scispace - formally typeset
Search or ask a question

Showing papers in "Biochemical and Biophysical Research Communications in 2002"


Journal ArticleDOI
TL;DR: Gel mobility shift assay using mutant DREB proteins showed that the two amino acids, valine and glutamic acid conserved in the ERF/AP2 domains, especially valine, have important roles in DNA-binding specificity.

1,589 citations


Journal ArticleDOI
TL;DR: A novel category of bile acid receptor, a membrane-type G protein-coupled receptor (GPCR), BG37 was detected in various specific tissues, suggesting its physiological role, although it remains to be further characterized.

826 citations


Journal ArticleDOI
TL;DR: Following neuronal induction of murine and human ADAS cells, Western blot analysis confirmed GFAP, nestin, and NeuN protein expression and Pretreatment with EGF and basic FGF augmented the neuronal differentiation of huADAS cells.

811 citations


Journal ArticleDOI
TL;DR: Observations suggest that CBS activity is reduced in AD brains and the decrease in H2S may be involved in some aspects of the cognitive decline in AD.

734 citations


Journal ArticleDOI
TL;DR: The results suggest that adiponectin gene expression is reversibly downregulated by insulin, TNFalpha, and dexamethasone, and support the concept of adip onectin being an important selectively controlled modulator of insulin sensitivity.

663 citations


Journal ArticleDOI
TL;DR: In this article, the authors examined the chondrogenic potential of adipose tissue-derived stromal cells and found that these cells abundantly synthesized cartilage matrix molecules including collagen type II, VI, and chondroitin 4-sulfate.

620 citations


Journal ArticleDOI
TL;DR: The Surface Enhanced Laser Desorption/ionization Time-of-flight mass spectrometry (SELDI-TOF MS) is a novel approach to biomarker discovery that combines two powerful techniques: chromatography and mass spectrametry.

555 citations


Journal ArticleDOI
TL;DR: Using Cytofectin GSV to deliver both inhibitors, the siRNAs appear to be quantitatively more efficient and its effect is lasting for a longer time in cell culture and in mice, an activity of siRNA but not of antisense oligonucleotides is observed.

551 citations


Journal ArticleDOI
TL;DR: The concepts, challenges, and limitations in bone tissue engineering are outlined and the potential that could improve the quality of life for many as a result of interdisciplinary collaboration is outlined.

549 citations


Journal ArticleDOI
TL;DR: The mAb against TLR3 reported herein may serve as a regulator for virus-mediated immune response via an alternative pathway involving the dsRNA-TLR3 recognition which might occur on host cells.

481 citations


Journal ArticleDOI
TL;DR: It is demonstrated that a fullerene derivative could cross the external cellular membrane and it localises preferentially to the mitochondria, and it is proposed that this finding supports the potential use of fullerenes as drug delivery agents as their structure mimics that of clathrin known to mediate endocytosis.

Journal ArticleDOI
TL;DR: To the authors' knowledge, this is the first evidence that creatine has the potential to act as a direct antioxidant against aqueous radical and reactive species ions.

Journal ArticleDOI
TL;DR: A new quantitative method is proposed which represents a simple but accurate quantitative method which is capable of measuring cycle-by-cycle PCR amplification efficiencies and demonstrates that these change dynamically.

Journal ArticleDOI
TL;DR: The results may suggest that an acute bout of low-intensity prolonged swimming exercise directly enhances the PGC-1 mRNA expression in the activated muscle during exercise, possibly through, at least in part, an AMPK-related mechanism.

Journal ArticleDOI
TL;DR: It is shown for the first time that ghrelin increases memory retention, suggesting that the peptide may influence processes in the hippocampus, as well as inducing anxiogenesis in rats.

Journal ArticleDOI
TL;DR: It is concluded that functional caspase 12 is lost in humans and that it can therefore not play a role in Alzheimer's disease.

Journal ArticleDOI
TL;DR: The results indicate that many side-chains can be accommodated in the P1' position of a TEV protease recognition site with little impact on the efficiency of processing.

Journal ArticleDOI
TL;DR: This work provides further evidence that ChoK dysregulation is a frequent event found in a variety of human tumors such as lung, colorectal, and prostate tumors, and suggests thatChoK could be used as a tumor marker.

Journal ArticleDOI
TL;DR: It is shown that there are gender differences in serum BPA concentrations, possibly due to differences in the androgen-related metabolism of BPA, which is a widely used endocrine disruptor.

Journal ArticleDOI
TL;DR: The data suggest that bisphosphonates modulate OPG production by normal human osteoblasts, which may contribute to the inhibition of osteoclastic bone resorption.

Journal ArticleDOI
TL;DR: The results indicate that the recombinant mPGE synthase is identical to the enzyme purified from the microsomal fraction of bovine heart, and is a novel type of mPGe synthase based on the primary structure, a broad specificity of thiol requirement, and tissue distribution.

Journal ArticleDOI
TL;DR: The results suggest that TBBPA and TCBPA act as thyroid hormone agonists, as well as estrogens.

Journal ArticleDOI
TL;DR: Increased muscle development in Myostatin knockout mice is associated with reduced adipogenesis and consequently, decreased leptin secretion, and levels in adipose tissue were significantly lower in KO mice compared to WT mice.

Journal ArticleDOI
TL;DR: The high resolution structure of hemalbumin was determined by single crystal X-ray diffraction and revealed the protoporphyrin IX bound to a single site within a hydrophobic cavity in subdomain IB, one of the principal binding sites for long chain fatty acid.

Journal ArticleDOI
TL;DR: A novel protein probe, molecular aptamer beacon (MAB), for real time protein recognition and quantitative analysis that combines the signal transduction mechanism of molecular beacons and the molecular recognition specificity of aptamers.

Journal ArticleDOI
TL;DR: The physiological significance of histaminergic neurons in the orexin-induced arousal responses is shown, which strongly suggest that activation of histaminationergic neurons by orexins might be important for modulation of the arousal.

Journal ArticleDOI
TL;DR: In vitro, apelin-13 stimulated the release of CRH and AVP from hypothalamic explants, but had no effect on NPY release, suggesting that apelin may play an important role in the hypothalamic regulation of water intake and endocrine axes.

Journal ArticleDOI
TL;DR: In this article, the authors examined whether H(2)O(2)-derived hyperpolarizing factor (EDHF) is a primary EDHF in human mesenteric arteries with some contribution of gap junctions.

Journal ArticleDOI
TL;DR: It is shown that MCF-7 cells express leptin receptors and respond to human recombinant leptin by STAT3 and p42/p44 MAPkinase activations and by increased proliferation, suggesting that leptin could act in vivo as a paracrine/endocrine growth factor towards mammary epithelial cells thus contributing to explain why obesity is a risk factor of developing breast cancers.

Journal ArticleDOI
TL;DR: A biophysical model for the action of oscillating electric fields on cells is extended now to include oscillating magnetic fields as well, extended to include the most active biological conditions, and also to explain why pulsed electromagnetic fields can be more active biologically than continuous ones.