, the immunocytochemical localization of nitric oxide synthase in mitochondria isolated from heart, skeletal muscle, and kidney is demonstrated using a monoclonal antibody directed against the endothelial form of nitricside synthase.

TL;DR: Receptor binding analysis of several structurally different dopamine D3 ligands showed similar pharmacological properties for all compounds tested except for dopamine and the D3-selective ligand GR99841, but a significantly higher dopamine binding affinity for the Ser9Gly homozygote was detected.

TL;DR: Observations show that PepT1 is specific to the brush border of the differentiated absorptive epithelial cells and suggest that H+-coupled uptake of small peptides and peptide-like drugs occurs at the apical membrane of these cells in the small intestine.

TL;DR: The results suggest that p21 protein expression could be increased by transcriptional mechanisms as well as inhibition of proteolysis by LC, suggesting transcriptional induction of p21.