Showing papers in "Biochemical and Biophysical Research Communications in 2004"

TL;DR: In this paper, quantitative real-time PCR was used to determine the mRNA transcription profiles of 13 putative reference genes, comparing their transcription in 16 different tissues and in CCRF-HSB-2 cells stimulated with 12-Otetradecanoylphorbol-13-acetate and ionomycin.

TL;DR: This work analyzed the structural variations, which are the basis of functional diversification, as well as the genomic organization of the S100 family in human and compared it with the S 100 repertoires in mouse and rat, and identified evolutionary related subgroups of S100 proteins within the three species.

TL;DR: The presented data support the idea that adiponectin might be of critical relevance for cytokine regulation in obesity and fatty liver diseases affecting primarily macrophage functions and could represent a fundamental link between over-nutrition and an impaired inflammatory immune response.

TL;DR: It is concluded that there is no evidence that cancer cells are inherently glycolytic, but that some tumours might indeed be glyCOlytic in vivo as a result of their hypoxic environment.

TL;DR: Chloroquine, an old antimalarial drug, may be considered for immediate use in the prevention and treatment of SARS-CoV infections.

TL;DR: Protein-tyrosine phosphatases such as PTPalpha displace phosphotyrosine 527 from the Src SH2 domain and mediate its dephosphorylation leading to Src kinase activation, which results in inhibition from phosphorylation by C-terminal SRC kinase.

TL;DR: It is shown that subcutaneous administration of metastin increased the plasma levels of gonadotropins and induced ovulation in prepubertal female rats that had been pretreated with pregnant mare serum gonadotropic hormone to induce follicle maturation and that s.c. administered metastin induces the release of Gonadotropin via activation of the hypothalamic GnRH neurons.

TL;DR: New insights are revealed into the molecular basis of membrane dynamics and the regulation of autophagy, which had remained cryptic for a long time.

TL;DR: It is shown that ERK and ERK-dependent NF-kappaB activation is required for oxidative stress-induced inhibition of osteoblastic differentiation in rabbit BMSC and calvarial osteoblasts.

TL;DR: Testing the hypothesis that the anti-inflammatory action of adiponectin includes suppression of IL6 and an induction of IL10 in activated human macrophages indicates that this action is mediated in part by suppression of NFkappaB signaling and ERK1/2 activity.

TL;DR: The results suggest that the balance in the concentrations of adipocytokines such as resistin and adiponectin determines the inflammation status of vasculature, and in turn the progress of atherosclerosis.

TL;DR: An increasing body of literature suggests a causal link between calcineurin signaling and the cardiac hypertrophic response and heart failure through the use of pharmacologic inhibitors (cyclosporine A and FK506) and genetic approaches.

TL;DR: The ErbB receptors are targets for anticancer drugs and two strategies for blocking the action of these proteins include antibodies directed against the ectodomain and drugs that inhibit protein-tyrosine kinase activity.

TL;DR: This study has demonstrated that cartilage tissue can be created from bone marrow mesenchymal stem cells and shown particular patterns of in vitro chondrogenesis induction on human bone marrow MSCs (hBMSCs) by cycling the growth factors.

TL;DR: The results reinforce the notion that the excessive action of FGF-23 plays a causative role in the development of several hypophosphatemic rickets/osteomalacia.

TL;DR: A selection algorithm based on these findings strongly differentiated between the two functional groups of siRNA in both data sets and proved highly effective when used to design siRNA targeting new endogenous human genes.

TL;DR: The results suggest that the endogenous CSE/H(2)S pathway contributes to the pathogenesis of isoproterenol-induced myocardial injury, at least by its direct scavenging of oxygen-free radicals and reducing the accumulation of lipid peroxidations.

TL;DR: These findings attest to the feasibility of completely destroying advanced, highly glycolytic cancers.

TL;DR: The data suggest that oxidative stress contributes to worsening inflammation via reduction of HDAC2 activity throughHDAC2 nitration, and this novel mechanism of inflammation may be important in increasing the severity and chronicity of inflammatory diseases.

TL;DR: It is shown that a recombinant fusion protein (designated RBD-Fc) containing 193-amino acid RBD and a human IgG1 Fc fragment can inducehighly potent antibody responses in the immunized rabbits and suggests that RBD can induce highly potent neutralizing antibody responses and has potential to be developed as an effective and safe subunit vaccine for prevention of SARS.

TL;DR: It is demonstrated that Astragalus polysaccharide fractions activates mouse B cells and macrophages, but not T cells, in terms of proliferation or cytokine production, indicating that APS activates B cells via membrane Ig in a TLR4-independent manner.

TL;DR: It is suggested that H2S could be endogenously produced by heart tissues, as a physiological cardiac function regulator, mediated by KATP channel pathway.

TL;DR: The structural characterization of an acidic isoform of DJ-1 using a proteomic approach with nanospray interface liquid chromatography-electrospray ionization/linear ion trap mass spectrometer suggests thatDJ-1 function is regulated, in terms of the intracellular redox state, by oxidation of Cys-106.

TL;DR: The results showed that endogenous H(2)S system was involved in both the maintenance of basal blood pressure and the development of hypertension, and could exert beneficial effect on the pathogenesis of spontaneous hypertension.

TL;DR: It is shown for the first time that ghrelin clearly and dose dependently increases memory retention in the hippocampus, amygdala, and DRN and the highest dose of 3 nmol/microl was effective in all of them.

TL;DR: Data indicate that gAd inhibits oxLDL-induced cell proliferation and suppresses cellular superoxide generation, possibly through an NAD(P)H oxidase-linked mechanism.

TL;DR: For the first time, human adipose cells with normal karyotype and indefinite life span have been established and represent a novel and valuable tool for studies of fat tissue development and metabolism.

TL;DR: It is reported here that incubation with IL-6 increases the phosphorylation of AMPK and that of its target molecule, acetyl CoA carboxylase, in both extensor digitorum longus muscle and cultured F422a adipocytes, and that this contributes to, but does not fully account for, the increase in AMPK activity in these tissues in response to exercise.

TL;DR: In this article, a new function of apelin as a novel angiogenic factor in retinal endothelial cells was reported, where apelin at concentrations of 1 pM-1μM significantly enhanced migration, proliferation, and capillary-like tube formation of RF/6A cells, but not those of HUVECs.

TL;DR: EFV dose could be decreased in those patients harboring the genotype of CYP2B6 to reduce toxicity with compromising potency, representing the first step of the Tailor-Made therapy of HIV-1 infection.