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Showing papers in "Biochemical and Biophysical Research Communications in 2000"


Journal ArticleDOI
TL;DR: Molecular phylogenetic analysis of 60 sirtuin conserved core domain sequences from a diverse array of organisms shows that eukaryotic Sir2-like proteins group into four main branches designated here as classes I-IV.

1,354 citations


Journal ArticleDOI
TL;DR: While gh Relin activates growth-hormone secretagogue (GHS) receptor-expressing cells, the nonmodified des-n-octanyl form of ghrelin, designated as des-acyl ghrel in, does not.

928 citations


Journal ArticleDOI
TL;DR: It is suggested that the stimulatory effect of the ionic products of Bioglass 45S5 dissolution on osteoblast proliferation may be mediated by IGF-II.

904 citations


Journal ArticleDOI
TL;DR: Intravenous administrations of rat ghrelin at 0.8 to 20 microgram/kg dose-dependently increased not only gastric acid secretion measured by a lumen-perfused method, but also gastric motility measured byA miniature balloon method suggest that gh Relin may play a physiological role in the vagal control of gastric function in rats.

882 citations


Journal ArticleDOI
TL;DR: Polyamines were found to modulate protein synthesis at several different levels including stimulation of special kinds of protein synthesis, stimulation of the assembly of 30 S ribosomal subunits and stimulation of Ile-tRNA formation.

826 citations


Journal ArticleDOI
TL;DR: It is shown, in a bird, that the hypothalamus also contains a novel peptide which inhibits gonadotropin release, the first hypothalamic peptide inhibiting gonadotropic hormone reported in a vertebrate.

760 citations


Journal ArticleDOI
TL;DR: Three novel transporters structurally belonging to the organic anion transporting polypeptide (OATP) family in humans are identified and OATP-C proved to be identical with the recently reported LST1/OatP-2.

621 citations


Journal ArticleDOI
TL;DR: Results suggest that FGF10 acts as a major ligand for FGFR2b in mouse multi-organ development, which would indicate a role for FGF12 in wide-range organogenesis.

608 citations


Journal ArticleDOI
TL;DR: In this article, the authors quantify the viscoelastic properties of mechanically and chemically isolated nuclei of articular chondrocytes using micropipet aspiration in conjunction with a theoretical visco-elastic model.

548 citations


Journal ArticleDOI
TL;DR: In this article, the 23rd documented FGF, FGF-23 has a hydrophobic amino terminus (approximately 24 amino acids), which is a typical signal sequence, and was found to be preferentially expressed in the ventrolateral thalamic nucleus.

538 citations


Journal ArticleDOI
TL;DR: The present study indicates the usefulness of LRP Western blot analysis, which shows that the modulation of osteoclastogenesis by humoral factors is achieved, in part, by regulation of the expression of RANKL and OPG in osteoblast/stromal lineage cells.

Journal ArticleDOI
TL;DR: It is demonstrated that phycocyanin is a potent peroxyl radical scavenger with an IC(50) of 5.0 microM and the rate constant ratios obtained for phydynamic acid and uric acid were 1.54 and 3.5, respectively, which clearly suggest that the covalently linked chromophore, phyCocyanobilin, is involved in the antioxidant and radical scavenging activity of phy cokyanin.

Journal ArticleDOI
TL;DR: The characterization of neural progenitors isolated from the adult mammalian eye, located in the pigmented ciliary bodies, proliferate in the presence of FGF2 and express the neuroectodermal marker nestin, finds that these quiescent cells give rise to neural spheres and are multipotential.

Journal ArticleDOI
TL;DR: Endogenous ABC1 gene expression in RAW cells and apolipoprotein A-I mediated cholesterol efflux were also upregulated by both receptor ligands, raising the possibility that ligands that activate the LXR-RXR heterodimer may be useful for the therapeutic modulation of the ABC1 pathway.

Journal ArticleDOI
TL;DR: Adding these compounds to commonly used cell culture media leads to generation of substantial amounts of hydrogen peroxide (H(2)O(2), which could account for some or all of the reported effects of phenolic compounds on cells in culture.

Journal ArticleDOI
TL;DR: Results indicate that NO production is modulated by the uptake, recycling, and degradation of arginine, and this may guarantee the efficient production of NO.

Journal ArticleDOI
TL;DR: Evidence is presented that patients with the rare disorder X-linked cardioskeletal myopathy and neutropenia (Barth syndrome, MIM 302060) have a primary defect in CL and PG remodeling, and an essential factor in this important cellular process is identified for the first time.

Journal ArticleDOI
TL;DR: Exercise at a higher intensity for only 20 min leads to increases in AMPK alpha2 activity but not alpha1 activity, suggesting that the alpha2-containing AMPK complex, rather than alpha1, may be involved in the metabolic responses to exercise in human skeletal muscle.

Journal ArticleDOI
TL;DR: Observations indicate that the clone is a distinct histamine receptor from HH3R, and thus is named HH4R, which was cloned from human leukocyte cDNA.

Journal ArticleDOI
TL;DR: It is demonstrated that PPAR-gamma was expressed in human lung cancer cell lines by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis, and that PPar-Gamma agonist may be useful therapeutic agents in the treatment of human lung cancers.

Journal ArticleDOI
TL;DR: Physiological concentrations of H(2)S selectively enhance NMDA receptor-mediated currents and facilitate the induction of hippocampal long-term potentiation (LTP) in brain cells, neuronal and glial cell lines, and Xenopus oocytes are shown.

Journal ArticleDOI
TL;DR: Ghrelin neuron is present in the arcuate nucleus of rat hypothalamus, but its central effect on growth hormone (GH) release has yet to be clarified, and the plasma GH concentration and GH mRNA level in the pituitary in response to central administration of ghrelin is determined.

Journal ArticleDOI
TL;DR: It is found that ERα but not ERβ-inactivated male mice develop obesity after sexual maturation, and the estrogen receptor (ER) specificity of such effects is dissected.

Journal ArticleDOI
TL;DR: The purification of full-length recombinant ADAM 12-S is described and it is demonstrated that it cleaves insulin-like growth factor binding protein-3 (IGFBP-3) and this result supports a role for ADAM 11-S in the degradation of IGF BP-3 in the blood of pregnant women.

Journal ArticleDOI
TL;DR: AQP9 proteins is strongly expressed in rat liver, testes, epididymis, spleen, and brain and preabsorption with the immunizing peptides eliminated the labeling.

Journal ArticleDOI
TL;DR: Yeast ER chaperone Kar2p/BiP, a member of the HSP70 family found in the ER, directly regulates the unfolded protein response by the interaction with Ire1p, similar to the regulation of mammalian cytosolic chaper one Hsp70 via its interaction with heat shock factor 1.

Journal ArticleDOI
TL;DR: Based on the physicochemical distance between amino acids, a set of sequence-order-coupling numbers was introduced to reflect the sequence order effect, or in a rigorous term, the quasi-sequence-order effect and a remarkable improvement was observed in the prediction quality using the augmented covariant discriminant algorithm.

Journal ArticleDOI
TL;DR: Results showed that pioglitazone significantly increased the apoA-I secretion from the human hepatoma cell line HepG2 and binding assay indicated that the transactivation of hPPARgamma1 or hPParalpha by piog Litazone is due to direct binding of piog litazone to each subtype.

Journal ArticleDOI
TL;DR: Results suggest that statin-mediated activation of BMP-2 promoter was completely inhibited by the downstream metabolite of HMG-CoA reductase, mevalonate, indicating that the activation was a result of the inhibition of the enzyme.

Journal ArticleDOI
TL;DR: The present study points out that the hepatoprotective, anti-inflammatory, and anti-arthritic properties of phycocyanin reported in the literature may be due, in part, to its selective COX-2 inhibitory property, although its ability to efficiently scavenge free radicals and effectively inhibit lipid peroxidation may also be involved.